Growth kinetics and stable carbon isotope fractionation during aerobic degradation of cis-1,2-dichloroethene and vinyl chloride

Tiehm, Andreas; Schmidt, Kathrin R.; Pfeifer, Brigitte; Heidinger, Michael; Ertl, Siegmund

doi:10.1016/j.watres.2008.01.029

Cited by 41 publications

(30 citation statements)

References 27 publications

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“…These values correspond to KIE values of 1.007 to 1.008, which are very close to the expected value for epoxidation (1.011) (56). For cDCE, the KIE values in this study differ significantly from previously reported values for cometabolic oxidation in studies that reported nonresolvable fractionation (6) or very little fractionation (Ϫ7.1 to Ϫ9.8‰) (59). The latter values correspond to KIE values of 1.007 to 1.010, again consistent with fractionation observed during biodegradation of VC via epoxidation and confirmed by abiotic experiments (56).…”

Section: Vol 75 2009supporting

confidence: 85%

Proteomic and Transcriptomic Analyses Reveal Genes Upregulated bycis-Dichloroethene inPolaromonassp. Strain JS666

Jennings

Chartrand

Lacrampe-Couloume

et al. 2009

Appl Environ Microbiol

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Polaromonas sp. strain JS666 is the only bacterial isolate capable of using cis-dichloroethene (cDCE) as a sole carbon and energy source. Studies of cDCE degradation in this novel organism are of interest because of potential bioremediation and biocatalysis applications. The primary cellular responses of JS666 to growth on cDCE were explored using proteomics and transcriptomics to identify the genes upregulated by cDCE. Two-dimensional gel electrophoresis revealed upregulation of genes annotated as encoding glutathione Stransferase, cyclohexanone monooxygenase, and haloacid dehalogenase. DNA microarray experiments confirmed the proteomics findings that the genes indicated above were among the most highly upregulated by cDCE. The upregulation of genes with antioxidant functions and the inhibition of cDCE degradation by elevated oxygen levels suggest that cDCE induces an oxidative stress response. Furthermore, the upregulation of a predicted ABC transporter and two sodium/solute symporters suggests that transport is important in cDCE degradation. The omics data were integrated with data from compound-specific isotope analysis (CSIA) and biochemical experiments to develop a hypothesis for cDCE degradation pathways in JS666. The CSIA results indicate that the measured isotope enrichment factors for aerobic cDCE degradation ranged from ؊17.4 to ؊22.4‰. Evidence suggests that cDCE degradation via monooxygenase-catalyzed epoxidation (CAC cleavage) may be only a minor degradation pathway under the conditions of these experiments and that the major degradation pathway involves carbon-chloride cleavage as the initial step, a novel mechanism. The results provide a significant step toward elucidation of cDCE degradation pathways and enhanced understanding of cDCE degradation in JS666.

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Section: Vol 75 2009supporting

confidence: 85%

Proteomic and Transcriptomic Analyses Reveal Genes Upregulated bycis-Dichloroethene inPolaromonassp. Strain JS666

Jennings

Chartrand

Lacrampe-Couloume

et al. 2009

Appl Environ Microbiol

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“…The oxidative VC dechlorinating culture was enriched from groundwater of a chloroethene contaminated site [25,26]. Microorganisms were transferred in modified mineral medium according to Lochhead and Chase [27] and enriched with vinyl chloride as substrate and oxygen as electron acceptor at room temperature.…”

Section: Vinyl Chloride Degrading Mixed Culturementioning

confidence: 99%

Effects of direct electric current and electrode reactions on vinyl chloride degrading microorganisms

Tiehm¹,

Lohner²,

Augenstein³

2009

Electrochimica Acta

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“…and Bacillus sp. [34,35]. Using cDCE as auxiliary substrate for growth is much less [35] and not shown in TCE and PCE.…”

Section: Aerobic Oxidative Degradationmentioning

confidence: 96%

“…Even cDCE can be cometabolized when VC is metabolic degraded [34]. Cometabolic degradation of TCE, DCE and VC is common [37,38].…”

Section: Aerobic Oxidative Degradationmentioning

confidence: 99%

Microbial Degradation of Some Halogenated Compounds: Biochemical and Molecular Features

Peng¹,

Shih²

2013

Biodegradation of Hazardous and Special Products

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Growth kinetics and stable carbon isotope fractionation during aerobic degradation of cis-1,2-dichloroethene and vinyl chloride

Cited by 41 publications

References 27 publications

Proteomic and Transcriptomic Analyses Reveal Genes Upregulated bycis-Dichloroethene inPolaromonassp. Strain JS666

Proteomic and Transcriptomic Analyses Reveal Genes Upregulated bycis-Dichloroethene inPolaromonassp. Strain JS666

Effects of direct electric current and electrode reactions on vinyl chloride degrading microorganisms

Microbial Degradation of Some Halogenated Compounds: Biochemical and Molecular Features

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