Growth Performance and Transcriptomic Response of Warm-Acclimated Hybrid Abalone Haliotis rufescens (♀) × H. corrugata (♂)

Tripp-Valdez, Miguel A.; Cicala, Francesco; Galindo-Sánchez, Clara E.; Chacón-Ponce, K D; López-Landavery, Edgar A.; Dı́az, Fernando; Re-Araujo, Denisse; Cruz, Fabiola Lafarga-De la

doi:10.1007/s10126-020-10002-7

Cited by 15 publications

(7 citation statements)

References 58 publications

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“…This result is consistent with the result of ABT that SD has better temperature resistance. Some studies also have been conducted to obtain hybrid abalones with better environmental adaptability: H. rufescens ♀ × H. corrugate ♂ [31], H. gigantea ♀ × H. discus hannai ♂ [29], H. hannai ♀ × H. fulgens ♂ [29,32], H. rubra ♀ × H. laevigata ♂ [33]. However, none of the molecular mechanisms explain heterosis in abalone, which is compounded by complex allelic and genic interactions, and epigenetic regulation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals the molecular mechanisms of heterosis on thermal resistance in hybrid abalone

et al. 2021

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Background Heterosis has been exploited for decades in different animals and crops due to it resulting in dramatic increases in yield and adaptability. Hybridization is a classical breeding method that can effectively improve the genetic characteristics of organisms through heterosis. Abalone has become an increasingly economically important aquaculture resource with high commercial value. However, due to changing climate, abalone is now facing serious threats of high temperature in summer. Interspecific hybrid abalone (Haliotis gigantea ♀ × H. discus hannai ♂, SD) has been cultured at large scale in southern China and has been shown high survival rates under heat stress in summer. Therefore, SD has become a good model material for heterosis research, but the molecular basis of heterosis remains elusive. Results Heterosis in thermal tolerance of SD was verified through Arrhenius break temperatures (ABT) of cardiac performance in this study. Then RNA-Sequencing was conducted to obtain gene expression patterns and alternative splicing events at control temperature (20 °C) and heat stress temperature (30 °C). A total of 356 (317 genes), 476 (435genes), and 876 (726 genes) significantly diverged alternative splicing events were identified in H. discus hannai (DD), H. gigantea (SS), and SD in response to heat stress, respectively. In the heat stress groups, 93.37% (20,512 of 21,969) of the expressed genes showed non-additive expression patterns, and over-dominance expression patterns of genes account for the highest proportion (40.15%). KEGG pathway enrichment analysis showed that the overlapping genes among common DEGs and NAGs were significantly enriched in protein processing in the endoplasmic reticulum, mitophagy, and NF-κB signaling pathway. In addition, we found that among these overlap genes, 39 genes had undergone alternative splicing events in SD. These pathways and genes may play an important role in the thermal resistance of hybrid abalone. Conclusion More alternative splicing events and non-additive expressed genes were detected in hybrid under heat stress and this may contribute to its thermal heterosis. These results might provide clues as to how hybrid abalone has a better physiological regulation ability than its parents under heat stress, to increase our understanding of heterosis in abalone.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals the molecular mechanisms of heterosis on thermal resistance in hybrid abalone

et al. 2021

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“…The growth rate of interspecific hybrids between the black drum ( Pogonias cromis ) and red drum ( Sciaenops ocellatus ) was significantly higher than both parents [ 37 ]. Hybrid progenies of the red abalone (female Haliotis rufescens ) and the pink abalone (male H. corrugate ) have significantly elevated growth and survival during conditions of thermal stress (22 °C) [ 38 ]. Hybrid striped bass was found to be superior in survival rate than the two parent species, white bass ( Morone chrysops ) and striped bass ( Morone saxatilis ).…”

Section: Introductionmentioning

confidence: 99%

Environment-Dependent Heterosis and Transgressive Gene Expression in Reciprocal Hybrids between the Channel Catfish Ictalurus punctatus and the Blue Catfish Ictalurus furcatus

