Growth performance, blood variables, intestinal bacterial content, and morphological measurements of broilers supplemented with Lactobacillus casei-fermented mixture of red rice and aromatic ginger

Abstract

Background and Aim: Natural feed additives are important for broiler sustainability during the post-antibiotic era. This study aimed to evaluate the possible effects of the dietary supplementation of Lactobacillus casei-fermented mixture of red rice and aromatic ginger (FERMIX) on growth, blood profiles, intestinal bacterial content, and morphological measurements of broilers. Materials and Methods: Two hundred broiler chicks were allotted to four groups with five replications, including CONTROL (chicks provided with basal feed), FERMIX025 (basal feed supplemented with 0.25% FERMIX), FERMIX050 (0.50% FERMIX), and FERMIX100 (1.00% FERMIX). FERMIX is an anaerobic fermentation product from a mixture of red rice flour and aromatic ginger using L. casei. Blood, organs, digesta, and carcass were collected on day 35. Results: Final body weight, weight gain, feed intake, and feed conversion ratio did not differ (p>0.05) across treatments. FERMIX at 0.5% increased (p<0.05) spleen relative weight. Liver weight was lower (p<0.05) in broilers fed with 1% FERMIX. The liver weight linearly reduced (p<0.05) with the increased FERMIX levels. FERMIX at 0.25% elevated (p<0.05) broiler drumsticks' yield than that in other groups. Erythrocytes, hemoglobin, packed cell volume, and plasma total protein levels were quadratically higher (p<0.05) in FERMIX050 treated than in other groups. Increased FERMIX levels resulted in a linear decrease (p=0.08) in ileal lactose-negative Enterobacteriaceae counts. Villi height/crypt depth (CD) ratio was quadratically higher (p<0.05) in the duodenum of broiler supplemented with 0.5% FERMIX. The jejunal CD was quadratically lower (p<0.05) in FERMIX050 than in other groups. Conclusion: Although it did not influence growth, dietary FERMIX, especially at 0.5%, improved immune competencies, physiological conditions, and health of broilers' intestine.

Effect of Dietary Fenugreek Seeds and Synbiotic on the Physiological Anatomical, Immunological Attributes and Antioxidant Status in Stressed Chickens

“…The same outcome was stated by Elamin et al [55], that were no significant differences in relative and absolute weights of liver, gizzard and intestine of broiler chickens Ross 308 at 6 weeks fed diet containing 0.50 and 0.75% FSP. Also, this observation corroborated the data of Astuti et al [47], who found that 0.25, 0.5 and 1% dietary synbiotic (FERMIX) comprising mixture of red rice and aromatic ginger as prebiotic fermented anaerobically using Lactobacillus casei (probiotic) had no effect on relative weights of heart, pancreas, gizzard, small intestine segments and ceaca although high liver relative weight was achieved by 0.5% FERMIX at 35 days of age in broilers.…”

“…High PCV in T3 and T5 could be related to positive infulence of synbiotic composition for stimulation of morphological changes in intestine and increase the absorption capacity and feed efficiency which may in turn induce the increase of red blood cells and PCV for accelerate metabolism under stress [18]. This was confirmed recently by Astuti et al [47], that 0.25 and 0.50% of synbiotic led to enhance synthesis of erythrocytes which reflect on increased hemoglobin and PCV of broilers. Low H/L could be explained by effect of synbiotic to relieve stress and improve resistance against NC and IBD (table 4) and this was similar to what obtained by Abdel Razek and Tony [48].…”

Survivability of Lactobacillus plantarum in nutmeg (Myristica fragrans Houtt) flesh extract and its effect on the performance of broiler chicken

“…According to Sarangi et al [ 42 ], adding mannanoligosaccharide and the synbiotics L. bulgaricus, L. plantarum , Streptococcus faecium, Bifidobacterium bifidus, and Saccharomyces cerevisiae to the ration had no impact on the proportion of internal organs and broiler carcasses. In agreement with this, Astuti et al [ 43 ] discovered that feeding broiler chicks a mixture of fermented brown rice and ginger with Lactobacillus casei had no impact on the carcasses or the relative weight of internal organs. This reveals that the synbiotic administration of nutmeg flesh extract and L. plantarum has no detrimental impact on the features of broiler chicken carcasses.…”

The Effects of Lactobacillus farciminis and Lactobacillus rhamnosus on Growth, Blood Biochemical, and Meat Quality Indicators of Specific Pathogen‐Free Broiler Chickens

“…According to our research methodology, and based on similar previous studies [ 10 , 11 ], blood samples were collected from the wing vein of 45 randomly selected broilers of each group at the end of the study. The samples were examined in the laboratory of the Veterinary Clinic of Latvia University of Life Sciences and Technologies, using a Mindray BS200 Clinical Analyzer, with the absorbance photometry method.…”

“…In several studies, broilers fed probiotics containing various Lactobacillus spp. presented significantly higher amounts of total protein, including albumin and globulin, as well as significantly lower concentrations of cholesterol, total lipids, and triglycerides in the blood [ 5 , 9 , 10 ]. However, in several studies, when broilers were fed probiotics that included Lactobacillus spp., there were no significant changes in the biochemical blood parameters at the end of the study [ 7 , 8 , 11 ].…”