Growth on blood agar discriminates Mycobacterium avium and Mycobacterium intracellulare

Drancourt, Michel; Gevaudan, M. J.; Truffot, C; Jarlier, Vincent

doi:10.1046/j.1469-0691.2003.00706.x

Cited by 3 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent data have indicated that mycobacteria are in no way particular regarding their manipulation in routine clinical microbiology laboratories. Concerning isolation, routine sheep blood medium sustains the isolation and growth of the vast majority of mycobacteria encountered in clinical microbiology laboratories, including M. tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare (24,141,142), with the notable exception of M. leprae, which cannot be propagated outside animals (143) but can be cultured in the footpads of immunocompromised mice. In addition, sheep blood agar and sheep serum agar media have also been used to analyze in vitro susceptibility to antibiotics (144).…”

Section: Mycobacteriamentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

View full text Add to dashboard Cite

SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

show abstract

Section: Mycobacteriamentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

View full text Add to dashboard Cite

show abstract

Cell Extract-Containing Medium for Culture of Intracellular Fastidious Bacteria

et al. 2013

View full text Add to dashboard Cite

The culture of fastidious microorganisms is a critical step in infectious disease studies. As a proof-of-concept experiment, we evaluated an empirical medium containing eukaryotic cell extracts for its ability to support the growth of Coxiella burnetii. Here, we demonstrate the exponential growth of several bacterial strains, including the C. burnetii Nine Mile phase I and phase II strains, and C. burnetii isolates from humans and animals. Low-oxygen-tension conditions and the presence of small hydrophilic molecules and short peptides were critical for facilitating growth. Moreover, bacterial antigenicity was conserved, revealing the potential for this culture medium to be used in diagnostic tests and in the elaboration of vaccines against C. burnetii. We were also able to grow the majority of previously tested intracellular and fastidious bacterial species, including Tropheryma whipplei, Mycobacterium bovis, Leptospira spp., Borrelia spp., and most putative bioterrorism agents. However, we were unable to culture Rickettsia africae and Legionella spp. in this medium. The versatility of this medium should encourage its use as a replacement for the cell-based culture systems currently used for growing several facultative and putative intracellular bacterial species.

show abstract

Repertoire of Bacterial Species Cultured from the Human Oral Cavity and Respiratory Tract

et al. 2018

View full text Add to dashboard Cite

While the gut microbiota is currently in the spotlight, the airway microbiome has been recently associated with several pulmonary diseases and carcinogenesis. As there are several biases associated with high-throughput sequencing methods, cultivation techniques are crucial for the investigation of the human microbiome. We thus aimed to build an exhaustive database, including a list of microbes isolated by culture from respiratory specimens, by performing a review of the literature. Herein, we have listed a total of 756 species cultured from the human respiratory tract. This represents 27.23% of the overall bacterial richness captured from human being by culture methods. This repertoire could be valuable for the elucidation of the interactions between the respiratory microbiome and human health.

show abstract

Growth on blood agar discriminates Mycobacterium avium and Mycobacterium intracellulare

Cited by 3 publications

References 7 publications

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

Cell Extract-Containing Medium for Culture of Intracellular Fastidious Bacteria

Repertoire of Bacterial Species Cultured from the Human Oral Cavity and Respiratory Tract

Contact Info

Product

Resources

About