2003
DOI: 10.1128/iai.71.11.6510-6525.2003
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Growth Phase-Dependent Response ofHelicobacter pylorito Iron Starvation

Abstract: Iron is an essential nutrient that is often found in extremely limited available quantities within eukaryotic hosts. Because of this, many pathogenic bacteria have developed regulated networks of genes important for iron uptake and storage. In addition, it has been shown that many bacteria use available iron concentrations as a signal to regulate virulence gene expression. We have utilized DNA microarray technology to identify genes of the human pathogen Helicobacter pylori that are differentially regulated on… Show more

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Cited by 141 publications
(166 citation statements)
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“…Similar studies were also recently conducted in the closely related bacterium Helicobacter pylori to identify genes that were differentially regulated upon a growth-inhibiting shift to iron-starvation conditions (Merrell et al, 2003). Many known iron-regulated genes were identified as well as a number of genes whose regulation by iron concentration was not previously predicted, including the virulence genes cagA, vacA and napA.…”
Section: Discussionmentioning
confidence: 87%
See 1 more Smart Citation
“…Similar studies were also recently conducted in the closely related bacterium Helicobacter pylori to identify genes that were differentially regulated upon a growth-inhibiting shift to iron-starvation conditions (Merrell et al, 2003). Many known iron-regulated genes were identified as well as a number of genes whose regulation by iron concentration was not previously predicted, including the virulence genes cagA, vacA and napA.…”
Section: Discussionmentioning
confidence: 87%
“…Many known iron-regulated genes were identified as well as a number of genes whose regulation by iron concentration was not previously predicted, including the virulence genes cagA, vacA and napA. A bioinformatic analysis of the predicted promoter regions of the differentially regulated genes led to identification of several putative Fur boxes, suggesting a direct role for Fur in iron-dependent regulation of these genes (Merrell et al, 2003).…”
Section: Discussionmentioning
confidence: 99%
“…5A), thus confirming the identity of the samples, the proper expression of the transgenes, and the strong Fur basal activity under our experimental conditions. 75 of the 196 selected genes have already been identified in previous transcriptome studies as Fur-and/or iron-regulated genes in E. coli (17,42), Helicobacter pylori (38,(43)(44)(45), Neisseria meningitidis (46,47), Pseudomonas aeruginosa (48,49), Campylobacter jejuni (50), Shewanella oneidensis (51,52), and Pasteurella multicoda (53). Conversely we unveiled 121 genes that have not been reported by previous studies.…”
Section: Resultsmentioning
confidence: 99%
“…DNA array technology has proved to be a powerful technique for the study of global gene regulation in many organisms (Conway & Schoolnik, 2003), and has also been successfully applied to study alterations in H. pylori gene expression (Bury-Mone et al, 2004;Forsyth et al, 2002;Kim et al, 2004;Merrell et al, 2003aMerrell et al, , 2003bThompson et al, 2003;Wen et al, 2003) and genetic variation between isolates (Israel et al, 2001a;Salama et al, 2000). In this study, we have applied DNA array technology to growth experiments with the well-characterized H. pylori reference strain 26695, both to define the H. pylori responses to variation in iron availability and to identify new members of the H. pylori Fur regulon.…”
Section: Introductionmentioning
confidence: 99%