Growth performance and digestion improvement of juvenile sea cucumber Apostichopus japonicus fed by solid-state fermentation diet

Abstract

In this study, the compound probiotics including Pseudoalteromonas. Sp D11, Bacillus subtilis A142, Saccharomyces cerevisiae Y23 and Lactobacillus plantarum L54 were applied for solid-state fermenting the artificial diet of juvenile sea cucumber Apostichopus japonicus. The quality of solid-state fermented (SSF) feed and its potential effects on growth performance and digestive enzyme activities were investigated. The results showed that, compared to unfermented diet, SSF feed had a better palatability quality; meanwhile, a higher ratio of crude protein, free amino acids and reducing sugars and a lower ratio of crude fibre, crude ash and alginic acid were found in SSF feed. The results of the feeding trial showed that feeding SSF feed could improve growth performance of sea cucumbers more than those fed with the unfermented diet. The results of digestive enzyme assays showed that feeding SSF feed to animals could significantly improve activities of amylase, cellulase, alginic acid enzyme and protease, which implied that fermented feed could improve digestibility of A. japonicus. In summary, the SSF feed fermented by compound probiotics had better palatability and nutrition value than unfermented feed. And feeding SSF feed could significantly improve the growth performance and digestion ability for sea cucumbers in the phases of nursery and grow-out. K E Y W O R D Sdigestive enzymes, growth performance, sea cucumber, solid-state fermented feed

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Intestinal microbiota analysis and probiotic application in gamat sea cucumber ( Stichopus horrens )

“…Thus, the fermentation process should be investigated to inform the optimization of the nutritional regime for cultured sea cucumber. This conclusion was supported by Wang et al (2017) who developed a feeding regime for sea cucumber ( A. japonicus ) using a solid‐state fermentation diet. Their results showed that palatability and nutritional values of fermented feed combined with probiotics were better than those of unfermented feed for sea cucumber in the nursery phase.…”

Effects of feed supplemented with probiotics on the culture of sea cucumber Apostichopus japonicus

“…In our study, probiotics are mixed with formulated feed and then incubated at 30°C for 8 h. The incubation process may increase the number of microorganisms in probiotics and stimulate the degradation of protein in formulated feed. Fermented feed could improve the growth performance of sea cucumber because the fermentation process could increase the content of small peptide and free amino acid in feed (Feng et al, 2020;Wang et al, 2017).…”