Growth of avian adeno-associated vims in chicken cells transfected with fowl adenovirus serotype 1 DNA

Bauer, Annette; Monreal, G; Bauer, Hans-Joachim

doi:10.1016/0166-0934(90)90060-s

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…The virus suspension was sonicated after two freeze-thaw cycles, centrifuged through a CsCl cushion and further purified with two CsCl density gradients (density 1.34 g/ml). The resulting virus band was collected, centrifuged and the virus sediment incubated with an SDS-lysis-buffer and a protease to destroy the viral capsid according to a standard protocol (Bauer et al, 1990). The DNA was purified by phenol-chloroform-extraction and the nucleic acid was precipitated in absolute ethanol.…”

Section: Virus Purification and Dna Extractionmentioning

confidence: 99%

Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens

et al. 1998

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Fowl adenoviruses were isolated and characterized from severe cases of hydropericardium syndrome in Ecuador and Pakistan. All were neutralized by antibodies against serotypes 4 and 11. Cross-neutralization tests and restriction enzyme analysis strengthen the classification as serotype 4 strains. The restriction endonucleases used allowed the differentiation among field isolates and reference strains. All field isolates tested induced high embryo mortality. One-day-old specific pathogen free (SPF) chicks were infected with 10 5 plaque-forming units by natural routes to reproduce the disease with plaque purified virus. Whereas no mortality was seen with the reference strain, the mortality with the field isolates was 100%. A reduced mortality occurred with a lower infectious dose. Field isolate K1013 from Ecuador was also highly pathogenic for 1-to 3-week-old SPF chicks after intramuscular inoculation. The main pathological signs were swollen livers, severe hydropericardium and nephritis, reflecting the field situation and underlining the role of FAV4 strains in the aetiology of infectious hydropericardium.

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Section: Virus Purification and Dna Extractionmentioning

confidence: 99%

Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens

et al. 1998

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“…The preparation of virus DNA was carried out according to Bauer et al (1990). In brief, highly purified virus sediment was incubated with an SDS-lysis-buffer and a protease to destroy the capsid.…”

Section: Preparation Of Virus Dnamentioning

confidence: 99%

Identification and characterization of an avian adenovirus isolated from a ‘spiking mortality syndrome’ field outbreak in broilers on the Delmarva Peninsula, USA

1995

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“…To compare the major (1.40) and minor (1.45) component at the level of DNA, e.g., digestion with restriction endonucleases as well as length measurement, AAAV D N A was prepared from the infectious virus particles purified by isopycnic centrifugation in CsC1. Briefly, virions were sedimented at 32,000rpm for 1 h at 4°C in a Beckman SW41 rotor and resuspended in lysis buffer [5]. After incubation at 50 °C for 2h the released D N A was phenolextracted and precipitated with ethanol [18], followed by dissolving in T N E buffer (10mM Tris-HC1, pH 7.5, 100mM NaC1, 1 m M EDTA).…”

mentioning

confidence: 99%

Biological and physicochemical characterization of the major (1.40) and minor (1.45) component of infectious avian adeno-associated virus

Bauer

Schneider

Gelderblom

et al. 1991

Archives of Virology

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Two infectious components with buoyant densities of 1.40 g/cm3 and 1.45 g/cm3, designated as major (1.40) and minor (1.45) component, were detected by banding avian adeno-associated virus (AAAV) isopycnically in CsCl. In metrizamide, however, infectious AAAV banded only as a single peak at a density of 1.32 g/cm3. Biological as well as physicochemical properties of the two AAAV components recovered from CsCl density gradient were described. Concerning the minor (1.45) component, three experimental findings may suggest that the capsid structure of this AAAV population is altered in comparison with that of the major (1.40) component: (i) the sedimentation pattern characterized by an additional peak containing slower-sedimenting noninfectious material (16 S); (ii) the specific infectivity decreased by the 3.5 fold; (iii) the ready disintegration when exposed to gently denaturing conditions.

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Growth of avian adeno-associated vims in chicken cells transfected with fowl adenovirus serotype 1 DNA

Cited by 6 publications

References 11 publications

Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens

Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens

Identification and characterization of an avian adenovirus isolated from a ‘spiking mortality syndrome’ field outbreak in broilers on the Delmarva Peninsula, USA

Biological and physicochemical characterization of the major (1.40) and minor (1.45) component of infectious avian adeno-associated virus

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