Growth pattern of tumor xenografts in wistar rats after treatment with cyclophosphamide, total lymphoid irradiation and/or cyclosporin A

Hoogenhout, J.; Kazem, I.; Jerusalem, C; Bakkeren, J. A. J. M.; Jong, Jan de; Kal, Henk B.; Munster, P. J. J. Van

doi:10.1016/0360-3016(83)90014-7

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…Hoogenhout and his colleagues used a cyclosporin treatment that was similar to ours (20-30mg/kg on alternate days), but transplanted human colon adenocarcinoma fragments regressed [29]. However, they used subcutaneous implantation, whereas we used transplantation under the renal capsule [7,32,33].…”

Section: Discussionmentioning

confidence: 97%

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Implanted Solid Human Tumours Grow Under the Renal Capsule of Cyclosporin‐Immunosuppressed Rats

et al. 1994

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Cyclosporin (CsA) is a potent immunosuppressive drug widely used in organ transplantation. We transplanted fresh surgical samples from human solid malignant tumours into 45 CsA-immunosuppressed rats. Eight out of nine tumour types grew and remained viable for 5 weeks or more in at least two of the transplanted rats. In 29 rats (64%) a distinct growth of primary human tumours was recorded. Five malignancies (intestinal-type gastric carcinoma, adenocarcinoma of the lung, lymph node metastasis of a testicular teratocarcinoma, soft tissue malignant fibrous histiocytoma (MFH), and small-cell sarcoma) showed invasive and progressive growth. In all five cases the largest tumours were 0.9 cm or over in diameter when the rats were killed 5-9 weeks after transplantation. In three cases (adenocarcinoma of the colon, hypernephroma, and a second MFH) the growth of the implants under the kidney capsule was slow, but small living tumour transplants were still found 3-6 weeks later. In every case the microscopic morphology of the xenograft tumour was identical with the original tumour. In two cases the primary xenografts (teratocarcinoma and small-cell sarcoma) were retransplanted into 11 CsA-immunosuppressed rats. In both types the second passage tumours grew, and the take-off and growth rates were comparable to the primary xenografts. Cyclosporin-treated laboratory rats are an alternative to immunodeficient nude and SCID mice for growing fresh human tumour transplants in vivo. Although a few infections were encountered, most of the rats survived the CsA treatment well for up to 2 months.

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Section: Discussionmentioning

confidence: 97%

“…We have also reported that several human in vitro cultured cell lines grow invasively in CsA-treated rats [23]. Hoogenbout and his colleagues attempted to grow tumour fragments of a human colon adenocarcinoma in five CsA-immunosuppressed rats but the xenograft tumours regressed [29].…”

Section: Discussionmentioning

confidence: 99%

Implanted Solid Human Tumours Grow Under the Renal Capsule of Cyclosporin‐Immunosuppressed Rats

et al. 1994

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“…Wistar rats (n = 65) were used to make the animal model of HCC. After anesthesia, each animal received an implantation of 4 × 10 6 tumor cells suspended in culture medium without serum and daily cyclosporin injections (10 μg/g) intraperitoneally [17] . When the tumors were grown, animals were used for the further experiments.…”

Section: Construction Of Hcc Animal Model In Ratmentioning

confidence: 99%

Selective tropism of liver stem cells to hepatocellular carcinomain vivo

Zhong

He²,

Wu³

et al. 2007

WJG

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“…Therefore, a temporally immunosuppressed animal model that will not reject human cancer cells at the starting period where afterwards a reversible suppressant can be achieved after withdrawal is crucial for understanding the phenomenon. Kaartinen et al [6] and Hoogenhout et al [4] showed that Wistar rats treated with cyclosporin A develop a state of immune suppression that permits the growth of tumor xenografts. It was also demonstrated that in these models there was no alteration in the tumor doubling time or histological morphology of the xenografts in the adapted host when compared to those in the donor tumors.…”

Section: Introductionmentioning

confidence: 99%

Visualization of Inflammation at Early Stage of Lung Cancer in Xenografted Temporally Immunosuppression Rats by Ferrioxamine Magnetic Resonance Imaging

Dechsupa

Udomtanakunchai

Udom-Utraracheva

et al. 2016

International Journal of Molecular Imaging

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Physiological responses such as chronic inflammation and angiogenesis could be used as biomarkers for early detection of cancer with noninvasive imaging modalities. The present study reports the application of magnetic resonance imaging instrument to image the binding of ferrioxamine with hemin that allows visualizing the chronic inflammation foci of lung tissue of immunocompromised rats xenografted using small cell lung carcinoma. A low concentration of ferrioxamine (0.05 ± 0.02 μM·kg−1 of rat weight) deposited on tissue outside the vasculature was found to diffuse across the capillary walls to the interstitial space and inflammation foci, which provided a clear enhancement of T1-weighted gradient-echo sequence images. Ferrioxamine imaging allowed the determination of inflammatory sites and their localization in 3D fat-suppressed maximum intensity projections. The smallest dimension of foci that can be clearly determined is about 0.1 mm3. In concomitant to the in vivo imaging, analysis of histological tissue section showed the development of inflammatory sites. This study provides evidence that medical imaging instrument such as MRI scanner allows researchers to correlate images taken with MRI with those using high-resolution microscopy. Moreover, ferrioxamine is a useful molecular probe for determining chronic inflammation particularly at the very early stages of cancer.

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Growth pattern of tumor xenografts in wistar rats after treatment with cyclophosphamide, total lymphoid irradiation and/or cyclosporin A

Cited by 8 publications

References 15 publications

Implanted Solid Human Tumours Grow Under the Renal Capsule of Cyclosporin‐Immunosuppressed Rats

Implanted Solid Human Tumours Grow Under the Renal Capsule of Cyclosporin‐Immunosuppressed Rats

Selective tropism of liver stem cells to hepatocellular carcinomain vivo

Visualization of Inflammation at Early Stage of Lung Cancer in Xenografted Temporally Immunosuppression Rats by Ferrioxamine Magnetic Resonance Imaging

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