Growth performance and reproductive function impairment of glyphosate‐based herbicide in male guinea pig (<i>Cavia porcellus</i>)

Mutwedu, Valence Bwana; Nyongesa, Albert W.; Azine, Pascaline Ciza; Chiregereza, Daniel Katulanya; Ngoumtsop, Victor Herman; Mugumaarhahama, Yannick; Ayagirwe, Rodrigue Basengere Balthazar

doi:10.1002/vms3.443

Cited by 16 publications

(14 citation statements)

References 42 publications

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“…In our study, sperm motility was reduced at D5, D13 and D25 after RU exposure. These data are in good agreement with those observed in vivo in rats [39][40][41], mice [8], and pigs [42,43] and in vitro in humans [44,45]. In addition, the calcium and ATP concentrations in spermatozoa were significantly reduced in RU animals.…”

Section: Discussionsupporting

confidence: 90%

“…No impact on sperm concentration was recorded after dietary RU exposure, which contrasts with previous findings. Indeed, an exposure to G (500 mg/kg bw/d) for 5 weeks [50] or to RU (50 mg/kg bw/d) during the gestational period [9] or during adulthood in rats [40], mice [8], pigs [42] and in rabbits [51] led to a reduced sperm concentration. However, the effect of RU or G on the sperm concentration could be dependent on the species but also on the timing of exposure.…”

Section: Discussionmentioning

confidence: 99%

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Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

Serra

Estienne

Bourdon

et al. 2021

Toxics

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The effects of chronic dietary Roundup (RU) exposure on rooster sperm parameters, fertility, and offspring are unknown. We investigated the effects of chronic RU dietary exposure (46.8 mg kg−1 day−1 glyphosate) for 5 weeks in 32-week-old roosters (n = 5 RU-exposed and n = 5 control (CT)). Although the concentrations of glyphosate and its main metabolite AMPA (aminomethylphosphonic acid) increased in blood plasma and seminal fluid during exposure, no significant differences in testis weight and sperm concentrations were observed between RU and CT roosters. However, sperm motility was significantly reduced, associated with decreased calcium and ATP concentrations in RU spermatozoa. Plasma testosterone and oestradiol concentrations increased in RU roosters. These negative effects ceased 14 days after RU removal from the diet. Epigenetic analysis showed a global DNA hypomethylation in RU roosters. After artificial insemination of hens (n = 40) with sperm from CT or RU roosters, eggs were collected and artificially incubated. Embryo viability did not differ, but chicks from RU roosters (n = 118) had a higher food consumption, body weight and subcutaneous adipose tissue content. Chronic dietary RU exposure in roosters reduces sperm motility and increases plasma testosterone levels, growth performance, and fattening in offspring.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

Serra

Estienne

Bourdon

et al. 2021

Toxics

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“…both vertebrate and invertebrates [3][4][5], as well as in wildlife and aquatic species where this contaminant, alone or in formulation, induced oxidative stress an damage. These observations are raising concern about public health since GLY detected in the urine of humans [11].…”

Section: Metabolomic Characterization Of the Livermentioning

confidence: 99%

“…Limits for the presence of GLY in drinking water range from the highest levels in Australia (1000 µg/L) and USA (700 µg/L) to lower levels in Canada (280 µg/L) and Europe (0.1 µg/L) [ 2 ]. Despite the claim that GLY-based herbicides only target plant-specific enzymatic pathway, various studies demonstrated adverse effects of this contaminant in both vertebrate and invertebrates [ 3 , 4 , 5 ], as well as in wildlife and aquatic species [ 6 , 7 , 8 , 9 , 10 ], where this contaminant, alone or in formulation, induced oxidative stress and DNA damage. These observations are raising concern about public health since GLY can be detected in the urine of humans [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and Transcript Analysis Revealed a Sex-Specific Effect of Glyphosate in Zebrafish Liver

