Growth model and prediction of oxygen transfer rate for xylitol production from d-xylose by C. guilliermondii

Aguiar, Wilson Bucker; Faria, Luís; Couto, Maria Antonieta Peixoto Gimenes; Araújo, Ofélia de Queiroz Fernandes; Pereira, Nei

doi:10.1016/s1369-703x(02)00040-2

Cited by 37 publications

(33 citation statements)

References 23 publications

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“…C. tropicalis strain LY15 had been extensively studied as a XR producer based upon the xylanase, xylose assimilation test and XR, taking part in xylan to xylitol bioconversion mechanism . XR and xylitol dehydrogenase activity are linked to NADPH and NAD, respectively (Aguiar et al, 2002). The present study shows that there was slight increment in the enzyme production at optimized parameters of different factors.…”

Section: Discussionmentioning

confidence: 99%

Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates

Barathikannan¹,

Khusro²,

Paul³

2016

Afr. J. Microbiol. Res.

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Xylose reductase (XR) is a key enzyme in order to obtain xylitol from xylose. It has vast applications in biotechnology including xylitol production. The present study aimed to estimate and characterize XR from Candida tropicalis strain LY15. C. tropicalis strain LY15 showed xylose utilization ability and xylose reductase activity after 48 h of incubation at pH 6.5, incubation temperature 28°C and rotation speed 140 rpm. It was specific to nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) with an activity of 32.13 IU/ml. Response surface methodology (RSM) was taken into account in order to determine the effect of four main factors, that is, inoculum (1%), hemicellulose waste substrates (HWs) (2%), incubation period (60 h), and RPM (140) on enzyme production. The maximum XR enzyme activity on corn cob substrates (62.80 IU/ml) was found. The xylitol yield (12.08 g/L) attained corn cob media after 60 h of fermentation. Three dimensional response and interaction plot of the quadratic model showed interdependent interaction between the effective variables. Analysis of variance (ANOVA) predicts R 2 value close to 1 which makes the result highly significant (p≤0.0001). These values were higher when compared with the traditional fermentation processes.

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Section: Discussionmentioning

confidence: 99%

Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates

Barathikannan¹,

Khusro²,

Paul³

2016

Afr. J. Microbiol. Res.

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“…An increase in xylitol productivity occurs when the oxygen supply is limited or the maximum oxygen supply is lower than the cell demand (Aguiar et al, 2002). Under anaerobic conditions, yeasts are unable to metabolize D-xylose.…”

Section: Xylitol Productionmentioning

confidence: 99%

“…The medium volume used in the fermentation was 47%, which could be considered aeration with microaerobic tendency. Optimal values of oxygen concentration for xylitol production usually correspond to microaerobic conditions (Aguiar et al, 2002;Salgado et al, 2012). However, the strains grow vigorously at the beginning of fermentation at high initial xylose concentrations and high aeration.…”

Section: Xylitol Productionmentioning

confidence: 99%

Xylose recovery from dilute-acid hydrolysis of oil palm (Elaeis guineensis) empty fruit bunches for xylitol production

Manjarrés-Pinzón¹,

Arias-Zabala²,

Londoño³

et al. 2017

Afr. J. Biotechnol.

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The aim of this study was to evaluate the effect of different process conditions, such as the sulfuric acid concentration, contact time, solid:liquid ratio and particle size, on the xylose recovery and the formation of by-products from oil palm empty fruit bunch (OPEFB) with dilute-acid hydrolysis. Moreover, an adapted and non-adapted strain of the yeast Candida guilliermondii was used to obtain xylitol from the optimal hydrolysate. Xylose, glucose, hydroxymethylfurfural (HMF) and acetic acid in the acid hydrolysate were analyzed with high performance liquid chromatography (HPLC). A BoxBehnken-based design was used to find the combination of factors that maximized the formation of xylose in the hydrolysate optimization process. The fermentation to obtain xylitol from the optimized hydrolysate of OPEFB with adapted and non-adapted C. guilliermondii strains was made in 100 ml Erlenmeyer flasks at 30°C for 96 h at 200 rpm in an incubator shaker. The maximum xylose concentration (32.59 g L -1) was obtained at 121°C, for 30 min, with a 1:8 solid:liquid ratio, 2% acid concentration and particle size of around 4 cm. With the same conditions, the inhibitor concentrations, such as HMF, glucose and acetic acid, were 0.023, 1.033 and 11.078 g L -1, respectively. The optimized conditions were the same as previously described. The higher xylitol productivity (10.3 g L -1) and yield (0.43 g g -1) were obtained by fermentation with non-adapted C. guilliermondii strains from the optimized hydrolysate of OPEFB without the need for detoxification. It is not necessary to make an adaptation of C. guilliermondii in the optimized hydrolysate of OPEFB to produce xylitol.

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“…Xylitol is produced when there is limited oxygen supply, and such conditions lead to an increase in xylitol productivity (Aguiar et al 2002). In the bioreactor fermentations, the availability of oxygen was increased, leading to cell growth.…”

Section: Inoculum Preparation and Xylitol Fermentation In Shake Flaskmentioning

confidence: 99%

“…This situation could be due to the complex medium of sago trunk hydrolysate, which consists of other unknown substances that are consumed by the microorganisms. Aguiar et al (2002) estimated a high value of maximum specific growth rate for Candida guilliermondii as high as 3.259 h -1 using D-xylose as the main substrate. This author explained that high estimated value of affinity constant counterbalanced the decrease in specific growth rate because of the low substrate concentration rather than high cell mass inhibition.…”

Section: Growth Kinetics Of Candida Tropicalismentioning

confidence: 99%

Evaluation of Fermentation Conditions by Candida tropicalis for Xylitol Production from Sago Trunk Cortex

Mohamad¹,

Kamal²,

Abdullah³

et al. 2013

BioResources

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Growth model and prediction of oxygen transfer rate for xylitol production from d-xylose by C. guilliermondii

Cited by 37 publications

References 23 publications

Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates

Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates

Xylose recovery from dilute-acid hydrolysis of oil palm (Elaeis guineensis) empty fruit bunches for xylitol production

Evaluation of Fermentation Conditions by Candida tropicalis for Xylitol Production from Sago Trunk Cortex

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