Growth Kinetics of Embryo- and Organ-Culture Adapted Beaudette Strain of Infectious Bronchitis Virus in Embryonated Chicken Eggs

Yachida, Shunsuke; Aoyama, S; Takahashi, N.; Iritani, Yoshikazu; Katagiri, Ken

doi:10.2307/1589679

Cited by 12 publications

(13 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…( ) 13. For most accurate results, the plaque assay should be repeated three times and the average titer determined.…”

Section: Determine Titer Using the Following Equationmentioning

confidence: 99%

“…Passaging of IBV in either embryonated eggs or primary cell cultures leads to attenuation of the virus in vivo [10][11][12]. The most striking example is the IBV Beaudette strain, which has been passaged hundreds of times in eggs and primary chicken kidney cells [13,14]. Although IBV Beaudette propagates very well on eggs, CK cells and even in Vero cells, the virus is highly attenuated in vivo and vaccination using Beaudette provides little protection against infection with pathogenic strains of IBV [15].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo

Kint

Maier

Jagt

2015

Methods in Molecular Biology

View full text Add to dashboard Cite

show abstract

“…( ) 13. For most accurate results, the plaque assay should be repeated three times and the average titer determined.…”

Section: Determine Titer Using the Following Equationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo

Kint

Maier

Jagt

2015

Methods in Molecular Biology

View full text Add to dashboard Cite

show abstract

“…Tissue samples were homogenized individually and RT-PCR was conducted using primers N(+) and N(−) as described previously [9]. Virus titrations were performed in 9-day-old embryonated chicken SPF eggs via the allantoic cavity route of inoculation, and titers were expressed as 50% (median) embryo infectious doses (EID 50 ) [9,37]. Serial l0-fold dilutions were used for titrations.…”

Section: Virus Titration Recovery and Detectionmentioning

confidence: 99%

Altered pathogenicity, immunogenicity, tissue tropism and 3′-7kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos

Liu

Zhang

Gong

et al. 2009

Vaccine

View full text Add to dashboard Cite

In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of the 3'-7kb region, the CK/CH/LHLJ/04V virus population contained subpopulations with a mixture of genetic mutants. The titers of the virus increased gradually during serial passage, but the replication capacity decreased in chickens. The virus was partially attenuated at passage 40 (P40) and P70, and was fully attenuated at P110. It lost immunogenicity and kidney tropism at P110 and P70, respectively. Amino acid substitutions were found in the 3'-7kb region, primarily in the spike (S) protein. Substitutions in the S1 subunit occurred between P3 and P40 and all subpopulations in a virus passage showed the same substitutions. Other substitutions that occurred between P70 and P110, however, were found only in some subpopulations of the virus passages. A 109-bp deletion in the 3'-UTR was observed in most subpopulations of P70 and P110, and might be related to virus replication, transcription and pathogenicity. The changes described in the 3'-7kb region of the virus are possibly responsible for virus attenuation, immunogenicity decrease and tissue tropism changes; however, we cannot exclude the possibility that other parts of the genome may also be involved in those changes.

show abstract

“…Virus titrations were performed in 9-day-old embryonated chicken SPF eggs via the allantoic cavity route of inoculation (Yachida et al, 1979), and the titers were expressed as 50% (median) embryo infectious doses (EID 50 ). Serial l0-fold dilutions were used for titrations.…”

Section: Growth Ability Of the Ibv Strains In 9-day-old Chicken Embryosmentioning

confidence: 99%

Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3

Liu

Zhang

Chen

et al. 2008

Gene

View full text Add to dashboard Cite

The sequence of a 6.0-kb fragment was compared in the 3'-encoding region of the genome in 27 infectious bronchitis virus (IBV) strains. All these strains have the same S-3-M-5-N gene order, as is the case for other IBVs. However, the sizes of the corresponding open reading frames (ORFs) of some genes varied among the virus strains. Phylogenetic analysis and sequence alignments demonstrated that recombination events had occurred in the origin and evolution of the strains CK/CH/LSD/03I and CK/CH/LLN/98I and the possible recombinant junction sites might be located at the 3c and M genes, respectively. The normal product of ORF 3a is 57 amino acids long, whereas a 43-bp deletion at the 3'-end of the CK/CH/LSD/03I 3a gene was detected, resulting in a frameshift event and C-terminally truncated protein with 47 amino acids. Comparison of the growth ability in embryos and replication and pathogenicity in chickens with IBV carrying the normal 3a gene indicated that this deleted sequence in the 3a gene of CK/CH/LSD/03I was not necessary for viral pathogenesis and replication either in vitro or in vivo. Occurrence of a mutation at the corresponding position of the CK/CH/LLN/98I start codon in the 3a gene led to the absence of ORF 3a in this virus, resulting in a novel genomic organization at the 3'-encoding regions: S-3b, 3c-M-5a, 5b-N. Comparison with other viruses carrying the normal 3a gene revealed that CK/CH/LLN/98I had replication and pathogenicity abilities in vivo similar to those of other IBVs; however, its growth ability in embryos was lower, although the relationship between the lower growth ability and the ORF 3a defect requires further confirmation.

show abstract

Growth Kinetics of Embryo- and Organ-Culture Adapted Beaudette Strain of Infectious Bronchitis Virus in Embryonated Chicken Eggs

Cited by 12 publications

References 5 publications

Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo

Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo

Altered pathogenicity, immunogenicity, tissue tropism and 3′-7kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos

Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3

Contact Info

Product

Resources

About