Growth inhibition of pathogenic yeasts by <i>Pseudomonas aeruginosa in vitro</i>: clinical implications in blood cultures

Grillot, R.; Portmann-Coffin, V.; Ambroise-Thomas, P

doi:10.1111/myc.1994.37.9-10.343

Cited by 21 publications

(17 citation statements)

References 19 publications

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“…was 29.2 h, whereas the majority of bacterial strains tested presented a shorter detection time (14). It was also shown that bacterial flora inhibited detection of yeasts in 50% of polymicrobial samples inoculated on media not spiked with antibiotics (13) and that Pseudomonas aeruginosa was capable of inhibiting the growth in vitro of several yeast species (4). An in vivo model of coinfection with C. albicans and P. aeruginosa has confirmed these data (6).…”

Section: Discussionsupporting

confidence: 68%

Comparison of Mycosis IC/F and Plus Aerobic/F Media for Diagnosis of Fungemia by the Bactec 9240 System

et al. 2004

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Fungemia is associated with a high mortality rate. We compared the performance of the Mycosis IC/F selective fungal medium and the Plus Aerobic/F standard bacteriological medium for the diagnosis of fungemia on the Bactec 9240 automatic system. We retrospectively analyzed 550 blood culture pairs composed of one Mycosis IC/F vial and one Plus Aerobic/F vial, drawn in 187 patients with fungemia. The positivity rate by vial was significantly higher on Mycosis IC/F medium than on Plus Aerobic/F medium (88.0% versus 74.9%, P < 0.0001). The positivity rate for fungus detection on Plus Aerobic/F medium fell to 26.9% when bacteria were present in the same vial. The positivity rate by patient was also significantly higher on Mycosis IC/F medium than on Plus Aerobic/F medium (92.5% versus 75.9%, P < 0.0001). A marked superiority of Mycosis IC/F medium was demonstrated for diagnosis of Candida glabrata fungemia (31 of 31, 100%, versus 18 of 31, 58.1%, P < 0.0001). The mean detection time was significantly shorter on Mycosis IC/F medium than on Plus Aerobic/F medium (28.9 ؎ 22.2 h versus 36.5 ؎ 24.6 h, P < 0.0001). The mean time saving was 8.8 h for Candida albicans and 43.7 h for C. glabrata. Mycosis IC/F medium enabled more sensitive and earlier diagnosis, particularly for the two strains most frequently responsible for fungemia, C. albicans and C. glabrata, and also in the event of the concomitant presence of both yeasts and bacteria. In patients with risk factors, it would thus appear to be sensible to draw a Mycosis IC/F vial in addition to the standard bacteriological vials.

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Section: Discussionsupporting

confidence: 68%

Comparison of Mycosis IC/F and Plus Aerobic/F Media for Diagnosis of Fungemia by the Bactec 9240 System

et al. 2004

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“…In this study, we used the term ‘mixed Candida /bacterial BSI’ to describe the isolation of bacterial species within 48 h of the time of candidaemia to avoid confusion with previous studies. We chose this definition because concomitant bacteraemia may obscure the detection of fungaemia using standard blood culture techniques by suppression of fungal growth [9,10,26]. The inclusion of only synchronous candidaemia and bacteraemia could cause the underestimation of the incidence of mixed Candida /bacterial BSIs.…”

Section: Discussionmentioning

confidence: 99%

Risk factors for and clinical implications of mixed Candida/bacterial bloodstream infections