Giommi

Ladisa

Carnevali

et al. 2022

IJMS

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Glyphosate is a component of commonly used herbicides for controlling weeds in crops, gardens and municipal parks. There is increasing awareness that glyphosate-based herbicides, in addition to acting on plants, may also exert toxicity in wildlife and humans. In this study, male and female adult zebrafish were exposed to 700 µg/L of glyphosate (GLY), for 28 days. We used the metabolomic approach and UHPLC-ESI-MS to analyze liver samples to investigate the adverse effects of glyphosate on hepatic metabolism. The impact of GLY was found to be sex-specific. In female, GLY exposure affected purine metabolism by decreasing the levels of AMP, GMP and inosinic acid, consequently increasing uric acid levels with respect to the control (CTRL). Exposure to GLY also caused a decrease of UMP levels in the pyrimidine metabolism pathway. In male, GLY exposure decreased the aminoadipic acid within the lysine degradation pathway. Transcript analysis of genes involved in stress response, oxidative stress and the immune system were also performed. Results demonstrated an increased stress response in both sexes, as suggested by higher nr3c1 expression. However, the hsp70.2 transcript level was increased in female but decreased in male. The results demonstrated reduced sod1, sod2, and gpx1a in male following exposure to GLY, indicating an impaired oxidative stress response. At the same time, an increase in the cat transcript level in female was observed. mRNA levels of the pro-inflammatory interleukins litaf and cxcl8b.1 were increased in female. Taken together, the results provide evidence of disrupted nucleotide hepatic metabolism, increased stress inflammatory response in female and disruption of oxidative stress response in male.

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“…Oxidative stress is defined as a physiological disturbance when the production of potentially destructive reactive oxygen species (ROS) exceeds the body's own natural antioxidant defense (Tremellen, 2008;Nimse and Pal, 2015;Mohammed et al, 2020). It has been associated with several chronic diseases such as diabetes, hypertension, inflammation, cancer, reproductive impairment in both humans and livestock (Nimse and Pal, 2015;Sevindik et al, 2018;Mutwedu et al, 2021) through DNA damage, lipid peroxidation, tissue injury and protein degradation (Unuigbe et al, 2014;Pehlivan et al, 2018). Research findings have shown that humans affected with such ailments often revert to use of synthetic antioxidants, which have been proven to quench or trap ROS (Nimse and Pal, 2015;Sevindik, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profile and Antioxidant Activities of Aqueous Extract of Moringa oleifera (Lam) Collected from DR Congo and Kenya

Mutwedu

Nyongesa

Jafred

et al. 2022

Turkish JAF Sci.Tech.

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Moringa oleifera Lam. is one of the most used plants in traditional medicine because of its high antioxidant properties. The antioxidant value, nonetheless, depends on locality where the plant is grown as well as specific parts on the plant. In this study, a phytochemical and antioxidant activity comparison of M. oleifera leaves, seeds and barks were carried out. Fresh leaves, seeds and barks were collected from 2 to 3 years old M. oleifera trees of Bukavu city of South Kivu province in DRC and Masii village of Machackos County in Kenya. A total of 300g of each dried sample powder was mixed with 700 mL of distilled water. Qualitative and quantitative assessment of alkaloids, saponins, phenols, flavonoids, glycosides, terpenoids and tannins were performed following standard methods while the antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results indicate that only alkaloids were absent in leaves from Kenya and DRC while phenols, flavonoids and tannins were absent in barks. Glycoside in seeds from DRC had the highest concentration (6.17%) followed by alkaloids in seeds from Kenya (5.56%). There was low concentration of terpenoids and flavonoids in all samples compared to other compounds. The highest extract yield was found in leaves from DR Congo (22.5%) and seeds from Kenya (20%). At the highest concentration (10 µg/mL), leaves from Kenya (88.29±1.12 µg/mL) and DRC (80.17±3.59 µg/mL) had the highest percentage inhibition of reactive oxygen-free radicals but lower than the reference standard (92.63±2.76 µg/mL). Leaves from Kenya (23.59 μg/mL) and DRC (28.67 μg/mL) had the highest IC50 compared to mean values of seeds and barks from the two countries. M. oleifera leaves, especially from Kenya, are recommended as a satisfactory antioxidant but can be substituted with seeds and/or barks in order to alleviate the use of leaves which are overused these days.

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Growth performance and reproductive function impairment of glyphosate‐based herbicide in male guinea pig (Cavia porcellus)

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 16 publications

References 42 publications

Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

Metabolomic and Transcript Analysis Revealed a Sex-Specific Effect of Glyphosate in Zebrafish Liver

Phytochemical Profile and Antioxidant Activities of Aqueous Extract of Moringa oleifera (Lam) Collected from DR Congo and Kenya

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