Growth of the lower spleen pole remaining after subtotal splenectomy in rats

Purpose To evaluate the viability of the upper (UP) and lower pole (LP) of the spleen from a macro and microscopic point of view, after subtotal splenectomy with preservation (SSP) of the UP and the LP. Methods Seventeen male Wistar rats, two months old, were submitted to SSPUP and SSPLP and 5 to simulated operation (SG). After 80 days, the rats were euthanized, and the remaining LP and UP and intact spleens were evaluated macroscopically and microscopically. Results Two rats died during the operation. Macroscopic analysis showed that in 15 LP, one of them was not viable and in 15 UP and in 5 spleens in the SG, all were viable. In the statistical analysis, there was no difference in relation to viability. The LP and UP analyzed showed variation. As for the length, the UP increased significantly; however, in relation to the width, there was a significant increase in the LP in relation to the UP. In addition, the weight of the UP was significantly greater than that of the LP. Microscopic analysis attested viability of the splenic remnants. Conclusion There was no significant difference regarding the viability of UP and LP, in macroscopy and microscopy.

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“…The same occurred with the width and thickness. These results go against those of a study that shows that the lower pole grows over time 16 .…”

Section: Discussioncontrasting

confidence: 94%

Macro and microscopic comparison of the upper pole of the spleen with the lower pole in partial splenectomy

et al. 2020

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“…The average inferior pole percentage was greater on postoperative day 80 than on day 10. This suggests that the splenic remnant grew over time, as described by Paulo et al 32 In another partial splenectomy model, growth was also seen in the inferior pole 33 . This is a promising observation, as it suggests that splenic function can improve, and was demonstrated in previous work 5 .…”

Section: Discussionsupporting

confidence: 58%

Subtotal laparoscopic splenectomy in rats with preservation of the inferior pole

Silva

Paulo

2011

Acta Cir. Bras.

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Purpose:To evaluate the feasibility and safety of subtotal splenectomy by laparoscopy with inferior pole preservation and to determine the viability of the splenic remnant. Methods: Twenty male Wistar rats weighing between 365 g and 474 g (mean 417.92 ± 36.15g SD) were operated and randomly assigned to two groups : Group 1 rats were killed on postoperative day 10 (n = 10), and Group 2 on the postoperative day 80 (n = 10). Both the inferior splenic pole and the superior part of the spleen, which was used as a control to measure inferior pole viability, were weighed and morphologically examined. Results: The technique was feasible in all cases. There were two postoperative deaths on immediate postoperative and one not well defined. The average weight percentage of the inferior pole, measured indirectly on the 10th day, was 53.67% ± 11.59% and on the 80th day was 62.69% ± 6.89%. The inferior pole was necrotic, with abscess formation in one case (5.9%) and appeared normal in all other cases. Microscopy showed features that were compatible with normality. Conclusions: Subtotal splenectomy with preservation of the inferior pole by laparoscopy was feasible and safety. The lower splenic pole was viable in 94.1% of animals. Key words: Spleen. Splenectomy. Laparoscopy. Rats. RESUMOObjetivo: Avaliar a exequibilidade e a segurança da esplenectomia subtotal com preservação do polo inferior do baço por videolaparoscopia, e a viabilidade do referido polo. Métodos: 20 ratos Wistar, machos, pesando entre 365g e 474g (MA 417,92 ± 36,15g) foram operados e distribuídos em dois grupos de acordo com a época da retirada do polo inferior para estudo: grupo 1 do 10º dia, (n= 10), grupo 2, do 80º dia (n=10). A porção superior do baço que serviu de controle da viabilidade do pólo inferior e esse remanescente, retirados no término do experimento, foram medidos, pesados e enviados para exame morfológico. Resultados: A técnica foi exeqüível em todos os casos. O peso médio percentual do polo inferior avaliado de forma indireta no 10º dia foi 53,67% ± 11,59%, e no 80º dia 62,69% ± 6,89%. Houve dois óbitos após a cirurgia, provavelmente por mal condução da anestesia e/ou do pneumoperitônio, e um tardiamente por causa desconhecida. O exame macro e microscópico do polo inferior nos 17 animais sobreviventes e no que faleceu tardiamente mostrou necrose em um caso (5,9%). Conclusões: A esplenectomia subtotal com preservação do pólo inferior por videolaparoscopia em ratos foi exequível e segura. O polo inferior do baço mostrou-se viável em 94,1% dos animais. Descritores: Baço. Esplenectomia. Laparoscopia. Ratos.

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“…Paulo et al 22 observed the growth of the remaining tissue on the 80 th day. STPI performed in other models than the one described in our work, showed that the histological pattern of this pole becomes similar to normal splenic tissue in 45 dias 23 .…”

Section: Introductionmentioning

confidence: 98%

“…Paulo et al 22 Studies on immunohistochemistry and molecular genetics are being developed to complement this work in order to clarify these issues.…”

mentioning

confidence: 99%

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

Paulo

Nunes

et al. 2011

Acta Cir. Bras.

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PURPOSE: To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole. METHODS: Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) - treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days. The survival and weight of the animals were recorded. The inferior pole was measured, weighed and morphologically analyzed. RESULTS: All animals survived. The weight of the animals increased in all subgroups, but decreased on the 10th day in the subgroups treated with hyperbaric oxygen (p<0.001). The viability of the inferior pole was more evident in animals treated on the 15th day, but did not differ on the 45th day. The growth of the inferior pole has not occurred on the 15th day but on the 45th day after surgery in untreated animals (p<0.01) and treated animals (p<0.05). Vascular and cellular increase in treated animals was significantly higher than in untreated ones. CONCLUSION: Hyperbaric oxygen therapy did not affect the survival of animals but reduced their weight. It improved the viability of the inferior splenic pole, but did not interfere with their growth.

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Growth of the lower spleen pole remaining after subtotal splenectomy in rats

Cited by 5 publications

References 12 publications

Macro and microscopic comparison of the upper pole of the spleen with the lower pole in partial splenectomy

Macro and microscopic comparison of the upper pole of the spleen with the lower pole in partial splenectomy

Subtotal laparoscopic splenectomy in rats with preservation of the inferior pole

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

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