Kim¹,

Yoon²,

Kim³

et al. 2013

Clinical Microbiology and Infection

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Mixed Candida/bacterial bloodstream infections (BSIs) have been reported to occur in more than 23% of all episodes of candidaemia. However, the clinical implications of mixed Candida/bacterial BSIs are not well known. We performed a retrospective case-control study of all consecutive patients with candidaemia over a 5-year period to determine the risk factors for and clinical outcomes of mixed Candida/bacterial BSIs (cases) compared with monomicrobial candidaemia (controls). Thirty-seven (29%) out of 126 patients with candidaemia met the criteria for cases. Coagulase-negative staphylococci were the predominant bacteria (23%) in cases. In multivariate analysis, duration of previous hospital stay ≥7 weeks (odds ratio (OR), 2.86; 95% confidence interval (CI), 1.09–7.53), prior antibiotic therapy ≥7 days (OR, 0.33; 95% CI, 0.14–0.82) and septic shock at the time of candidaemia (OR, 2.60; 95% CI, 1.14–5.93) were significantly associated with cases. Documented clearance of candidaemia within 3 days after initiation of antifungal therapy was less frequent in cases (63% vs. 84%; p = 0.035). The difference in the rate of treatment failure at 2 weeks was not significant between cases (68%) and controls (62%; p = 0.55). The crude mortality at 6 weeks and survival through 100 days did not differ between the two patient groups (p = 0.56 and p = 0.80, respectively). Mixed Candida/bacterial BSIs showed a lower clearance rate of candidaemia during the early period of antifungal therapy, although the treatment response and survival rate were similar regardless of concurrent bacteraemia. Further studies on the clinical relevance of species-specific Candida-bacterial interactions are needed.

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“…According to Meyer et al (16), this selective effect of antibiotics in the Mycosis IC/F medium might well be the main reason for its advantage in yeast isolation. However, we have observed that Mycosis IC/F medium detects bacterial strains belonging to different species, including P. aeruginosa, a microorganism known for its ability to inhibit the growth of several yeast species both in vitro (8) and in vivo (9). Therefore, the possibility that bacterial inhibition of yeast isolation can also occur in the Mycosis IC/F bottles when yeasts are present concomitantly with antibiotic-resistant bacteria should be considered.…”

Section: Discussionmentioning

confidence: 99%

Detection of Bacterial and Yeast Species with the Bactec 9120 Automated System with Routine Use of Aerobic, Anaerobic, and Fungal Media

et al. 2008

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Despite recent developments in microbiological biomolecular techniques, blood culture remains the most practical and reliable method in the diagnosis of bloodstream infections and one of the most important tools in the clinical microbiology laboratory, taking into account the role played by systemic infections as a major cause of mortality and morbidity, especially within the hospital environment. Various continuously monitoring blood culture systems are extensively used in order to increase the rapidity of isolation and identification of microorganisms in blood samples, and paired aerobic and anaerobic blood culture bottles are commonly used in order to obtain better overall recovery of microorganisms, as well as the recovery of specific types of bacteria. The traditional aerobic and anaerobic bacteriological media also detect episodes of candidemia, although the sensitivity in this case is estimated to be only 50% (12). The efficiency of yeast detection in blood samples can be increased by terminal subcultures, but the practice is time-consuming and the risk of underestimating yeast infections remains high. This is unacceptable if we take into account the expanding role of yeasts as agents of nosocomial infections, particularly in intensive-care units (ICUs) (4, 18, 23), and candidemia-associated mortality (40%), the highest of all hospital bloodstream infections (4). In order to facilitate faster and more efficient isolation of fungi from blood, the Bactec automated blood culture system (Becton Dickinson Diagnostic Systems, Sparks, MD) has media specifically formulated for this purpose. In addition to the traditional aerobicanaerobic bacteriological set, their use has been recommended when blood culture is required for patients at risk of fungal infections (7, 16) and when a concomitant bloodstream bacterial infection is suspected of inhibiting yeast detection (16).Nevertheless, fungal media are not routinely employed by many hospitals, as they require additional blood from patients, an increased amount of incubator space, and the additional costs of processing and purchasing bottles (10). Moreover, even the routine use of anaerobic bacteriological bottles in blood culture has been (21), and still is (13), a subject of discussion because of the reportedly declining rates of anaerobic bloodstream infections (2) and the possibility of recognizing them clinically and treating affected patients empirically for such infections (17,19,22).

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Growth inhibition of pathogenic yeasts by Pseudomonas aeruginosa in vitro: clinical implications in blood cultures

Cited by 21 publications

References 19 publications

Comparison of Mycosis IC/F and Plus Aerobic/F Media for Diagnosis of Fungemia by the Bactec 9240 System

Comparison of Mycosis IC/F and Plus Aerobic/F Media for Diagnosis of Fungemia by the Bactec 9240 System

Risk factors for and clinical implications of mixed Candida/bacterial bloodstream infections

Detection of Bacterial and Yeast Species with the Bactec 9120 Automated System with Routine Use of Aerobic, Anaerobic, and Fungal Media

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