Growth kinetics of Lactococcus lactis and Lactobacillus casei in liquid culture medium containing as prebiotics inulin or fructose

Abstract

BACKGROUNDPredictive microbiology is a tool that allows us to evaluate the behavior of the concentration of biomass and estimated cells under extrinsic conditions, providing scientific and industrial benefits. In the present study, the growth of L. lactis and L. casei combined with inulin and fructose was modeled using the Gompertz sigmoidal growth functions and plotted using data obtained from batch culture in relation to biomass and cell concentration. expressed estimates. in Ln N (OD 600nm and cells/mL‐1) as a function of time.RESULTSThe results of the kinetic modeling indicated that (T1) A1B1 = L. lactis + Fructose and (T4) A2B2 = L. casei + Inulin presented the best function coefficients and best fits in most cases compared to the rest. , the specific growth rate of the maximum acceleration was from 0.364 to 0.473 h‐1 and 0.100 to 0.129 h‐1, the concentration of bacterial cells (A) was from 0.556 to 0.713 and 0.425 to 0.548 respectively and the time where (μ) occurred with a greater magnitude (L ) fluctuated between 0.854 and 0.802 and when this time in (L) is very fast, it presents values of ≤0.072 to ≤0.092, its coefficient of determination and/or multiple regression (R2) obtained in the two adjustments was 0.97.CONCLUSIONIt was possible to predict the influence of the carbon source on the behavior of maximum growth rates, higher consumption due to nutrient affinity and shorter growth time.This article is protected by copyright. All rights reserved.