Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line

Hudson, E A; Howells, Lynne; Gallacher-Horley, Barbara; Fox, Louise H.; Gescher, Andreas J.; Manson, Margaret M.

doi:10.1186/1471-2407-3-2

Cited by 34 publications

(16 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These indole derivatives have been shown to suppress the proliferation of various cancer cell lines at the concentration range between 1 and 100 mM. These include those of breast [14][15][16][17], colon [18][19][20], and prostate [21][22][23], by targeting a wide spectrum of signaling pathways governing hormonal homeostasis, cell-cycle progression, and cell proliferation and survival [24][25][26][27][28]. Previous studies have also shown DIM and I3C can suppress the proliferation of prostate cancer cells [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Broccoli‐derived phytochemicals indole‐3‐carbinol and 3,3′‐diindolylmethane exerts concentration‐dependent pleiotropic effects on prostate cancer cells: Comparison with other cancer preventive phytochemicals

Wang

Schoene

Milner

et al. 2011

Molecular Carcinogenesis

View full text Add to dashboard Cite

In the present studies, we utilized prostate cancer cell culture models to elucidate the mechanisms of action of broccoli-derived phytochemicals 3,3'-diindolylmethane (DIM) and indole-3-carbinol (I3C). We found DIM and I3C at 1-5 µM inhibited androgen and estrogen-mediated pathways and induced xenobiotic metabolism pathway. By contrast, DIM and I3C induced cyclin inhibitors, indicators of stress/DNA damage, only at ≥25 µM. We also demonstrated that an inhibitory effect of DIM and I3C on cell growth involves inhibition of insulin-like growth factor-1 receptor expression. More importantly, we showed that differences in efficacies and mechanisms existed between DIM and I3C. These included differences in effective concentrations, a differential effect on androgen receptor binding, and a differential effect on xenobiotic metabolic pathway through aryl hydrocarbon receptor-dependent and -independent mechanism. Furthermore we determined that several other diet-derived cancer protective compounds, similar to DIM and I3C, exhibited pleiotrophic effects on signaling pathways that included proliferation, cell cycle, and nuclear receptors-mediated pathways. However, the efficacies and mechanisms of these compounds vary. We also showed that some cellular pathways are not likely to be affected by DIM or I3C when circulating concentration of orally ingested DIM or I3C is considered. Based on our results, a model for cancer protective effects of DIM and I3C was proposed.

show abstract

Section: Introductionmentioning

confidence: 99%

Broccoli‐derived phytochemicals indole‐3‐carbinol and 3,3′‐diindolylmethane exerts concentration‐dependent pleiotropic effects on prostate cancer cells: Comparison with other cancer preventive phytochemicals

Wang

Schoene

Milner

et al. 2011

Molecular Carcinogenesis

View full text Add to dashboard Cite

show abstract

“…I3C has been shown to suppress the proliferation of a wide variety of cells, including breast cancer cells, [9][10][11][12] colon cancer cells, [13][14][15] prostate cancer cells, 16,17 and endometrial cancer cells. 18 Katdare's group showed that I3C inhibits benzopyrene-induced proliferation of mammary epithelial cells.…”

Section: Antiproliferative Effects Of I3c Against Tumor Cellsmentioning

confidence: 99%

Molecular Targets and Anticancer Potential of Indole-3-Carbinol and Its Derivatives

2005

View full text Add to dashboard Cite

Previously published online as a Cell Cycle E-publication: http://www.landesbioscience.com/journals/cc/abstract.php?id=1993 KEY WORDSI3C ReviewMolecular Targets and Anticancer Potential of Indole-3-Carbinol and Its Derivatives ABSTRACT Indole-3-carbinol (I3C) is produced by members of the family Cruciferae, and particularly members of the genus Brassica (e.g., cabbage, radishes, cauliflower, broccoli, Brussels sprouts, and daikon). Under acidic conditions, I3C is converted to a series of oligomeric products (among which 3,3'-diindolylmethane is a major component) thought to be responsible for its biological effects in vivo. In vitro, I3C has been shown to suppress the proliferation of various tumor cells including breast cancer, prostate cancer, endometrial cancer, colon cancer and leukemic cells; induce G 1 /S arrest of the cell cycle and induce apoptosis. The cell cycle arrest involves downregulation of cyclin D1, cyclin E, cyclin-dependent kinase (CDK)2, CDK4 and CDK6 and upregulation of p15, p21 and p27. Apoptosis by I3C involves downregulation antiapoptotic gene products, including Bcl-2, Bcl-xL, survivin, inhibitor-of-apoptosis protein (IAP), X chromosome-linked IAP (XIAP), and Fas-associated death domain protein-like interleukin-1-β-converting enzyme inhibitory protein (FLIP); upregulation of proapoptotic protein Bax; release of micochondrial cytochrome C; and activation of caspase-9 and caspase-3. This agent inhibits the activation of various transcription factors including nuclear factor-kappaB, SP1, estrogen receptor, androgen receptor and nuclear factor-E2-related factor 2 (Nrf2). This indole potentiates the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through induction of death receptors and synergises with chemotherapeutic agents through downregulation of P-glycoprotein (P-gp). In vivo, I3C was found to be a potent chemopreventive agent for hormonal-dependent cancers such as breast and cervical cancer. These effects are mediated through its ability to induce apoptosis, inhibit DNA-carcinogen adduct formation, and suppress free-radical production, stimulate 2-hydroxylation of estradiol, inhibit invasion and angiogenesis. Numerous studies have indicated that I3C also has a strong hepatoprotective activity against various carcinogens. Initial clinical trials in women have shown that I3C is a promising agent against breast and cervical cancers.

show abstract

“…In addition to Mig-7 mRNA being produced under the CMV promoter in this cell line, ODC activity is a log higher than those found in other carcinoma cell lines. As a result, HT29 cells are frequently used to study ODC activities (Chi et al, 2006;Hudson et al, 2003;Martinez et al, 2003;Selamnia et al, 1998). Parental HT29 cells do not express Mig-7 mRNA due to a lack of signaling from αvβ5 integrin (Crouch et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Translation of an atypical human cDNA requires fidelity of apurine-pyrimidine repeat region and recoding

Petty

Dick

Lindsey

2008

Gene

View full text Add to dashboard Cite

Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line

Cited by 34 publications

References 54 publications

Broccoli‐derived phytochemicals indole‐3‐carbinol and 3,3′‐diindolylmethane exerts concentration‐dependent pleiotropic effects on prostate cancer cells: Comparison with other cancer preventive phytochemicals

Broccoli‐derived phytochemicals indole‐3‐carbinol and 3,3′‐diindolylmethane exerts concentration‐dependent pleiotropic effects on prostate cancer cells: Comparison with other cancer preventive phytochemicals

Molecular Targets and Anticancer Potential of Indole-3-Carbinol and Its Derivatives

Translation of an atypical human cDNA requires fidelity of apurine-pyrimidine repeat region and recoding

Contact Info

Product

Resources

About