Wang

Bruce

et al. 2022

Biology

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The hybrid between female channel catfish (Ictalurus punctatus) and male blue catfish (Ictalurus furcatus) is superior in feed conversion, disease resistance, carcass yield, and harvestability compared to both parental species. However, heterosis and heterobeltiosis only occur in pond culture, and channel catfish grow much faster than the other genetic types in small culture units. This environment-dependent heterosis is intriguing, but the underlying genetic mechanisms are not well understood. In this study, phenotypic characterization and transcriptomic analyses were performed in the channel catfish, blue catfish, and their reciprocal F1s reared in tanks. The results showed that the channel catfish is superior in growth-related morphometrics, presumably due to significantly lower innate immune function, as investigated by reduced lysozyme activity and alternative complement activity. RNA-seq analysis revealed that genes involved in fatty acid metabolism/transport are significantly upregulated in channel catfish compared to blue catfish and hybrids, which also contributes to the growth phenotype. Interestingly, hybrids have a 40–80% elevation in blood glucose than the parental species, which can be explained by a phenomenon called transgressive expression (overexpression/underexpression in F1s than the parental species). A total of 1140 transgressive genes were identified in F1 hybrids, indicating that 8.5% of the transcriptome displayed transgressive expression. Transgressive genes upregulated in F1s are enriched for glycan degradation function, directly related to the increase in blood glucose level. This study is the first to explore molecular mechanisms of environment-dependent heterosis/heterobeltiosis in a vertebrate species and sheds light on the regulation and evolution of heterosis vs. hybrid incompatibility.

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“…The fact that many aquatic animals such as fishes and shellfishes produce offspring with improved traits through interspecific hybridization is widely exploited in aquaculture [ 12 , 36 , 37 , 38 ]. In crosses of O. niloticus × O. aureus , 100% of hybrid tilapia are male [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Integration of mRNA and miRNA Profiling Reveals Heterosis in Oreochromis niloticus × O. aureus Hybrid Tilapia

Xiao

Chen

Wang

et al. 2022

Animals

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Heterosis is a widespread biological phenomenon in fishes, in which hybrids have superior traits to parents. However, the underlying molecular basis for heterosis remains uncertain. Heterosis in growth and survival rates is apparent in hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂). Comparisons of growth and hematological biochemical characteristics and mRNA and miRNA transcriptional analyses were performed in hybrid and parents tilapia stocks to investigate the underlying molecular basis for heterosis. Growth characteristics and hematological glucose and cholesterol parameters were significantly improved in hybrids. Of 3097 differentially expressed genes (DEGs) and 120 differentially expressed miRNAs (DEMs) identified among three stocks (O. niloticus, O. aureus, and hybrids), 1598 DEGs and 62 DEMs were non-additively expressed in hybrids. Both expression level dominance and overdominance patterns occurred for DEGs and DEMs, indicating that dominance and overdominance models are widespread in the transcriptional and post-transcriptional regulation of genes involved in growth, metabolism, immunity, and antioxidant capacity in hybrid tilapia. Moreover, potential negative regulation networks between DEMs and predicted target DEGs revealed that most DEGs from miRNA-mRNA pairs are up-regulated. Dominance and overdominance models in levels of transcriptome and miRNAome facilitate the integration of advantageous parental alleles into hybrids, contributing to heterosis of growth and improved survival. The present study provides new insights into molecular heterosis in hybrid tilapia, advancing our understanding of the complex mechanisms involved in this phenomenon in aquatic animals.

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Growth Performance and Transcriptomic Response of Warm-Acclimated Hybrid Abalone Haliotis rufescens (♀) × H. corrugata (♂)

Cited by 15 publications

References 58 publications

Transcriptome analysis reveals the molecular mechanisms of heterosis on thermal resistance in hybrid abalone

Transcriptome analysis reveals the molecular mechanisms of heterosis on thermal resistance in hybrid abalone

Environment-Dependent Heterosis and Transgressive Gene Expression in Reciprocal Hybrids between the Channel Catfish Ictalurus punctatus and the Blue Catfish Ictalurus furcatus

Integration of mRNA and miRNA Profiling Reveals Heterosis in Oreochromis niloticus × O. aureus Hybrid Tilapia

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