Growth, Nutrient Uptake, and Yield Promotion of Broccoli by Plant Growth Promoting Rhizobacteria with Manure

Abstract

<jats:p>This study was conducted to investigate the effects of root inoculations with <jats:italic>Bacillus cereus</jats:italic> (N<jats:sub>2</jats:sub>-fixing), <jats:italic>Brevibacillus reuszeri</jats:italic> (P-solubilizing), and <jats:italic>Rhizobium rubi</jats:italic> (both N<jats:sub>2</jats:sub>-fixing and P-solubilizing) on plant growth, nutrient uptake, and yield of broccoli in comparison with manure (control) and mineral fertilizer application under field conditions in 2009 and 2010. Bacterial inoculations with manure compared with control significantly increased yield, plant weight, head diameter, chlorophyll content, nitrogen (N), potassium (K), calcium (Ca), sulfur (S), phosphorus (P), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) content of broccoli. The lowest yield per plant, plant weight, steam diameter, and chlorophyll content were recorded in the control, but the manure with <jats:italic>Bacillus cereus</jats:italic> (BC), <jats:italic>Rhizobium rubi</jats:italic> (RR), and <jats:italic>Brevibacillus reuszeri</jats:italic> (BR) inoculations increased yield 17.0%, 20.2%, and 24.3% and chlorophyll content by 14.7%, 14.0%, and 13.7% over control, respectively. Bacterial inoculations with manure significantly increased uptake of macronutrients and micronutrients by broccoli. In conclusion, seedling inoculation with BR and especially RR may partially substitute costly synthetic fertilizers in broccoli.</jats:p>

Role of microbial inoculation and industrial by‐product phosphogypsum in growth and nutrient uptake of maize (Zea mays L.) grown in calcareous soil

“…N uptake by the inoculated plants amended with PG was 1.9–2.5 times higher than that of the control plants, with the highest N uptake in inoculated plants treated with 10 g PG kg −1 . The enhanced N uptake by inoculated plants with growth‐promoting rhizobacteria applied in the current study could be associated with N fixation or promotion of root surface area, resulting in more nutrient depletion from the soil . Conversely to bacterial inoculations, applying organic manure to uninoculated soil decreased N concentration and uptake by maize shoots, mainly due to reduction of plant growth and the ability to absorb nutrients from the soil by increasing salinity level of the organically amended soil.…”

“…The enhanced N uptake by inoculated plants with growth-promoting rhizobacteria applied in the current study could be associated with N fixation or promotion of root surface area, resulting in more nutrient depletion from the soil. 51 Conversely to bacterial inoculations, applying organic manure to uninoculated soil decreased N concentration and uptake by maize shoots, mainly due to reduction of plant growth and the ability to absorb nutrients from the soil by increasing salinity level of the organically amended soil. However, the treatments that combined both organic manure and microbial inoculation did not significantly change N concentration and its uptake compared with the control plants, indicating the importance of bacterial inoculation to inhibit adverse effects associated with organic manure.…”

A phytobeneficial strainPlanomicrobiumsp. MSSA-10 triggered oxidative stress responsive mechanisms and regulated the growth of pea plants under induced saline environment

“…In an earlier study, PGPR inoculation resulted in salt tolerance by improved plant nutrient balance (Sheng et al 2011) and increased levels of N, P and K were recorded in raddish plants inoculated with Bacillus subtilis and Pseudomonas fluorescens (Mohamed and Gomaa 2012). The increase in nutrient concentration may also ascribe to increase in root surface area and modulated root exudation patterns (Adesemoye et al 2008) as observed in broccoli (Yildirim et al 2011). Likewise, Abou-Shanab et al (2006) reported that a low rhizospheric pH triggered the uptake of cationic nutrients.…”

Serratiasp.CP‐13 augments the growth of cadmium (Cd)‐stressedLinum usitatissimumL. by limited Cd uptake, enhanced nutrient acquisition and antioxidative potential

“…In the present study, the increased nutrient uptake is likely due to increase in root surface area (offering more binding sites for nutrient binding) and subsequently root exudation as reported for broccoli plants (Yildirim et al . ). The root exudation improves the root microbial activity, leading to increased soil‐nutrient solubility and higher influx of nutrients into the plant roots (Ahemad and Kibret ).…”