Growth Inhibition of Human Promonocytic Leukaemic U937 Cells by Interferon Gamma Is Irreversible and Not Cell Cycle Phase-Specific

Joshi, Chirag; Supriya, Patil; Ajitkumar, Parthasarathi

doi:10.1006/cyto.1998.0474

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Proliferation of control and 231-CM-treated U937 cells was quantified using a colorimetric MTT assay as previously described [27] and cell growth curve [28]. For MTT assay, 5.0 x 10 3 cells in 100 μl of RPMI-1640 media were seeded per well in 96 well plates.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Interleukin-6 Increases Expression and Secretion of Cathepsin B by Breast Tumor-Associated Monocytes

Mohamed¹,

Rudy²,

Anbalagan³

et al. 2010

Cell Physiol Biochem

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In the tumor microenvironment, monocytes respond to paracrine stimuli from breast cancer cells by secreting molecules that participate in breast cancer growth, invasion, intravasation and metastasis. Here we examined the effects of media conditioned by MDA-MB-231 human breast carcinoma cells (231-CM) on expression and secretion of proteases and secretion of cytokines by U937 human monocytes. We found that 231-CM increased U937: 1) proliferation; 2) expression, activity and secretion of the cysteine protease cathepsin B (CTSB); 3) secretion of matrix metalloproteinases (MMP)-2 and -9; and 4) secretion of interleukin-6 (IL-6) and insulinlike growth factor binding protein-1 (IGFBP-1). We further demonstrated by western blotting and enzymatic activity assays that the increases in CTSB secretion and activity induced by 231-CM could be reduced by neutralizing antibodies against IL-6. Our data suggest a role for IL-6 in

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Section: Cell Proliferation Assaymentioning

confidence: 99%

Interleukin-6 Increases Expression and Secretion of Cathepsin B by Breast Tumor-Associated Monocytes

Mohamed¹,

Rudy²,

Anbalagan³

et al. 2010

Cell Physiol Biochem

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“…Moreover, cell cycle analyses indicated that IFN-α and IFN-β arrested the growth of EW-7 cells in the Sphase. The effects of IFNs on the cell cycle appear diverse [35][36][37][38][39][40]. For example, IFN-α inhibits the growth of Daudi cells at the G o \G " boundary of the cell cycle [35].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, IFN-α and IFN-β inhibit glioma cell growth due to accumulation of cells in the S-phase [38]. Alternatively, IFNs arrest various cell types in a cell cycle phaseindependent manner [39,40].…”

Section: Discussionmentioning

confidence: 99%

γ-Glutamyl transpeptidase expression in Ewing's sarcoma cells:up-regulation by interferons

Bouman

Sancëau

Rouillard

et al. 2002

Biochemical Journal

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The genetic hallmark of Ewing's sarcoma family of tumours (ET) is the presence of the translocation t(11;22)(q24;q12), which creates the ET fusion gene, leading to cellular transformation. Five human gamma-glutamyl transpeptidase (gamma-GT) genes are located near the chromosomal translocation in ET. gamma-GT is a major enzyme involved in glutathione homoeostasis. Five human cell lines representative of primary or metastatic tumours were investigated to study whether gamma-GT alterations could occur at the chromosomal breaks and rearrangements in ET. As shown by enzymic assays and FACS analyses, all ET cell lines consistently expressed a functional gamma-GT which however did not discriminate steps of ET progression. As shown previously [Sancéau, Hiscott, Delattre and Wietzerbin (2000) Oncogene 19, 3372-3383], ET cells respond to the antiproliferative effects of interferons (IFNs) type I (alpha and beta) and to a much less degree to IFN type II (gamma). IFN-alpha and -beta arrested cells in the S-phase of the cell cycle. We found an enhancement of gamma-GT mRNA species with IFN-alpha and -beta by reverse transcriptase-PCR analyses. This is reflected by up-regulation of gamma-GT protein, which coincides with the increase in gamma-GT-specific enzymic activity. Similarly, IFNs up-regulate the levels of gamma-GT in another IFN-responsive B cell line. Whether this up-regulation of gamma-GT by IFNs is of physiological relevance to cell behaviour remains to be studied.

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Diversity of cell phenotypes among MT-2 cell lines affects the growth of U937 cells and cytokine production

et al. 2018

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Growth Inhibition of Human Promonocytic Leukaemic U937 Cells by Interferon Gamma Is Irreversible and Not Cell Cycle Phase-Specific

Cited by 3 publications

References 13 publications

Interleukin-6 Increases Expression and Secretion of Cathepsin B by Breast Tumor-Associated Monocytes

Interleukin-6 Increases Expression and Secretion of Cathepsin B by Breast Tumor-Associated Monocytes

γ-Glutamyl transpeptidase expression in Ewing's sarcoma cells:up-regulation by interferons

Diversity of cell phenotypes among MT-2 cell lines affects the growth of U937 cells and cytokine production

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