Growth of erythroid cells from thawed unseparated cord blood in vitro without exogenous erythropoietin

Loss of the ability to generate large burst‐forming unit‐like megakaryocytic colonies from thawed cord blood in semisolid cultures after short term suspension culture

“…Plating unsorted, thawed CB for cell culture includes several unknowns, such as the presence or loss of significant accessory cells , cytokines released by the CB cell mixture and the presence of negative regulators of megakaryopoiesis. As the erythroid and megakaryocytic cells share a common progenitor , these factors may contribute to the growth of progenitors originating from other lineages of MEP (megakaryocyte–erythrocyte progenitor), such as erythroid cells from unseparated volume‐reduced CB, as recently reported by us . Overall CFU activity was not evaluated in the present study.…”