1971
DOI: 10.1128/jb.105.2.646-656.1971
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Growth of Streptococcal Protoplasts and L-Colonies on Membrane Filters

Abstract: Membrane filters (Millipore Corp.; pore sizes 1.2 to 0.22 Mm) were placed on the surface of L-phase growth medium solidified with agar. The filter and the surrounding medium were inoculated with either protoplasts or stable broth-grown Lphase variants obtained from Streptococcus faecium strain F24. The L-phase inoculum gave rise to viable L-colonies on the filters and on the medium, whereas protoplasts gave colony formation only on the medium. However, when the Millipore filters were covered by a layer of soli… Show more

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Cited by 5 publications
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“…The origin of small bodies present in considerable numbers both intracellularly and extracellularly throughout the 7-day incubation period is unknown. If these small bodies are related to the infecting organisms, their capacity to initiate or perpetuate infection is questionable, as conflicting results have been obtained regarding viability of such structures in L-form cultures of other bacterial species (3,4,6,21…”
mentioning
confidence: 99%
“…The origin of small bodies present in considerable numbers both intracellularly and extracellularly throughout the 7-day incubation period is unknown. If these small bodies are related to the infecting organisms, their capacity to initiate or perpetuate infection is questionable, as conflicting results have been obtained regarding viability of such structures in L-form cultures of other bacterial species (3,4,6,21…”
mentioning
confidence: 99%