Growth Kinetics and Toxicity of <i>Pseudomonas fredriksbergsis</i> Grown on Phenol as Sole Carbon Source

Abstract

Phenol is one of the main pollutants that have a serious impact on the environment and can even be very critical to human health. The biodegradation of phenol can be considered an increasingly important pollution control process. In this study, the degradation of phenol by Pseudomonas fredriksbergsis was investigated for the first time under different growth conditions. Six different initial concentrations of phenol were used as the primary substrate. Culture conditions had an important effect on these cells' ability to biodegrade phenol. The best growth of this organism and its highest biodegradation level of phenol were noticed at pH 7, temperature 28 °C, and periods of 36 and 96 h, respectively. The highest biodegradation rate was perceived at 700 mg/L initial phenol concentration. Approximately 90% of the phenol (700 mg/L) was removed in less than 96 hours of incubation time. It was found that the Haldane model best fitted the relationship between the specific growth rate and the initial phenol concentration, whereas the phenol biodegradation profiles time could be adequately described by the modified Gompertz model. The parameters of the Haldane equation are: 0.062 h −1 , 11 ppm, and 121 ppm for Haldane's maximum specific growth rate, the half-saturation coefficient, and the Haldane's growth kinetics inhibition coefficient, respectively. The Haldane equation fitted the experimental data by minimizing the sum of squared error (SSR) to 1.36×10 -3 .

Isolation and Characterization of Yarrowia lipolytica YQ22 from Diesel Samples for Phenol Biodegradation

“…Furthermore, temperature showed a significant difference of 0.028 among the treatments (P < 0.05), indicating that maximum phenol degradation occurred at 30°C. This is due to the fact that temperature also influences enzyme activities, playing a role as important as, or even greater than, available nutrients during the degradation of organic compounds, including phenol (Aljbour et al, 2021). Furthermore, temperature showed a significant difference of 0.028 among the treatments (P < 0.0 ating that maximum phenol degradation occurred at 30°C.…”

Modeling phenol biodegradation with Pantoea agglomerans as plant-growth-promoting bacteria &lrm;

“…Because of the widespread application of phenols in the United States as well as the potential toxicity of these chemicals, the Environmental Protection Agency of the United States has placed them on the data of highest-priority pollutants [12][13][14]. There are serious drawbacks when removing phenol from the environment using techniques such as abiotic treatment, solvent extraction, chemical oxidation, burning, and adsorption [15][16][17]. These drawbacks include economic concerns as well as the development of harmful byproducts.…”

Implications of Bacterial Adaptation to Phenol Degradation under Suboptimal Culture Conditions Involving Stenotrophomonas maltophilia KB2 and Pseudomonas moorei KB4

“…The particular focus on the microbial degradation of phenol in recent years has resulted in the isolation, culture, adaptation, and enrichment of many microorganisms that can grow on this compound as a sole carbon and energy source. Among bacteria, the following new strains: Pseudomonas fredriksbergsis [13], Bacillus sp. IARI-J-20 [14], Pseudomonas putida P53 and Arthrobacter scleromae P69 [15], Rhodococcus aetherivorans [16], Rhodococcus pyridinivorans [17], and Glutamicibacter nicotianae MSSRFPD35 [11] can aerobically degrade phenol in the concentration range from 225 to 1800 mg/L.…”