Growth kinetics, production, and characterization of extracellular laccases from Pleurotus sajor-caju PS-2001

Bettin, Fernanda; Rosa, Letícia Osório da; Montanari, Queli; Calloni, Raquel; Gaio, Tamara A.; Malvessi, Eloane; Silveira, Maurício Moura da; Dillon, Aldo José Pinheiro

doi:10.1016/j.procbio.2010.12.002

Cited by 43 publications

(43 citation statements)

References 55 publications

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“…The data of work carried out by Bettin et al (2011) also corroborates the results found in the present study, where it was possible to verify a 15-70% discoloration of the dye Reactive Blue 220 when placed in contact with the crude enzyme extract of the fungal strain Pleurotus sajor-caju, suggesting that this strain presents a potential for the discoloration of textile dyes.…”

Section: Resultssupporting

confidence: 89%

“…In another study by Bettin et al (2011), the dye discoloration of chromophore groups such as anthraquinone, azo, and triphenylmethane during the growth of the Pleurotus sajor-caju strain PS-2001 was tested in Petri dishes and the discoloration of the dyes was verified visually, indicating that treatment with this fungal strain could be used in the biotechnological processes.…”

Section: Resultsmentioning

confidence: 99%

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The degradation of methylene blue dye by the strains of Pleurotus sp. with potential applications in bioremediation processes

Maas¹,

Silva²,

Costa³

et al. 2018

Rev. ambiente água

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Fungi have a large capacity to produce enzymes that are capable of degrading compounds. In this regard, soluble dyes are often used as a means of evaluating and selecting strains with potential for biodegradation. This study verified the capacity of the strains of Pleurotus sp. in degrading methylene blue dye, in order to identify the strains with potential for the bioremediation. For this reason, a total of seven strains of Pleurotus sp. were grown in a potato liquid medium with the methylene blue dye at the concentrations of 0.02 g L -1 and 0.04 g L -1 . The results of the absorbances showed that all the isolates were visibly able to reduce the media staining following seven days of growth. However, a reduction of approximately 80% in the staining was observed for the strains named ERY, HI, and SB compared to the control. Thus, it was observed that these strains could be used in the color removal bioprocesses and in the biodegradation of pollutant compounds.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

The degradation of methylene blue dye by the strains of Pleurotus sp. with potential applications in bioremediation processes

Maas¹,

Silva²,

Costa³

et al. 2018

Rev. ambiente água

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“…1 L. Utilizou-se um biorreator com agitação mecânica modelo Biostat  B (volume operacional de 4 L) para a produção enzimática em regime descontínuo, pH 6,5 a 28±1 o C durante 90 horas de processo (Bettin et al, 2011).…”

Section: Methodsunclassified

APLICAÇÃO DE MEDIADORES REDOX NA DESCOLORAÇÃO DE CORANTES UTILIZANDO CALDO ENZIMÁTIDO BRUTO DE Pleurotus sajor-caju PS-2001

Bettin¹,

Cousseau²,

Martins³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -Corantes são estáveis à luz, à temperatura e ao ataque microbiano, representando compostos recalcitrantes. Na presença de mediadores, alguns corantes resistentes à descoloração podem alcançar níveis relevantes de remoção de cor. Diante disso, neste estudo, avaliou-se o efeito da adição de diferentes mediadores sobre a descoloração de 22 corantes pertencentes aos grupos cromóforos antraquinona, azo e trifenilmetano, utilizando lacases produzidas por Pleurotus sajor-caju PS-2001 em processo submerso. As misturas aquosas contendo solução de corante, mediador e caldo enzimático com 30 U.mL -1 de lacases foram mantidas a 35 o C durante 168 horas. De uma forma geral, os percentuais de descoloração obtidos com 2,2′-azino-bis(ácido 3-etilbenzotiazolina-6-sulfônico) sal diamônio (ABTS) como mediador foram inferiores ao controle para treze corantes testados; os melhores resultados foram obtidos com os mediadores hidroxibenzotriazol (HBT) e siringaldazina (SYR). Os corantes trifenilmetano Brilliant Green (65%), Malachite Green (64%) e Bromocresol Green (37%) mostraram os maiores percentuais de descoloração para esta classe. Resultados satisfatórios também foram obtidos com os corantes antraquinona Reactive Blue 220 (48%), Acid Green 28 (46%) e Remazol Brilliant Blue R (37%), assim como para os azo-corantes Levafix Brilliant Red E-4BA (38%), Disperse Blue 79 (37%) e Disperse Orange 30 (33%), com percentuais de descoloração superiores aos controles, preparados na ausência de mediadores.

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“…1C). Bettin et al (2011) observed that suitable pH value for biomass growth was 7.4-7.5. Kalmis et al (2008) suggested that optimum pH for biomass growth is at between 6.5 and 7.0.…”

Section: Selection Of Optimization Rangementioning

confidence: 97%

The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus

Hazuchová¹,

Chmelová²,

Ondrejovič³

2017

Nova Biotechnologica Et Chimica

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The lignocellulolytic enzymes are routinely produced by submerged fermentation using lignocellulosic material, but for more effective production, it would be suitable to precede the production phase on the lignocellulose by propagation phase in the nutrition medium suitable for growth of the fungi. Therefore, the aim of this study was to increase the laccase production by the white-rot fungus Pleurotus ostreatus by two-step cultivation strategy. In the first step, propagation medium was optimized for the maximal biomass growth, the second step included the laccase production by produced fungal biomass in media with the selected lignocellulosic material (pine sawdust, alfalfa steam and corn straw). From our experiments, parameters such as glucose concentration, yeast extract concentration and pH of propagation medium were selected as key factors affecting growth of P. ostreatus. The optimal conditions of propagation medium for maximal fungal growth determined by response surface methodology were: glucose concentration 102.68 g/L, yeast extract concentration 43.65 g/L and pH of propagation medium 7.24. These values were experimentally verified and used statistical model of biomass production prediction was appropriate adjusted. Thus prepared fungal biomass produced in the media with lignocellulose approximately 9-16 times higher concentrations of the laccase in 3 times shorter time than the fungal biomass without propagation phase in optimized propagation medium.

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Growth kinetics, production, and characterization of extracellular laccases from Pleurotus sajor-caju PS-2001

Cited by 43 publications

References 55 publications

The degradation of methylene blue dye by the strains of Pleurotus sp. with potential applications in bioremediation processes

The degradation of methylene blue dye by the strains of Pleurotus sp. with potential applications in bioremediation processes

APLICAÇÃO DE MEDIADORES REDOX NA DESCOLORAÇÃO DE CORANTES UTILIZANDO CALDO ENZIMÁTIDO BRUTO DE Pleurotus sajor-caju PS-2001

The optimization of propagation medium for the increase of laccase production by the white-rot fungus Pleurotus ostreatus

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