Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk

Abstract

Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial muscle. Two subsequent immunization steps with 21 days intervals were repeated in 0,85% saline in FIA. Blood and eggs samples were collected before and after immunization steps. Egg yolk immunoglobulins were purified by precipitation with 19% sodium sulfate and 20% ammonium sulphate methodologies. Purified IgY 50μL aliquots were incubated in 850μL BHI broth containing 50μL inoculums of five strains of S. aureus and five strains of E.coli during four hours at 37°C. Growth inhibition was evaluated followed by photometry reading (DO550nm). Egg yolk IgY preparation from hiperimmunized birds contained antibodies that inhibited significantly (p<0,05) growth of strains tested. Potential use of ostrich IgY polyclonal antibodies as a diagnostic and therapeutic tool is proposed for diseased animals.

Egg Yolk IgY: A Novel Trend of Feed Additives to Limit Drugs and to Improve Poultry Meat Quality

“…The determination of antimicrobial activities of either experimented IgY or probiotic (500 µl each) and a mixture of both (250 µl each) in vitro revealed that IgY can also retain a bactericidal activity against gram-positive bacteria including S. aureus and L. monocytogenes at a concentration of 1,500 µg/ml. These findings coincided with a previous report that showed an effective bactericidal activity of IgY against S. aureus growth, suggesting the therapeutic benefits of IgY (33). This result was also consistent with a previous study (34) that showed the ability of IgY to suppress Staphylococcus in chicken meat.…”

IgY technology: Methods for developing and evaluating avian immunoglobulins for the in vitro detection of biomolecules

“…Ostrich and quail immunoglobulins Y were characterized in terms of their molecular weight (SDS-PAGE and western-blotting) and their ability to recognize and bind to a commercially available horseradish peroxidase (HRP)-labeled rabbit anti-hen IgY antibody in an ELISA system[ 120 ]. As revealed, the ostrich IgYs could be hardly recognized by the HRP-labeled anti-hen antibody we used, though other researchers reported successful use of commercially available secondary anti-hen antibodies to assess the immunochemical efficiency of specific ostrich IgYs[ 121 ]. On the other hand, HRP-labeled secondary anti-ostrich-IgY antibodies have been specially developed and used to evaluate ostrich IgYs with ELISA[ 25 ].…”

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

“…The inhibiting growth activity of IgY anti-Streptococcus mitis was preserved by extracting yolk antibodies by precipitation with PEG 6000 and AmS and further isolation by DEAE cellulose ion exchange column chromatography [34]. Similarly, growth inhibition of Staphylococcus aureus and Escherichia coli was obtained with yolk antibodies isolated by NaS and AmS precipitations [35].…”