Growth kinetic study of Tetratrichomonas didelphidis isolated from opossum Lutreolina crassicaudata and interaction with a prokaryotic cell

Abstract

Tetratrichomonas didelphidis is a flagellate protozoan found in the intestine, cecum and colon of opossums, Didelphis marsupialis. This work reports the occurrence of T. didelphidis in another opossum species, Lutreolina crassicaudata. The strain was cultivated in monoxenic culture with Escherichia coli in Diamond (TYM) medium without maltose and with starch solution (trypticase-yeast extract-starch), pH 7.5 at 28 degrees C. The growth kinetic study of T. didelphidis showed a longer time of growth and a higher number of trophozoites when inoculated with E. coli than in axenic cultures, in aerobiosis as well as under anaerobic conditions. Scanning electron microscopy showed that the bacteria adhered throughout the protozoan body and probably evoked endocytic channels, strongly suggesting the existence of endocytosis of rods by T. didelphidis. Our preliminary results suggest that the in vitro culture of T. didelphidis depends on E. coli as a growth-promoting partner, and requires monoxenic cultivation.

Systematics, biology and invasiology of trichomonads (Trichomonadida) - the current state of knowledge

“…Chorobotwórczość poszczególnych gatunków rzęsistków, a nawet ich szczepów jest różna i zależy od wielu czynników (50,53). Gatunki, których typowym miejscem lokalizacji jest jelito grube, w większości wydają się niepatogenne lub mało patogenne, podczas gdy lokalizacja pozajelitowa rzęsistków często prowadzi do rozwoju choroby u żywiciela (88,90).…”

“…Odkrywane są nowe gatunki, a istnienie innych jest podważane (31,64,68,90). Według zebranych dotychczas informacji, do najliczniejszych gatunkowo rodzajów należą Monocercomonas oraz Tetratrichomonas.…”

Tritrichomonas foetus: a scanning electron microscopy study of erythrocyte adhesion associated with hemolytic activity

“…The cells were then allowed to adhere to glass coverslips previously coated with 0.1% poly-L-lysine (Sigma, St. Louis, MO, USA) and post-fixed for 2 h with 1% OsO 4 . The fixed samples were dehydrated in ethanol, critically point dried with a Bal-Tec CPD 030 critical-point dryer and lightly coated with gold particles in a Bal-Tec SCD 005 sputter coater [11,29,34]. Observations were carried out using a Philips XL30 scanning electron microscope (SEM).…”

Electron microscopic study of Tetratrichomonas didelphidis and its interaction with a prokaryotic cell

“…This species presented a longer growth time and a higher number of trophozoites when inoculated with E. coli than in axenic cultures, in aerobiosis as well as under anaerobic conditions. We also showed, by scanning electron microscopy (SEM), that the bacteria adhered throughout the protozoan body and probably evoked endocytic channels, strongly suggesting the existence of endocytosis of rods by T. didelphidis (Tasca and De Carli 2001). Taking into account the importance of monoxenic cultivation of T. didelphidis with E. coli as a growth-promoting partner, the aim of this study was to investigate the interaction …”