Growth of callus and suspension culture cells from cotton varieties (Gossypium hirsutum L.) resistant and susceptible toxanthomonas malvacearum (E. F. Sm.) dows

Ruyack, John; Downing, Michael R.; Chang, Judy S.; Mitchell, Earl D.

doi:10.1007/bf02616144

Cited by 15 publications

(1 citation statement)

References 14 publications

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“…[11]. At 30°C, the optimal temperature for cotton callus growth [17,19], moderate concentrations of Gelrite (0.2% w/v) provide a firmly gelled medium, while agar must be used at high concentrations (1% w/v) for suitable gelling [16]. Gelrite has been used with plant tissue culture of several species, including petunia [9], African violet, Mexican Buckeye, tomato, cotton [21], peach [1] and apple [14].…”

Section: Introductionmentioning

confidence: 99%

Media and gelling agent effect on cotton callus initiation from excised seed hypocotyls

Zimmerman

Robacker

1988

Plant Cell Tiss Organ Cult

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A factorial experiment was performed to develop a medium which would support initiation and proliferation of callus in a diverse group of exotic lines of Gossypium hirsutum.Seed hypocotyls of T1, T25 and T133 were cultured on Linsmaier and Skoog (LS) basal medium (1965) with NAA or 2,4-D tested in combination with BA or kinetin. The best medium from this study was then compared to five published media for support of callus initiation and growth of the varieties Acala 1517-75, Coker 500, Dunn 120, Paymaster 303 and TM 1. Furthermore, the effects of two gelling agents, Difco-Bacto agar and Kelco Gelrite, were investigated with each of the six media. Significantly more callus was initiated on media solidified with Gelrite than with agar. The best callus production occurred on LS medium supplemented with 30gl -~ glucose, 0.1 mgl ~BA and 0.1 mgl ~ 2,4-D.

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Section: Introductionmentioning

confidence: 99%

Media and gelling agent effect on cotton callus initiation from excised seed hypocotyls

Zimmerman

Robacker

1988

Plant Cell Tiss Organ Cult

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Anionic peroxidase production byArnebia euchromacallus

Farhadi¹,

Haghbeen²,

Marefatjo³

et al. 2011

Biotech and App Biochem

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Arnebia euchroma callus, obtained from the root cell culture of an Iranian native specimen, has gained a doubling time of 63 H after regular subculturing on Linsmaier-Skoog (LS) medium containing sugar (50 g/L), 2,4-dichlorophenoxyacetic acid (10(-6) M), and kinetin (10(-5) M) under darkness at 25°C. Despite the observed somaclonal variations, peroxidase production by the A. euchroma calli has been stable over 4 years under the aforementioned conditions. Isoelectric focusing experiments revealed that the partially purified A. euchroma peroxidases (AePoxs) are mainly anionic with pI values of about 5.5 and 6.6. AePox reaches its optimal activity at 55°C and pH 7.5. Results of the various kinetic studies suggest that AePox belongs to the type III plant peroxidases with no activity for the oxidation of 3-indoleacetic acid, but seems to play a role in the lignin biosynthesis and H(2) O(2) regulation during the proliferation of the A. euchroma cells on LS medium. Comparing the biochemical properties of AePox with horseradish peroxidase and in view of the ease of solid cell culture, the A. euchroma callus could be considered as a source of plant peroxidase for some biotechnological applications.

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Isolation and Characterization of Mutant Plant Cell Cultures

Widholm

1983

Plant Cell Culture in Crop Improvement

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Growth of callus and suspension culture cells from cotton varieties (Gossypium hirsutum L.) resistant and susceptible toxanthomonas malvacearum (E. F. Sm.) dows

Cited by 15 publications

References 14 publications

Media and gelling agent effect on cotton callus initiation from excised seed hypocotyls

Media and gelling agent effect on cotton callus initiation from excised seed hypocotyls

Anionic peroxidase production byArnebia euchromacallus

Isolation and Characterization of Mutant Plant Cell Cultures

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