Growth Inhibitory and Antimetastatic Effect of Green Tea Polyphenols on Metastasis-Specific Mouse Mammary Carcinoma 4T1 Cells <i>In vitro</i> and <i>In vivo</i> Systems

Baliga, Manjeshwar Shrinath; Meleth, Sreelatha; Katiyar, Säntosh K.

doi:10.1158/1078-0432.ccr-04-1976

Cited by 154 publications

(115 citation statements)

References 47 publications

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“…In this study, we identified that EGCG, the major polyphenolic agent present in green tea, inhibited the growth of lung cancer A549 cells in vivo, which was consistent with previous studies indicating that EGCG inhibited growth and apoptosis in human lung, colon, gastric, prostate and mammary carcinoma (19)(20)(21)(22)(23). At present, a number of agents are used to clinically treat carcinoma, including cisplatin, gemcitabine and paclitaxel.…”

Section: Discussionsupporting

confidence: 90%

Role of Ku70 and Bax in epigallocatechin-3-gallate-induced apoptosis of A549 cells in vivo

et al. 2012

Oncology Letters

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Abstract. EGCG (epigallocatechin-3-gallate), the major catechin found in green tea, has been demonstrated to inhibit proliferation and induce apoptosis in a number of types of tumors. Recent studies reveal that EGCG has various anticancer effects. This study investigated a further possible molecular mechanism of the anticancer effects of EGCG in murine lung cancer xenografts. In the study, A549 human lung cancer cells were injected into nude mice. Tumor volume was used to measure cancer cell growth. The weight of the animals was used to assess the toxicity of the drugs. The expression of protein and mRNA was assayed by western blot analysis and RT-PCR, respectively. The interaction between Bax and Ku70 was determined by immunoprecipitation. Our results suggest that EGCG induced A549 lung cancer cell apoptosis in vivo, and had less toxic effects compared to classical anticancer drugs. EGCG may inhibit the surrogate markers of proliferation and apoptosis (caspase 3) in A549 tumor xenografts in vivo. In addition, EGCG downregulated the expression of Bcl-xl and upregulated the expression of Bax mRNA and protein. Further experiments indicated that EGCG downregulated the protein expression of Ku70 and interrupted the binding of Ku70 and Bax. This is the first study demonstrating that the induction of apoptosis by EGCG may be caused by the downregulation of Ku70 and that EGCG disrupts the interaction between Ku70 and Bax in lung cancer.

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Section: Discussionsupporting

confidence: 90%

Role of Ku70 and Bax in epigallocatechin-3-gallate-induced apoptosis of A549 cells in vivo

et al. 2012

Oncology Letters

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“…14,15 Tea polyphenols, particularly green tea polyphenols, have been demonstrated to possess anticarcinogenic effects against breast cancer in experimental. 16 Epidemiologic data reported that green tea was inversely associated with breast cancer risk including one of our studies. 17,18 In view of the variations in rates of breast cancer and patterns of dietary practices, as well as the separately emerging bodies of evidence on the possible cancer-protective properties of mushrooms and green tea, it is important to investigate whether the joint effect of mushrooms and green tea is synergistic on breast cancer, which may explain the lower incidence of breast cancer in China.…”

mentioning

confidence: 54%

Dietary intakes of mushrooms and green tea combine to reduce the risk of breast cancer in Chinese women

