Growth of Keloid‐Producing Fibroblasts in Commercially Available Serum‐Free Media

Hong, Raymond; Lum, Joanne; Koch, R.

doi:10.1016/s0194-5998(99)70239-1

Cited by 19 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After visualization with light microscopy demonstrated cellular release from the flask wall, trypsin soybean inhibitor (Gibco, Grand Island, NY) was placed into each flask in a 1:1 ratio to inactivate the trypsin. UltraCULTURE (Biowhittaker, Walkersville, MD), a serum-free media shown to sustain dermal fibroblast growth for at least 7 days with over 90% viability, 17 was then added to each resulting cell suspension. The cell concentration of each cell suspension was determined using phase contrast microscopy and a hemacytometer.…”

Section: Cell Plating In Serum-free Mediamentioning

confidence: 99%

Effect of Heparin on Production of Basic Fibroblast Growth Factor and Transforming Growth Factor-Beta1 by Human Normal Skin and Hyperplastic Scar Fibroblasts

Fan

Qin

Cai

et al. 2007

Journal of Burn Care & Research

View full text Add to dashboard Cite

Heparin affects both dermal fibroblast proliferation and collagen and may mediate these effects by altering the levels of basic fibroblast growth factor (bFGF) and transforming growth factor-beta1 (TGF-beta1) production as a wound healing modulator. The purpose of this study is to probe the effect of heparin on bFGF and TGF-beta1 production by human normal skin and hyperplastic scar fibroblasts. This research investigates the effect of heparin on bFGF and TGF-beta1 production by human normal skin and hyperplastic scar fibroblasts with exposure to 0, 100, 300, or 600 microg/ml heparin for 24, 48, 72, or 96 hours in a serum-free in vitro model. Levels of bFGF and TGF-beta1 in the supernatants were determined by enzyme-linked immunosorbant assay. All doses of heparin significantly stimulated production of bFGF by normal skin (393% to 1019% increase) and hyperplastic scar fibroblasts (405% to 899% increase) at all time points (P < .05). Heparin (300 and 600 microg/ml) also stimulated TGF-beta1 production by normal skin (26% to 83%) and hyperplastic scar fibroblasts (63% to 85%) with statistical significance (P < .05) at various time points. These effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastic scar formation and wound healing in vivo.

show abstract

Section: Cell Plating In Serum-free Mediamentioning

confidence: 99%

Effect of Heparin on Production of Basic Fibroblast Growth Factor and Transforming Growth Factor-Beta1 by Human Normal Skin and Hyperplastic Scar Fibroblasts

Fan

Qin

Cai

et al. 2007

Journal of Burn Care & Research

View full text Add to dashboard Cite

show abstract

“…UltraCULTURE (Biowhittaker, Walkersville, MD), a serum-free media shown to sustain fibroblast growth for at least 7 days with more than 90% viability, 17 was then added to each resulting cell suspension. At the time of experimentation, confluent fibroblasts were released from the culture flasks with 0.05% trypsin.…”

Section: Cell Plating In Serum-free Mediamentioning

confidence: 99%

Triamcinolone Stimulates bFGF Production and Inhibits TGF-β1 Production by Human Dermal Fibroblasts

Carroll

Hanasono

Mikulec

et al. 2002

Dermatologic Surgery

Self Cite

View full text Add to dashboard Cite

background. Triamcinolone acetonide has been shown to decrease both cellular proliferation and collagen production by dermal fibroblasts. An alteration of cytokine levels may mediate these effects. objective. To delineate the effect of triamcinolone acetonide on both cellular proliferation and the production of basic fibroblast growth factor (bFGF) and transforming growth factor ␤ 1 (TGF-␤ 1) by human fibroblasts grown in a serum-free in vitro model. methods. Human normal and keloid dermal fibroblasts were propagated in a serum-free in vitro model with exposure to 0, 5, 10, or 20 m triamcinolone acetonide for 0, 24, 72, or 96 hours. Cell counts were determined by phase contrast micros-copy. Levels of bFGF and TGF-␤ 1 in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA). results. In our study, 20 m triamcinolone acetonide caused statistically significant increases in the peak levels of bFGF for normal and keloid fibroblast cell lines ( P Ͻ 0.05). It also caused statistically significant decreases in the level of TGF-␤ 1 for normal and keloid fibroblast cell lines. For the keloid fibroblasts, 10 m triamcinolone acetonide also caused a statistically significant decrease in the level of TGF-␤ 1. conclusion. We conclude from these results that triamcinolone acetonide increases the production of bFGF and decreases production of TGF-␤ 1 by human dermal fibroblasts. L. A. CARROLL, MD, M. M. HANASONO, MD, A. A. MIKULEC, MD, M. KITA, AND R. J. KOCH, MD HAVE INDICATED NO SIGNIFICANT INTEREST WITH COMMERCIAL SUPPORTERS.

show abstract

“…bFGF is a member of the fibroblast growth factor family, which is known for its regulatory, mitogenic, and angiogenic abilities [17]. In addition, bFGF can increase cell survival rate and stabilize cell phenotype [18]. In the present study, the mRNA expressions of TGF-β1 and bFGF were significantly lower in the siRNA-miR-184 group than in the control and scramble siRNA groups.…”

Section: Discussionmentioning

confidence: 44%

MicroRNA-184 Promotes Proliferation and Inhibits Apoptosis in HaCaT Cells: An In Vitro Study

Cao

Chen

et al. 2016

Med Sci Monit

View full text Add to dashboard Cite

BackgroundThis study aimed to investigate the role of miR-184 in the proliferation and apoptosis of keratinocyte (HaCaT cells).Material/MethodsHaCaT cells were cultured in a growth medium. The miR-184 was transfected with siRNA, then cell viability and apoptosis were assayed by MTT and flow cytometry, respectively. The colony-forming efficacy of HaCaT cells were detected as well. mRNA expressions of basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)-β1 were measured with RT-PCR. The expressions of apoptosis-related proteins caspase-3 and Bcl-x in HaCaT cells were determined by Western blot.ResultsAfter miR-184 was transfected with siRNA, cell viability and colony forming ability decreased significantly, and apoptosis was significantly increased. The expressions of growth factors TGF-β1 and bFGF mRNAs, as well as apoptosis-related proteins Bcl-x, in HaCaT cells declined significantly after miR-184 was transfected with siRNA. In addition, the expression of pro-apoptotic protein caspase-3 increased significantly.ConclusionsOur results suggest distinct roles of miR-184 during the growth, proliferation, and apoptosis of keratinocytes.

show abstract

Growth of Keloid‐Producing Fibroblasts in Commercially Available Serum‐Free Media

Cited by 19 publications

References 12 publications

Effect of Heparin on Production of Basic Fibroblast Growth Factor and Transforming Growth Factor-Beta1 by Human Normal Skin and Hyperplastic Scar Fibroblasts

Effect of Heparin on Production of Basic Fibroblast Growth Factor and Transforming Growth Factor-Beta1 by Human Normal Skin and Hyperplastic Scar Fibroblasts

Triamcinolone Stimulates bFGF Production and Inhibits TGF-β1 Production by Human Dermal Fibroblasts

MicroRNA-184 Promotes Proliferation and Inhibits Apoptosis in HaCaT Cells: An In Vitro Study

Contact Info

Product

Resources

About