Growth of Bacillus coagulans in Chemically Defined Media

Abstract

A nutritional study was made of five strains of Bacillus coagulans obtained from various culture collections. These five strains were descendants of two original isolates; three had been derived from one parent culture in years past and the other two were transfers from another parent culture. Therefore, the five cultures should have represented two distinct groups of genetically identical cultures. Three of the strains obtained from one culture collection had become methyl red-negative and sorbitol-negative and had gained abilities to hydrolyze gelatin and ferment arabinose. Nutritional requirements of the five cultures, determined at 37, 45, and 55 C, differed considerably among strains; however, thiamine and biotin were required by all cultures at all temperatures. Aspartic acid was stimulatory at 37 C and was required at 45 C; folic acid, basic amino acids, and certain other nutrilites were required at 55 C. Adenine supplementation was necessary for two strains at 55 C to prevent autolysis; this phenomenon is discussed. The response of these organisms to both serine and the basic amino acids at the three growth temperatures seems especially significant. The media devised for the growth of the five strains of B. coagulans used in this study permit excellent growth at three incubation temperatures.

Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium

“…However, valine at lowered concentrations was found to inhibit growth by up to 77%. The reduced growth due to the reduction of valine can be explained by the fact that this amino acid can stimulate the growth of the thermophilic B. coagulans [ 83 ]. The reduction of alanine leads to an increase in spore yield, since alanine, like other purines, acts as an inducer of germination.…”

Use of Bacillus coagulans as a Dietary Probiotic for the Common Carp, Cyprinus carpio

“…The probiotic, B. coagulans , was originally obtained from common carp aquaculture ponds and examined routinely for purity based on their morphological and biochemical characteristics (Dong and Cai ). The strains were cultured and counted on normal nutrient agar (Difco, Shanghai, China) by spore staining method with the spread plate technique (Marshall and Beers ). Stock culture of the probiotics was stored at −70 C (Forma 702; Thermo, Asheville, NC, USA) in powdered skimmed milk suspension with 25% glycerol prior to use (Cabo et al ).…”

Можливості Корекції Дефіциту Заліза Та Порушень Мікробіоценозу Кишечника В Дітей

“…Примечания: # -достоверность разницы в сравнении с нормативными показателями p < 0,05; *достоверность разницы в сравнении с результатами до лечения p < 0,05. Проведенные исследования подтверждают обоснованность комбинированного применения Bacillus coagulans и витаминов В 9 и В 12 с точки зрения как стимуляции роста и размножения бактерий, так и влияния на системные эффекты (гемопоэз, иммунная система), в связи с тем что витамины В 12 и В 9 являются необходимыми компонентами для скорейшей вегетации спор в толстом кишечнике [10,12,13].…”

“…Взаимодействие этих витаминов заключается также в том, что при длительном применении фолиевой кислоты возможно снижение концентрации в крови витамина В 12 . Поэтому длительное ее применение рекомендуется комбинировать с приемом витамина В 12 [12,13,23].…”