Growth of Streptococcus mutans in a chemostat

Ellwood, D. C.; Hunter, J. R.; Longyear, V. M. C.

doi:10.1016/0003-9969(74)90134-4

Cited by 43 publications

(18 citation statements)

References 12 publications

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“…Conversely, little lactate accumulated during growth with 6 or 13 g glucose 1-1 in the feed, and ethanol and acetate were the major fermentation products (Table 1). The fermentation products and the effects of increasing the glucose concentration on the bacterial yield during growth with 6 or 36 g 1-1 were entirely consistent with data reported previously for cultures in which glucose was growth-limiting or in excess (Carlsson & Griffith, 1974;Ellwood et al, 1974), so these concentrations of fermentable carbohydrate were used subsequently. Ecfect of carbon source on glucosyl-and fructosyltransferase synthesis Glucose and fructose are products of fructosyl-and glucosyltransferase activities when sucrose is the substrate, so the effects on enzyme synthesis of the availability of each of these compounds during growth were investigated.…”

Section: Growth-limiting Nutrient In Continuous Cultures With Difleresupporting

confidence: 68%

Regulation of Glucosyl- and Fructosyltransferase Synthesis by Continuous Cultures of Streptococcus mutans

Wenham

Hennessey

Cole

1979

Journal of General Microbiology

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117Streptococcus mutans strain Ingbritt, and its derivative B7 which had been passaged through monkeys, have been used to investigate how the synthesis of extracellular glucosyl-and fructosyltransferases is regulated. The most active enzyme from carbon-limited continuous cultures was a fructosyltransferase ; enzymes catalysing the formation of water-insoluble glucans from sucrose were relatively inactive. Dextransucrase (EC 2.4.1 .5), which catalyses soluble glucan synthesis, was most active in the supernatant fluid from cultures grown with excess glucose, fructose or sucrose, but full activity was detected only when the enzyme was incubated with both sucrose and dextran. Little dextransucrase activity was detected in carbon-limited cultures. It is concluded that glucosyl-and fructosyltransferases are constitutive enzymes in that they are synthesized at similar rates during growth with an excess of the substrate or of the products of the reactions which they catalyse. Although the Ingbritt strain was originally isolated from a carious lesion, it is now a poor source of glucosyltransferase activity. Glucosyltransferases were extremely active in cultures of a recent clinical isolate, strain 3209, and were apparently induced during growth with excess glucose.

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Section: Growth-limiting Nutrient In Continuous Cultures With Difleresupporting

confidence: 68%

Regulation of Glucosyl- and Fructosyltransferase Synthesis by Continuous Cultures of Streptococcus mutans

Wenham

Hennessey

Cole

1979

Journal of General Microbiology

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“…Streptococcus sanguis was chosen because it adheres to glass and is representative of organisms that are often present in the bacterial deposits found on teeth (Gibbons & van Houte, 1973). The influence of growth rate on the rate at which cells accumulated on the glass surface was also observed since it has been suggested that growth rate has a considerable influence on bacterial adhesion (Ellwood et al, 1974).…”

Section: Introductionmentioning

confidence: 99%

The Deposition of Streptococcus sanguis NCTC 7868 from a Flowing Suspension

Rutter¹,

Leech²

1980

Microbiology

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Measurements were made of the rates at which continuously cultured Streptococcus sanguis NCTC 7868 cells accumulated on the inside surfaces of narrow glass capillaries from suspensions of the bacteria flowing down the capillaries at different velocities. Initially, the rate of accumulation of bacteria on the clean walls of the capillary was rapid. The deposition rate decreased with time, however, resulting in a saturation coverage of the glass surface which was considerably less than a monolayer. Multilayer coverage of the tube surface by bacteria was only achieved when fresh nutrient was pumped over deposited cells. This was attributed to cell growth. Although theoretical considerations of the deposition of small particles on to the walls of a tube suggest that the initial deposition rate should increase with flow rate, this was not the case with cells grown at dilution rates of 0.2 and 0.5 h-1. It is suggested that this can be explained by a polymer bridging mechanism of attachment.

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“…Our observations further indicate that while organisms will grow under glucose limitation at appropriate pH without Tween 80, their morphology is frequently aberrant, with the production of filamentous and branched structures. Studies with Actinomyces viscosus in continuous culture have also shown that morphology is influenced by the growth conditions (van der Hoeven, 1974; Ellwood et al, 1977).…”

Section: Discussionmentioning

confidence: 96%

Effect of Tween 80 on the Morphology and Physiology of Lactobacillus salivarius Strain IV CL-37 Grown in a Chemostat under Glucose Limitation

et al. 1980

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The effect of Tween 80 on Lactobacillus salivarius strain IV CL-37 growing in a chemostat under various conditions was investigated. The organisms could grow under glucose limitation in the absence of Tween 80 at pH 6.0 or lower anaerobically but not aerobically. Aerobic growth under glucose limitation and in the presence of Tween 80 occurred in complete MRS medium but not in the dialysable fraction of MRS medium. The morphology of cells differed from coccal to filamentous and branched structures according to the growth condition. The possible effect of Tween 80 on membrane components was examined by estimating the cellular and extracellular lipoteichoic acid contents. In both batch and continuous culture the amounts of cellular lipoteichoic acid were inversely related to the amount of Tween 80 whereas the amounts of extracellular lipoteichoic acid were influenced by other factors in addition to Tween 80.

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Growth of Streptococcus mutans in a chemostat

Cited by 43 publications

References 12 publications

Regulation of Glucosyl- and Fructosyltransferase Synthesis by Continuous Cultures of Streptococcus mutans

Regulation of Glucosyl- and Fructosyltransferase Synthesis by Continuous Cultures of Streptococcus mutans

The Deposition of Streptococcus sanguis NCTC 7868 from a Flowing Suspension

Effect of Tween 80 on the Morphology and Physiology of Lactobacillus salivarius Strain IV CL-37 Grown in a Chemostat under Glucose Limitation

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