Growth of the Desulfomicrobium sp. strains, their sulfate- and lactate usage, production of sulfide and acetate by the strains isolated from the human large intestine

Abstract

The aim and background of the study were to investigate bacterial growth of the Desulfomicrobium sp. strains from human large intestine, and to study the processes usage of sulfate-and lactate, production of sulfide and acetate by the obtained bacteria as well as to carry out cluster and correlation analysis of these processes. Microbiology methods of the study for bacterial strains cultivation and photometric methods for determination of bacterial biomass and hydrogen sulfide concentration were used, sulfate ions concentration was determined by turbidymetric method, lactate concentration was carried out by lactate dehydrogenase. Acetate ions accumulation by the strains was determined by titration. Using the experimental data, the methods of statistical analysis have been also used. The highest biomass (3.34 g/l) has been accumulated by the Desulfomicrobium sp. strain Rod-9 on the sixth day of cultivation, in comparison to the other strains. Studied strains of the Desulfomicrobium sp. actively reduced the sulfate ions and produced hydrogen sulfide. The highest concentration of hydrogen sulfide (3.14 mM) was produced by the SRB Rod-9 strain on the eighth day of cultivation; while, the strain used about 98% of the sulfate compared with its initial concentration. The strains used lactate fully on the sixth day of cultivation and produced acetate. Clustering of the parameters of the bacterial growth, the sulfate and lactate usage as well as hydrogen sulfide and acetate production by the various Desulfomicrobium sp. strains has been carried out. The correlation coefficients between the parameters have been determined.

Harnessing sulfate-reducing bacteria with plants growing to revitalize metal-tainted coal mine soils in Midwest China: metal sequestration performance, ecological networking interaction, and functional enzymatic prediction

“…Such scenario implies a richer diversity in the Desulfovibrio population, albeit with a lesser collective abundance compared to Desulfobulbus . Conversely, genera such as Desulfomicrobium ( Kushkevych, 2014 ) and Desulfobacter ( Fang et al, 2019 ) were identified to a lesser extent, indicating their sparse representation in the consortium. This distribution underscores a competitive advantage for the Desulfovibrio and Desulfobulbus genera, facilitating their survival and nutrient competition in the sewage sludge origin, thereby delineating their significant ecological dominance in environments favorable to SRB.…”

Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms

“…clone MFC-2-L19, Desulfobulbus propionicus strain ). The sulfate-reducing bacteria (SRB) are a heterogeneous group of microorganisms which use sulfate as an electron acceptor in the process of dissimilatory sulfate reduction [35,36]. The final product of this process is hydrogen sulfide [6,20,22,37].…”

Dissimilatory sulfate reduction in the intestinal sulfate-reducing bacteria

“…Intestinal sulfate-reducing bacteria and the bowel diseases. Sulfate-reducing bacteria Desulfotomaculum, Desulfobulbus, Desulfomicrobium, Desulfomonas, and Desulfovibrio genera belong to the intestinal microbiocenosis in humans and animals [1,22,23,24,46]. The knowledge on the interaction of SRB with other microorganisms in intestines is not sufficient.…”