Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required when iron is limited.

Bauer, Carolyn M.; Scappino, Lori; Haselkorn, Robert

doi:10.1073/pnas.90.19.8812

Cited by 77 publications

(71 citation statements)

References 22 publications

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“…In either of these cases, STRR sequences would reflect unexpected genome plasticity. A similar observation was made in the case of the variation in STRR sequences found in the nif] gene (Bauer et a/., 1993).…”

Section: Discussion Strr Sequences In Cyanobacteriasupporting

confidence: 49%

Characterization of a nitrogen-fixation (nif) gene cluster from Anabaena azollae 1a shows that closely related cyanobacteria have highly variable but structured intergenic regions

Jackman

Mulligan

1995

Microbiology

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Section: Discussion Strr Sequences In Cyanobacteriasupporting

confidence: 49%

Characterization of a nitrogen-fixation (nif) gene cluster from Anabaena azollae 1a shows that closely related cyanobacteria have highly variable but structured intergenic regions

Jackman

Mulligan

1995

Microbiology

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“…It is similar to NifJ, a well characterized Por present in nitrogen fixing photosynthetic bacteria (59% identity to Q06879 from Nostoc sp. PCC 7120), where this enzyme has a role in providing electrons to ferredoxin or flavodoxin, the electron donors for nitrogenase [29].…”

Section: Pyruvate Oxidation Redundancymentioning

confidence: 99%

Computational and Experimental Analysis of Redundancy in the Central Metabolism of Geobacter sulfurreducens

Segura¹,

Mahadevan²,

Juárez³

et al. 2005

PLoS Comput Biol

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Previous model-based analysis of the metabolic network of Geobacter sulfurreducens suggested the existence of several redundant pathways. Here, we identified eight sets of redundant pathways that included redundancy for the assimilation of acetate, and for the conversion of pyruvate into acetyl-CoA. These equivalent pathways and two other sub-optimal pathways were studied using 5 single-gene deletion mutants in those pathways for the evaluation of the predictive capacity of the model. The growth phenotypes of these mutants were studied under 12 different conditions of electron donor and acceptor availability. The comparison of the model predictions with the resulting experimental phenotypes indicated that pyruvate ferredoxin oxidoreductase is the only activity able to convert pyruvate into acetylCoA. However, the results and the modeling showed that the two acetate activation pathways present are not only active, but needed due to the additional role of the acetyl-CoA transferase in the TCA cycle, probably reflecting the adaptation of these bacteria to acetate utilization. In other cases, the data reconciliation suggested additional capacity constraints that were confirmed with biochemical assays. The results demonstrate the need to experimentally verify the activity of key enzymes when developing in silico models of microbial physiology based on sequence-based reconstruction of metabolic networks.

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“…In addition, upstream of the Anabaena icd gene, there are six imperfect copies of a 7-bp repeating sequence that has the consensus sequence CCCCAAT (45). Heptanucleotides with different consensus sequences have been detected near other genes in heterocystous cyanobacteria (2,15,21,43,58). The fact that these heptamer repeats are in some cases transcribed and translated, along with the lack of clear promoter sequences in the icd 5Ј flanking region and the size of the transcript detected, could indicate that these repeats are also transcribed, the promoter of icd being far away from this region.…”

Section: Resultsmentioning

confidence: 99%

The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria

1996

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Cyanobacteria are photosynthetic prokaryotes that have an incomplete tricarboxylic acid cycle, lacking ␣-ketoglutarate dehydrogenase and succinyl-coenzyme A synthetase activities (46, 54). The NADP ϩ -isocitrate dehydrogenase (IDH) (EC 1.1.1.42) reaction represents a terminal step in carbon flow; thus, the role of this enzyme is the provision of biosynthetic precursors rather than energy production. The ␣-ketoglutarate produced in the NADP ϩ -IDH reaction is required for ammonium assimilation through the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway (38) and is a key metabolite in the linking of nitrogen and carbon metabolism.IDHs have been purified and characterized from a variety of sources (8); most bacteria have only an NADP ϩ

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Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required when iron is limited.

Abstract: The nifJ gene of KkebsieUa pneumoniae encodes an oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin, which reduces nitrogenase. The

Cited by 77 publications

References 22 publications

Characterization of a nitrogen-fixation (nif) gene cluster from Anabaena azollae 1a shows that closely related cyanobacteria have highly variable but structured intergenic regions

Characterization of a nitrogen-fixation (nif) gene cluster from Anabaena azollae 1a shows that closely related cyanobacteria have highly variable but structured intergenic regions

Computational and Experimental Analysis of Redundancy in the Central Metabolism of Geobacter sulfurreducens

The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria

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