Growth of Arboviruses in Primary Tissue Culture of Aedes Aegypti Embryos *

Peleg, J.

doi:10.4269/ajtmh.1968.17.219

Cited by 31 publications

(19 citation statements)

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“…gambiae Sua 4.0 cells (Catteruccia et al ., 2000) for AgDef1 , or Ae. aegypti Aag2 cells (Peleg, 1968) for AaDefA4 and AaDefA1 . All full‐length promoter constructs were significantly more active than the promoterless pGL3 vector in dual‐luciferase assays and significantly up‐regulated following immune stimulation with LPS (supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

A novel association between clustered NF‐κB and C/EBP binding sites is required for immune regulation of mosquito Defensin genes

Meredith¹,

Munks²,

Grail³

et al. 2006

Insect Molecular Biology

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A comparative analysis identified key cis-acting regulatory elements responsible for the temporal control of mosquito Defensin gene expression. The promoters of Anopheles gambiae Defensin 1 and two isoforms of Aedes aegypti Defensin A are up-regulated by immune challenge. This stimulated activity depends upon a cluster of three NF-kappaB binding sites and closely associated C/EBP-like motifs, which function as a unit for optimal promoter activity. Binding of NF-kappaB and C/EBP like transcription factors is confirmed by electrophoretic mobility shift assay, including supershifts with antibodies to C/EBP. KappaB-like motifs are abundant within antimicrobial peptide gene promoters and most are very closely associated with putative C/EBP binding sites. This novel association between NF-kappaB and C/EBP binding sites may, therefore, be of widespread significance.

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Section: Resultsmentioning

confidence: 99%

A novel association between clustered NF‐κB and C/EBP binding sites is required for immune regulation of mosquito Defensin genes

Meredith¹,

Munks²,

Grail³

et al. 2006

Insect Molecular Biology

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“…aegypti embryonic cell line [31], Aag-2. Curiously for almost 30 years this lineage was neglected until Gao et al .…”

Section: Discussionmentioning

confidence: 99%

“…aegypti cell lineage of embryonic origin [31] began to be used as a model for studies of mosquito immunity [32-35]. Nevertheless, to our knowledge, no work has been published systematically characterizing the responses of the Aag-2 cell line, against different kinds of pathogens and comparison of the response to the one exhibited by whole mosquitoes, in this way “validating” this cell line as a tool for immune investigations.…”

Section: Introductionmentioning

confidence: 99%

Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies

Barletta

Silva²,

Sorgine

2012

Parasites Vectors

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BackgroundThe understanding of mosquito immune responses can provide valuable tools for development of novel mosquito control strategies. Aiming the study at insect innate immunity, continuous insect cell lines have been established and used as research tools due to the fact that they constitute more homogeneous, sensitive, and reproducible systems than the insects from which they originated. More recently, Aag-2, an Aedes aegypti cell lineage, began to be frequently used as a model for studies of mosquito immunity. Nevertheless, to our knowledge, no study has systematically characterized the responses of Aag-2 cell line against different kinds of pathogens and compared its response to those exhibited by whole mosquitoes. For this reason, in this study we characterized gene expression profiles of the Aag-2 cell line in response to different kinds of immune challenges, such as Gram negative and positive bacteria, fungi and viruses, comparing the obtained results with the ones already described in the literature for whole mosquitoes.MethodsAedes aegypti Aag-2 cells were exposed to different immune stimuli (gram-positive and gram negative heat inactivated bacteria, zymosan or Sindbis virus) for 24 hours and the expression of selected marker genes from toll, IMD and Jak/STAT pathways was analyzed by qPCR. Also, cells were incubated with fluorescent latex beads for evaluation of its phagocytosis capacity.ResultsAag-2 cells were stimulated with two concentrations of heat-killed Gram negative (Enterobacter cloacae) or Gram positive (Micrococcus luteus) bacteria, Zymosan or infected with Sindbis virus and the expression of key genes from the main immune related pathways, Toll, IMD and Jak/STAT, were investigated. Our results suggest that Toll and IMD pathways are activated in response to both Gram positive and negative bacteria and Zymosan in Aag-2 cells, displaying an immune profile similar to those described in the literature for whole mosquitoes. The same stimuli were also capable of activating Jak/STAT pathway in Aag-2 cells. Infection with Sindbis virus led to an up-regulation of the transcription factor STAT but was not able to induce the expression of any other gene from any of the pathways assayed. We also showed that this cell line is able to phagocytose latex beads in culture.ConclusionsOur results characterize the expression profile of Aag-2 cells in response to different immune stimuli and demonstrate that this cell lineage is immune-competent and closely resembles the response described for whole Ae. aegypti mosquitoes. Hence, our findings support the use of Aag-2 as a tool to comprehend Ae. aegypti immune response both at cellular and humoral levels.

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“…To examine the impact of RVFV infection on mosquito cells, we used continuous cell lines derived from Aedes mosquitoes, which are a competent vector for RVFV, the C6/36 and U4.4 cells subcloned from the Singh's original Aedes albopictus cultures (58,59), and the Aag2 cells originally derived from Aedes aegypti by Peleg (60) and further characterized by Lan and Fallon (61). The three mosquito cell lines were infected at a MOI of 2 with the naturally virulent ZH548 strain of RVFV isolated from a human case during the Egyptian outbreak in 1977-1978.…”

Section: Mosquito Cells Are Susceptible To Infection By the Zh548 Strmentioning

confidence: 99%

Dicer-2- and Piwi-Mediated RNA Interference in Rift Valley Fever Virus-Infected Mosquito Cells

Léger

Lara

Jagla

et al. 2013

J Virol

132

150

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Growth of Arboviruses in Primary Tissue Culture of Aedes Aegypti Embryos *

Cited by 31 publications

References 0 publications

A novel association between clustered NF‐κB and C/EBP binding sites is required for immune regulation of mosquito Defensin genes

A novel association between clustered NF‐κB and C/EBP binding sites is required for immune regulation of mosquito Defensin genes

Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies

Dicer-2- and Piwi-Mediated RNA Interference in Rift Valley Fever Virus-Infected Mosquito Cells

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