Growth of Listeria monocytogenes at 21°C in Biofilms with Micro-organisms Isolated from Meat and Dairy Processing Environments

Jeong, Dong Kwan; Frank, Joseph F.

doi:10.1006/fstl.1994.1087

Cited by 48 publications

(15 citation statements)

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“…Pseudomonads are among the better-studied microorganisms with respect to phenotypic changes taking place throughout the process of biofilm formation and the genetic determinants involved [50-53]. These are commonly found in the food-processing environment [54,55] and have been previously documented as good producers of extracellular polymeric substances, including polysaccharides, nucleic acids, and proteins [56-58], making them ideal organisms with which to investigate the growth of L. monocytogenes within multispecies biofilms. Notably, P. putida bacteria are capable of adhering to various food contact surfaces and form strong biofilms [5,53,59-62].…”

Section: Introductionmentioning

confidence: 99%

Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride

Chorianopoulos²,

et al. 2013

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Biofilm formation is a phenomenon occurring almost wherever microorganisms and surfaces exist in close proximity. This study aimed to evaluate the possible influence of bacterial interactions on the ability of Listeria monocytogenes and Pseudomonas putida to develop a dual-species biofilm community on stainless steel (SS), as well as on the subsequent resistance of their sessile cells to benzalkonium chloride (BC) used in inadequate (sub-lethal) concentration (50 ppm). The possible progressive adaptability of mixed-culture biofilms to BC was also investigated. To accomplish these, 3 strains per species were left to develop mixed-culture biofilms on SS coupons, incubated in daily renewable growth medium for a total period of 10 days, under either mono- or dual-species conditions. Each day, biofilm cells were exposed to disinfection treatment. Results revealed that the simultaneous presence of L. monocytogenes strongly increased the resistance of P. putida biofilm cells to BC, while culture conditions (mono-/dual-species) did not seem to significantly influence the resistance of L. monocytogenes biofilm cells. BC mainly killed L. monocytogenes cells when this was applied against the dual-species sessile community during the whole incubation period, despite the fact that from the 2nd day this community was mainly composed (>90%) of P. putida cells. No obvious adaptation to BC was observed in either L. monocytogenes or P. putida biofilm cells. Pulsed field gel electrophoresis (PFGE) analysis showed that the different strains behaved differently with regard to biofilm formation and antimicrobial resistance. Such knowledge on the physiological behavior of mixed-culture biofilms could provide the information necessary to control their formation.

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Section: Introductionmentioning

confidence: 99%

Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride

Chorianopoulos²,

et al. 2013

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“…This microorganism adheres and forms biofilms on numerous surfaces (15,27), generally in association with other microorganisms such as Pseudomonas spp. Biofilms may represent an important source of contamination for materials or foodstuffs coming into contact with them (20,21,29). Several reports have shown that sessile bacterial cells are more resistant to environmental changes and cleaning or disinfection treatments (23).…”

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confidence: 99%

Listeria monocytogenes LO28: Surface Physicochemical Properties and Ability To Form Biofilms at Different Temperatures and Growth Phases

Chavant¹,

Martinie

Meylheuc³

et al. 2002

Appl Environ Microbiol

290

175

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The surface physicochemical properties of Listeria monocytogenes LO28 under different conditions (temperature and growth phase) were determined by use of microelectrophoresis and microbial adhesion to solvents. The effect of these parameters on adhesion and biofilm formation by L. monocytogenes LO28 on hydrophilic (stainless steel) and hydrophobic (polytetrafluoroethylene [PTFE]) surfaces was assessed. The bacterial cells were always negatively charged and possessed hydrophilic surface properties, which were negatively correlated with growth temperature. The colonization of the two surfaces, monitored by scanning electron microscopy, epifluorescence microscopy, and cell enumeration, showed that the strain had a great capacity to colonize both surfaces whatever the incubation temperature. However, biofilm formation was faster on the hydrophilic substratum. After 5 days at 37 or 20°C, the biofilm structure was composed of aggregates with a threedimensional shape, but significant detachment took place on PTFE at 37°C. At 8°C, only a bacterial monolayer was visible on stainless steel, while no growth was observed on PTFE. The growth phase of bacteria used to inoculate surfaces had a significant effect only in some cases during the first steps of biofilm formation. The surface physicochemical properties of the strain are correlated with adhesion and surface colonization.

