Growth of cotton fibre and ovules enhanced by IAA and NAA under in vitro conditions

Abstract

An experiment was conducted at the tissue culture laboratory of MGM college of Agricultural Biotechnology, Aurangabad (M.S.) during 2013-14 to evaluate the effects of different concentration levels of auxins viz., NAA and IAA on growth and quality of cotton (Gossipium hirsutum L.) ovules under invitro conditions. The experiment was laid out in Completely Randomized Design, 7 different concentration levels of auxins IAA and NAA. Auxins were tried at the levels of 0, 5, 10 and 15 µM conc. of each in culture of 2 DPA flowers of G. hirsutum L using Beasley and Ting (BT) medium. Cultures were maintained for 21 days and fibre length, fibre weight, ovule length and ovule weight were recorded. Different concentrations of auxins significantly influenced development of cotton ovules under in-vitro conditions. NAA at concentration of 15 µM was found significantly superior over rest of the levels of IAA and NAA in case of fibre length, ovule length and ovule weight whereas IAA concentration of 15 µM was found significantly superior over rest of other concentrations of IAA and NAA for increasing fibre weight.

Evaluation of Selected Characteristics in Industrial Hemp After Phytohormonal Treatment

“…This idea may be supported by a possible correlation with the results of average stem length, where the same pattern is present, although non-significant -the intermediate NAA dosing (10 mg/l) showed practically no difference in stem length from the control group and at the same time it was the only dosing without a significant effect on bark fibre distribution. Increase of fibre yield due to auxin treatment was found under in vitro conditions also in one study with cotton (Chavan et al, 2014). Flavonoids are involved in auxin protection because they prevent degradation activity due to peroxidase activities (Savitsky et al, 1999).…”