Growth of Candida guilliermondii FTI 20037 on mixed substrate

Gurgel, Patrick V.; Vieira, Délia C.; Furlan, Sandra Aparecida; Mancilha, Ismael Maciel de

doi:10.1590/s1516-89131998000300010

Cited by 1 publication

(1 citation statement)

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“…The major differences in the overall growth rate between wild type and XDH1 defective strains can most probably be attributed to differences in the ability to utilize xylose. However, the parental strain also grew faster than xdhΔ and xdh∆::XR within the first 24-32 h. In this early growth phase only glucose is supposed to be used (shown by (Gurgel et al 1998) and observed by us, data not shown). This might indicate that an intact xylose metabolic pathway seems to be required for realization of the full growth potential.…”

Section: Discussionmentioning

confidence: 76%

Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production

Atzmüller

Ullmann²

2020

AMB Expr

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Meyerozyma guilliermondii, a non-conventional yeast that naturally assimilates xylose, is considered as a candidate for biotechnological production of the sugar alternative xylitol. Because the genes of the xylose metabolism were yet unknown, all efforts published so far to increase the xylitol yield of this yeast are limited to fermentation optimization. Hence, this study aimed to genetically engineer this organism for the first time with the objective to increase xylitol production. Therefore, the previously uncharacterized genes of M. guilliermondii ATCC 6260 encoding for xylose reductase (XR) and xylitol dehydrogenase (XDH) were identified by pathway investigations and sequence similarity analysis. Cloning and overexpression of the putative XR as well as knockout of the putative XDH genes generated strains with about threefold increased xylitol yield. Strains that combined both genetic modifications displayed fivefold increase in overall xylitol yield. Enzymatic activity assays with lysates of XR overexpressing and XDH knockout strains underlined the presumed functions of the respective genes. Furthermore, growth evaluation of the engineered strains on xylose as sole carbon source provides insights into xylose metabolism and its utilization for cell growth.

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Section: Discussionmentioning

confidence: 76%