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“…Although L monocytogenes is generally regarded as a poor biofilm former, the development of multispecies biofilms in food-processing facilities (3,22) is thought to be a major vehicle for the amplification and subsequent contamination of food products (44). This report describes the impact of phenotypic variation on the formation of pure-species biofilms on a stainless steel surface by a virulent smooth isolate of L. monocytogenes and the selection of a revertant smooth morphotype in broth culture.…”

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confidence: 99%

Morphotypic Conversion inListeria monocytogenesBiofilm Formation: Biological Significance of Rough Colony Isolates

Monk¹,

Cook²,

Monk³

et al. 2004

Appl Environ Microbiol

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Adherence to a stainless steel surface selected isolates of Listeria monocytogenes with enhanced surface colonization abilities and a change in phenotype from the common smooth colony morphology to a succession of rough colony morphotypes. Growth in broth culture of the best-adapted, surface-colonizing rough colony morphotype gave a smooth colony revertant. Comparative analysis revealed that the smooth and rough variants had similar phenotypic and biochemical characteristics (e.g., identical growth rates and tolerances to antibiotics and environmental stressors). Rough colony isolates, however, failed to coordinate motility or induce autolysis. The defect in autolysis of rough colony isolates, which involved impaired cellular localization of several peptidoglycan-degrading enzymes, including cell wall hydrolase A (CwhA), suggested a link to a secretory pathway defect. The genetic basis for the impairment was studied at the level of the accessory secretory pathway component SecA2. DNA sequencing of the secA2 gene in smooth and rough colony isolates found no mutations in the coding or promoter regions. Analysis of SecA2 expression with an integrated secA2-FLAG tag construct found the protein to be upregulated in the rough and revertant backgrounds compared to the parental smooth colony isolate. A compensatory mechanism involving the SecA2 secretion pathway components is postulated to control smooth to rough interconversion of L. monocytogenes. Such phenotypic variation may enhance the ability of this opportunistic pathogen to colonize environments as diverse as processing surfaces, food products, and animal hosts.Changes in bacterial colony morphology often accompany microbial adaptation to new environments and ecological niches (12,19,32,34). Understanding the molecular basis of morphotypic change during biofilm formation on abiotic surfaces and the subsequent seeding and adaptation to planktonic growth have important implications for the food industry and medicine. For example, the fouling of conveyor belts and prosthetic devices can lead to contamination of food products and the spread of infection, respectively (16,36). Experimental models that are amenable to molecular methods are needed so that these problems can be circumvented. The readily cultured, intracellular, food-borne pathogen Listeria monocytogenes provides a practical model to explore morphotypic variation and its role in microbial adaptation.Variant rough colony morphotypes were first described within a decade of the discovery of smooth-colony-forming L. monocytogenes (17). The rough colony morphotype was thought to occur spontaneously and irreversibly at low frequency during prolonged culture in the laboratory. Apart from obvious physical differences, such as the absence of a bluegreen sheen upon Henry illumination and impaired cell separation that gave chaining cells without coordinated motility, the fermentative and biochemical profiles of rough and smooth colonies were considered identical (16,17,43).Characterization of the L. monocytogenes s...

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Growth of Listeria monocytogenes at 21°C in Biofilms with Micro-organisms Isolated from Meat and Dairy Processing Environments

Cited by 48 publications

References 0 publications

Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride

Co-Culture with Listeria monocytogenes within a Dual-Species Biofilm Community Strongly Increases Resistance of Pseudomonas putida to Benzalkonium Chloride

Listeria monocytogenes LO28: Surface Physicochemical Properties and Ability To Form Biofilms at Different Temperatures and Growth Phases

Morphotypic Conversion inListeria monocytogenesBiofilm Formation: Biological Significance of Rough Colony Isolates

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