Zhang

Huang

Xie

et al. 2008

Intl Journal of Cancer

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To investigate effects of dietary mushrooms and joint effects of mushrooms and green tea on breast cancer, a case-control study was conducted in southeast China in [2004][2005]. The incident cases were 1,009 female patients aged 20-87 years with histologically confirmed breast cancer. The 1,009 age-matched controls were healthy women randomly recruited from outpatient breast clinics. Information on frequency and quantity of dietary intake of mushrooms and tea consumption, usual diet, and lifestyle were collected by face-to-face interview using a validated and reliable questionnaire. Compared with nonconsumers, the Odds ratios (Ors) were 0.36 (95% CI 5 0.25-0.51) and 0.53 (0.38-0.73) for daily intake of 10 g fresh mushrooms and 4 g dried mushrooms, based on multivariate logistic regression analysis adjusting for established and potential confounders. There were doseresponse relationships with significant tests for trend (p < 0.001). The inverse association was found in both pre-and postmenopausal women. Compared with those who consumed neither mushrooms nor green tea, the ORs were 0.11 (0.06-0.20) and 0.18 (0.11-0.29) for daily high intake of fresh and dried mushrooms combined with consuming beverages made from 1.05 g dried green tea leaves per day. The corresponding linear trends were statistically significant for joint effect (p < 0.001). We conclude that higher dietary intake of mushrooms decreased breast cancer risk in pre-and postmenopausal Chinese women and an additional decreased risk of breast cancer from joint effect of mushrooms and green tea was observed. More research is warranted to examine the effects of dietary mushrooms and mechanism of joint effects of phytochemicals on breast cancer.

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“…In our laboratory, we have found that lycopene delivered at physiological concentrations can induce cytochrome c release in human prostate cells, and, in fact, concentrations equivalent to the plasma level found in those consuming three to five daily servings of fruits and vegetables also induced this change (34). Green tea polyphenols (i.e., EGCG) induced cytochrome c release in vitro and in vivo in metastatic mouse mammary carcinoma cells, as well as altered Bcl-2/Bax protein ratios, increased Apaf formation, and cleaved caspase and poly(ADP-ribose) polymerase (PARP) proteins (71). Dietary ginger, including curcumin, 6-gingerol, and other diterpenes, induced apoptosis in T lymphocytes by inducing alteration of MMP and increasing cytochrome c release (72).…”

Section: Regulation Of Apoptosismentioning

confidence: 97%

Targeting Apoptosis with Dietary Bioactive Agents

Martin

2006

Exp Biol Med (Maywood)

123

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Apoptosls, a form of programmed cell death, Is a pivotal defense against the occurrence of cancer and is essential to metazoans In maintaining tissue homeostasis. Apoptosls exhibits a distinctive phenotype and Involves elimination of potentially deleterious cells. Many diseases have been associated with aberrantly regulated apoptotlc cell death, ultimately leading to Inhibition of apoptosls and propagation of diseases such as cancer. Elucidation of the critical events associated with carcinogenesis provides the opportunity for dietary Intervention to prevent cancer development through Induction of apoptosls, particularly by bloactive agents or functional foods. Diet Is a significant environmental factor In the overall cancer process and can exacerbate or Interfere with carcinogenesis. Apoptosls occurs primarily through two well-recognized pathways In cells, Inciudlng the Intrinsic, or mitochondrial-mediated, effector mechanism and the extrinsic, or death receptor-mediated, effector mechanism. In addition to diet's effects on protein expression and function, evidence Is also accumulating that a large number of dietary food components can exert effects on the human genome, either directly or Indirectly, to modUlate gene expression. In fact, many diet-related genes are Involved In carcinogenesis as well as apoptosls, and thus are ultimately molecular targets for dietary chemopreventlon. There are multiple steps within pathways In which dietary components can alter gene expression and phenotypes of cells and thus Influence cancer outcomes (nutritional transcrlptomlc effect). Thus, apoptosls Is an emerging therapeutic target of bloactlve agents of diet. In this review, the process of apoptosls Is discussed and the potential mechanistic Interaction of bloactlve agents, as

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Growth Inhibitory and Antimetastatic Effect of Green Tea Polyphenols on Metastasis-Specific Mouse Mammary Carcinoma 4T1 Cells In vitro and In vivo Systems

Cited by 154 publications

References 47 publications

Role of Ku70 and Bax in epigallocatechin-3-gallate-induced apoptosis of A549 cells in vivo

Role of Ku70 and Bax in epigallocatechin-3-gallate-induced apoptosis of A549 cells in vivo

Dietary intakes of mushrooms and green tea combine to reduce the risk of breast cancer in Chinese women

Targeting Apoptosis with Dietary Bioactive Agents

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