Growth inhibition of the harmful alga Prymnesium parvum by plant-derived products and identification of ellipticine as highly potent allelochemical

Mary, Mousumi A.; Rashel, Rakib H.; Patiño, Reynaldo

doi:10.1007/s10811-021-02545-6

Cited by 6 publications

(16 citation statements)

References 51 publications

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“…Prymnesium parvum (UTEX-2797) was obtained from UTEX Culture Collection of Algae (The University of Texas at Austin, TX, USA). Stock cultures were maintained as previously described [15]. Briefly, nonaxenic cultures were maintained in modified UTEX artificial seawater medium at 5 psu and f/2 levels of nutrients (modified ASM) and placed in a plant germination rack with two full-spectrum LED lights.…”

Section: General Culture Methodsmentioning

confidence: 99%

“…The latter procedure was applied to minimize the presence of debris and bacterial contamination in the cultures. Ellipticine (324,688, Sigma-Aldrich) was prepared following procedures previously described [15]. Dimethyl sulfoxide (DMSO) (D4540, Sigma-Aldrich) was used as carrier solution at a final concentration of 0.05% in experimental media.…”

Section: Experimental Reagentsmentioning

confidence: 99%

“…Ambient culture conditions were the same as described for stock cultures. Cells were counted at 24 h of incubation by hemocytometry [15]. Each exposure treatment was conducted in triplicate flasks.…”

Section: Giant Reed Exposuresmentioning

confidence: 99%

“…More recently, a methanolic extract of giant reed (Arundo donax) was shown to suppress P. parvum growth in a concentration-dependent manner [13]. A follow-up study reported that an indole alkaloid found in giant reed, ellipticine [14], has highly potent algicidal activity against P. parvum [15]. Various extract preparations from giant reed can also inhibit growth of other HAB species, such as the cyanobacterium Microcystis aeruginosa [14,[16][17][18] and the marine dinoflagellate Karenia brevis [19].…”

Section: Introductionmentioning

confidence: 99%

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Plant-Derived Products Selectively Suppress Growth of the Harmful Alga Prymnesium parvum

Mary,

Tábora-Sarmiento,

Nash

et al. 2024

Water

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Prymnesium parvum is a harmful alga found in brackish waters worldwide whose toxins can be lethal to aquatic organisms. Established field methods to control blooms of this species, however, are unavailable. Earlier studies showed that various extracts of giant reed (Arundo donax) can suppress P. parvum growth and that ellipticine, an allelochemical present in giant reed, is a potent algicide against this species. The unintended effects of giant reed products on nontarget organisms, however, are not fully understood. This study determined the effects of giant reed leachate (aqueous extract of dried chips) and ellipticine on growth of P. parvum and the green microalga Chlorella sorokiniana; survival and reproduction of the planktonic crustacean Daphnia pulex; and hatching success, larval survival, and larval swimming behavior of the teleost fish Danio rerio. Leachate made with 3 g chips L−1 was lethally toxic to P. parvum and D. pulex, stimulated C. sorokiniana growth, and impaired D. rerio behavior. Leachate at 1 g L−1 fully suppressed P. parvum growth, had moderate effects on D. pulex reproductive output, and had no effects on D. rerio. Ellipticine at 0.01 mg L−1 irreversibly inhibited P. parvum growth, acutely but reversibly inhibited C. sorokiniana growth, slightly delayed D. pulex reproduction, and had no effects on D. rerio. These observations suggest that when applied at appropriate concentrations, natural products derived from giant reed can be used as tools to specifically control P. parvum growth with minimal effects on nontarget species.

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Section: General Culture Methodsmentioning

confidence: 99%

Section: Experimental Reagentsmentioning

confidence: 99%

Section: Giant Reed Exposuresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plant-Derived Products Selectively Suppress Growth of the Harmful Alga Prymnesium parvum

Mary,

Tábora-Sarmiento,

Nash

et al. 2024

Water

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“…Furthermore, alkaloids impair the fitness of herbivorous arthropods, such as grasshoppers and aphids, inhibiting their growth and acting as a feeding deterrent [ 40 , 41 ]. Additional organisms affected by gramine include bacteria, algae and fungi [ 42 , 43 , 44 , 45 ]. Gramine and hordenine may be interesting for crop protection; however, high concentrations of these compounds pose a health risk to humans and the animals that forage on them.…”

Section: Introductionmentioning

confidence: 99%

A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

Leite Dias,

Garibay-Hernández,

Brendel

et al. 2023

Plants

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Barley (Hordeum vulgare) is one of the most widely cultivated crops for feedstock and beer production, whereas lupins (Lupinus spp.) are grown as fodder and their seeds are a source of protein. Both species produce the allelopathic alkaloids gramine and hordenine. These plant-specialized metabolites may be of economic interest for crop protection, depending on their tissue distribution. However, in high concentrations they pose a health risk to humans and animals that feed on them. This study was carried out to develop and validate a new method for monitoring these alkaloids and their related metabolites using fluorescence detection. Separation was performed on an HSS T3 column using slightly acidified water-acetonitrile eluents. Calibration plots expressed linearity over the range 0.09–100 pmol/µL for gramine. The accuracy and precision ranged from 97.8 to 123.4%, <7% RSD. The method was successfully applied in a study of the natural range of abundance of gramine, hordenine and their related metabolites, AMI, tryptophan and tyramine, in 22 barley accessions and 10 lupin species. This method provides accurate and highly sensitive chromatographic separation and detection of tryptophan- and tyrosine-derived allelochemicals and is an accessible alternative to LC-MS techniques for routine screening.

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Broad spectrum and species specificity of plant allelochemicals 1,2-benzenediol and 3-indoleacrylic acid against marine and freshwater harmful algae

Hu,

Effiong,

Liu

et al. 2023

Science of The Total Environment

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Growth inhibition of the harmful alga Prymnesium parvum by plant-derived products and identification of ellipticine as highly potent allelochemical

Cited by 6 publications

References 51 publications

Plant-Derived Products Selectively Suppress Growth of the Harmful Alga Prymnesium parvum

Plant-Derived Products Selectively Suppress Growth of the Harmful Alga Prymnesium parvum

A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin

Broad spectrum and species specificity of plant allelochemicals 1,2-benzenediol and 3-indoleacrylic acid against marine and freshwater harmful algae

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