Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Bainotti, A. E.; Nishio, Naomichi

doi:10.1046/j.1365-2672.2000.00854.x

Cited by 23 publications

(7 citation statements)

References 26 publications

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“…The fraction of electrons invested in biomass is quantified by f o s , which has units of e À eq to biomass per e À eq removed from the donor substrate (Rittmann and McCarty, 2001). For our analysis, we used f o s values of 0.05 for G. sulfurreducens (Bond and Lovley, 2003;Esteve-Nunez et al, 2005) as a representative for ARB, 0.08 for hydrogenotrophic methanogens (Rittmann and McCarty, 2001), 0.1 for the ethanol fermenters (adapted from Thauer et al, 1968), and we assumed an f o s 0.1 for homo-acetogens (Bainotti and Nishio, 2000;Tschech and Pfenning, 1984).…”

Section: Framework For Interpreting Electron Flow From Ethanol To Varmentioning

confidence: 99%

Syntrophic interactions among anode respiring bacteria (ARB) and Non‐ARB in a biofilm anode: electron balances

Parameswaran

Torres

Lee

et al. 2009

Biotech & Bioengineering

224

149

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We demonstrate that the coulombic efficiency (CE) of a microbial electrolytic cell (MEC) fueled with a fermentable substrate, ethanol, depended on the interactions among anode respiring bacteria (ARB) and other groups of micro-organisms, particularly fermenters and methanogens. When we allowed methanogenesis, we obtained a CE of 60%, and 26% of the electrons were lost as methane. The only methanogenic genus detected by quantitative real-time PCR was the hydrogenotrophic genus, Methanobacteriales, which presumably consumed all the hydrogen produced during ethanol fermentation ( approximately 30% of total electrons). We did not detect acetoclastic methanogenic genera, indicating that acetate-oxidizing ARB out-competed acetoclastic methanogens. Current production and methane formation increased in parallel, suggesting a syntrophic interaction between methanogens and acetate-consuming ARB. When we inhibited methanogenesis with 50 mM 2-bromoethane sulfonic acid (BES), the CE increased to 84%, and methane was not produced. With no methanogenesis, the electrons from hydrogen were converted to electrical current, either directly by the ARB or channeled to acetate through homo-acetogenesis. This illustrates the key role of competition among the various H(2) scavengers and that, when the hydrogen-consuming methanogens were present, they out-competed the other groups. These findings also demonstrate the importance of a three-way syntrophic relationship among fermenters, acetate-consuming ARB, and a H(2) consumer during the utilization of a fermentable substrate. To obtain high coulombic efficiencies with fermentable substrates in a mixed population, methanogens must be suppressed to promote new interactions at the anode that ultimately channel the electrons from hydrogen to current.

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Section: Framework For Interpreting Electron Flow From Ethanol To Varmentioning

confidence: 99%

Syntrophic interactions among anode respiring bacteria (ARB) and Non‐ARB in a biofilm anode: electron balances

Parameswaran

Torres

Lee

et al. 2009

Biotech & Bioengineering

224

149

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“… 2008 ). This different behavior is probably due to the high cobalt dependence (Bainotti and Nishio 2000 ), but slightly nickel dependence of the enzymatic system of acetogenesis (Diekert et al. 1981 ).…”

Section: Discussionmentioning

confidence: 99%

Role of nickel in high rate methanol degradation in anaerobic granular sludge bioreactors

et al. 2008

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The effect of nickel deprivation from the influent of a mesophilic (30°C) methanol fed upflow anaerobic sludge bed (UASB) reactor was investigated by coupling the reactor performance to the evolution of the Methanosarcina population of the bioreactor sludge. The reactor was operated at pH 7.0 and an organic loading rate (OLR) of 5-15 g COD l -1 day -1 for 191 days. A clear limitation of the specific methanogenic activity (SMA) on methanol due to the absence of nickel was observed after 129 days of bioreactor operation: the SMA of the sludge in medium with the complete trace metal solution except nickel amounted to 1.164 (±0.167) g CH 4 -COD g VSS -1 day -1 compared to 2.027 (±0.111) g CH 4 -COD g VSS -1 day -1 in a medium with the complete (including nickel) trace metal solution. The methanol removal efficiency during these 129 days was 99%, no volatile fatty acid (VFA) accumulation was observed and the size of the Methanosarcina population increased compared to the seed sludge. Continuation of the UASB reactor operation with the nickel limited sludge lead to incomplete methanol removal, and thus methanol accumulation in the reactor effluent from day 142 onwards. This methanol accumulation subsequently induced an increase of the acetogenic activity in the UASB reactor on day 160. On day 165, 77% of the methanol fed to the system was converted to acetate and the Methanosarcina population size had substantially decreased. Inclusion of 0.5 lM Ni (dosed as NiCl 2 ) to the influent from day 165 onwards lead to the recovery of the methanol removal efficiency to 99% without VFA accumulation within 2 days of bioreactor operation.

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“…CODH cleaves the C-C and C-S bonds in the acetyl moiety of acetyl-CoA, oxidizes the carbonyl group to CO 2 , and transfers the methyl group to coenzyme M. MCR catalyzes the enzymatic reduction of methyl-coenzyme M to CH 4 in methanogenesis, which includes a Ni-containing cofactor called F 430 [15,31,40]. Besides, the CODH complex is also involved in the formation of acetate by acetogens from H 2 /CO 2 and methanol [95]. MCR is found exclusively in methanogenic archaea [96].…”

Section: Methanogenesis Stagementioning

confidence: 99%

Biodegradability during Anaerobic Fermentation Process Impacted by Heavy Metals

Tian¹,

Zhang²,

Sanganyado³

2020

New Advances on Fermentation Processes

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In the past decades, biotechnologies for reutilizing the biomass harvested from the metal-contaminated land draw attention to many scientists. Among those technologies, anaerobic fermentation is proven as an efficient conversion process for biowaste reduction with simultaneous recovery of biogas as an energy source. During the process of anaerobic fermentation, the release of metals from the biomass will impact the growth and performance of microorganisms in reactors, which then results the variation of substrate degradation. In this chapter, the impact of metals on the degradation of substrate at different stages of fermentation process, as indicated by variations of lignocelluloses, chemical oxygen demands (COD), volatile fatty acids (VFAs), etc., will be summarized. The objective is to rationalize the relationship between metal presence and substrate degradability and give suggestions for future research on metal-contaminated biomass reutilization.

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Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Cited by 23 publications

References 26 publications

Syntrophic interactions among anode respiring bacteria (ARB) and Non‐ARB in a biofilm anode: electron balances

Syntrophic interactions among anode respiring bacteria (ARB) and Non‐ARB in a biofilm anode: electron balances

Role of nickel in high rate methanol degradation in anaerobic granular sludge bioreactors

Biodegradability during Anaerobic Fermentation Process Impacted by Heavy Metals

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