Growth pH and transient increases in amino acid availability influence polyglucose synthesis by Fusobacterium nucleatum grown in continuous culture

Zilm, Peter

doi:10.1016/s0378-1097(02)00965-5

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Studies have shown that at lower pH values, nicotine is less toxic. However, the increase in the concentration of nicotine in the medium may cause alkalinization and higher absorption of nicotine by the cells, and thus lead to an increase in the production of biofilm and its toxicity 50) .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial behavior of titanium coating with chlorhexidine-doped thin film exposed to a biofilm supplemented with nicotine

et al. 2023

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Because nicotine upregulates the growth of most oral bacteria, this in vitro study investigated the antimicrobial effect of chlorhexidinedoped thin film on commercially pure titanium against Fusobacterium nucleatum (F. nucleatum) biofilm supplemented with different concentrations of nicotine (0, 1, and 2 mg/mL). Biofilms were formed on a chlorhexidine-doped thin film on commercially-puretitanium discs and compared to the control groups. Biofilm viability, total biofilm growth using a spectrophotometer, extracellular polysaccharide content, and pH variations were assessed as dependent variables. Data were submitted to ANOVA and Tukey honest significant difference tests (α=0.05). F. nucleatum biofilm growth was inhibited when exposed to chlorhexidine-doped thin film (p<0.05). Biofilm supplemented with nicotine did not impact the synthesis of EPS on the same type of treatment (p>0.05). The pH values were significantly increased with the increase of nicotine concentration (p<0.05). Chlorhexidine-doped thin film was effective in reducing F. nucleatum biofilm supplemented with nicotine.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial behavior of titanium coating with chlorhexidine-doped thin film exposed to a biofilm supplemented with nicotine

et al. 2023

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“…We interpret this to mean that S. aureus adapts to SCFAs by modifying its physiology rather than directly detoxifying them via degradation. The increased acetate levels are likely due to S. aureus utilization of glucose remaining in Fn CFS, as F. nucleatum preferentially ferments amino acids 20,21 . Given that S. aureus growth is similarly impaired when BB is supplemented with the sodium salts of acetate (5mM), propionate (5mM), and butyrate (15mM)(Fig.…”

Section: S Aureus Growth Is Impaired By F Nucleatummentioning

confidence: 99%

Staphylococcus aureus overcomes anaerobe-derived short-chain fatty acid stress via FadX and the CodY regulon

Fletcher

Villareal

Penningroth

et al. 2022

Preprint

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Chronic rhinosinusitis (CRS) is characterized by immune dysfunction, mucus hypersecretion, and persistent infection of the paranasal sinuses. While Staphylococcus aureus is a primary CRS pathogen, recent sequence-based surveys have found increased relative abundances of anaerobic bacteria, suggesting that S. aureus may experience altered metabolic landscapes in CRS relative to healthy airways. To test this possibility, we characterized the growth kinetics and transcriptome of S. aureus in supernatants of the abundant CRS anaerobe Fusobacterium nucleatum. While growth was initially delayed, S. aureus ultimately grew to similar levels as in the control medium. The transcriptome was significantly affected by F. nucleatum metabolites, with the agr quorum sensing system notably repressed. Conversely, expression of fadX, encoding a putative propionate coA-transferase, was significantly increased, leading to our hypothesis that short chain fatty acids (SCFAs) produced by F. nucleatum could mediate S. aureus growth behavior and gene expression. Supplementation with propionate and butyrate, but not acetate, recapitulated delayed growth phenotypes observed in F. nucleatum supernatants. A fadX mutant was found to be more sensitive than wild type to propionate, suggesting a role for FadX in the S. aureus SCFA stress response. Interestingly, spontaneous resistance to butyrate, but not propionate, was frequently observed. Whole genome sequencing and targeted mutagenesis identified codY mutants as resistant to butyrate inhibition. Together, these data show that S. aureus physiology is dependent on its co-colonizing microbiota and metabolites they exchange, and indicate that propionate and butyrate may act on different targets in S. aureus to suppress its growth.

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“…The continued fermentation of glutamate inhibits IP breakdown in anaerobic cultures but, as glutamate becomes depleted, IP is catabolised to a mixture of butyric, acetic, lactic and formic acids (11). In contrast to resting cells, studies on F. nucleatum growing in continuous culture at pH 7.4 (18) have shown that, at steady state, little IP is stored intracellularly when the cells are fermenting both glucose and energy‐yielding amino acids. Under these conditions, IP is metabolised and the energy derived is used to maximise cell numbers.…”

Section: The Effect Of Fructose and Energy Yielding Amino Acids On Thmentioning

confidence: 99%

Changes in growth and polyglucose synthesis in response to fructose metabolism byFusobacterium nucleatumgrown in continuous culture

Zilm

Gully

Rogers

2003

Oral Microbiology and Immunology

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Fusobacterium nucleatum, grown in a chemically defined medium at micro(rel) = 0.5, produced greater cell yields and undetectable levels of intracellular polyglucose (IP) when fructose was substituted for glucose. The utilisation and metabolism of fructose by growing cells was studied and the effect of the energy-yielding amino acids, glutamate, serine, histidine and lysine on cell yield, IP synthesis and acidic end-products was investigated. When F. nucleatum was grown on elevated amino acid levels, IP was synthesised from fructose and amino acids were metabolised to lactate, acetate, butyrate and formate. Under these conditions, IP synthesis was associated with the cells being replete with amino acid-derived energy; an observation supported by the absence of IP when the levels of (energy yielding) amino acids were reduced. Compared with fructose, glucose was less efficiently removed from the growth medium and produced less biomass and markedly lower levels of IP during energy-limited growth.

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Growth pH and transient increases in amino acid availability influence polyglucose synthesis by Fusobacterium nucleatum grown in continuous culture

Cited by 3 publications

References 15 publications

Antimicrobial behavior of titanium coating with chlorhexidine-doped thin film exposed to a biofilm supplemented with nicotine

Antimicrobial behavior of titanium coating with chlorhexidine-doped thin film exposed to a biofilm supplemented with nicotine

Staphylococcus aureus overcomes anaerobe-derived short-chain fatty acid stress via FadX and the CodY regulon

Changes in growth and polyglucose synthesis in response to fructose metabolism byFusobacterium nucleatumgrown in continuous culture

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