Growth-Phase-Related Changes in Reactive Oxygen Species Generation as a Cold Stress Response in Antarctic<i>Penicillium</i>Strains

Miteva‐Staleva, Jeny; Stefanova, Tsvetanka; Krumova, Ekaterina; Angelova, Maria

doi:10.5504/bbeq.2011.0131

Cited by 7 publications

(8 citation statements)

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“…Our previous results demonstrated that growth at low temperatures induced the generation of reactive oxygen species (ROS) in cells and their mitochondrial fractions in Antarctic fungi in a dose and agedependent manner. At the same time, cold-stress response was not dependent on the cold-adaptation of the model strains (Miteva-Staleva et al 2011).…”

Section: Introductionmentioning

confidence: 75%

“…Fungal survival mechanisms include production of bioactive compounds, coldactive enzymes and antifreeze proteins (Robinson 2001;Krishnan et al 2011;Godinho et al 2013) The link between low-temperature exposure and the manifestations of oxidative stress has attracted more scientific attention in recent years, as well as the involvement of antioxidant enzymes in survival under such drastic conditions. It should be noted that while the cellular response against cold stress has been studied in various bacteria and plants, little is known about the adaptation of fungi to survive at temperatures close to negative (Miteva-Staleva et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Polish Polar Research

Krumova,

Koeva,

Stoitsova

et al. 2021

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During the evolution organisms are subjected to the continuous impact of environmental factors. In recent years an increasing number of studies have focused on the physicochemical limits of life on Earth such as temperature, pressure, drought, salt content, pH, heavy metals, etc. Extreme environmental conditions disrupt the most important interactions that support the function and structure of biomolecules. For this reason, organisms inhabiting extreme habitats have recently become of particularly great interest. Although filamentous fungi are an important part of the polar ecosystem, information about their distribution and diversity, as well as their adaptation mechanisms, is insufficient. In the present study, the fungal strain Penicillium griseofulvum isolated from an Antarctic soil sample was used as a study model. The fungal cellular response against short term exposure to low temperature was observed. Our results clearly showed that short-term low temperature exposure caused oxidative stress in fungal cells and resulted in enhanced level of oxidative damaged proteins, accumulation of reserve carbohydrates and increased activity of the antioxidant enzyme defence. Ultrastructural changes in cell morphology were analysed. Different pattern of cell pathology provoked by the application of two stress temperatures was detected. Overall, this study aimed to observe the survival strategy of filamentous fungi in extremely cold habitats, and to acquire new knowledge about the relationship between low temperature and oxidative stress.

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Polish Polar Research

Krumova,

Koeva,

Stoitsova

et al. 2021

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“…A large amount of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), superoxide anion (O 2 •- ), and hydroxyl radical (OH•), are produced in cells when the strains encounter extreme environmental conditions, such as drought ( Noctor et al, 2014 ), high and low temperatures ( Miteva-Staleva et al, 2014 ; Nantapong et al, 2019 ), high salinity ( Song et al, 2018 ), low pH ( Guo et al, 2016 ), and high loads of heavy metals ( Neira et al, 2021 ). The massive accumulation of ROS in cells can kill the bacteria.…”

Section: Resultsmentioning

confidence: 99%

A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains

et al. 2023

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Acinetobacter is ubiquitous, and it has a high species diversity and a complex evolutionary pattern. To elucidate the mechanism of its high ability to adapt to various environment, 312 genomes of Acinetobacter strains were analyzed using the phylogenomic and comparative genomics methods. It was revealed that the Acinetobacter genus has an open pan-genome and strong genome plasticity. The pan-genome consists of 47,500 genes, with 818 shared by all the genomes of Acinetobacter, while 22,291 are unique genes. Although Acinetobacter strains do not have a complete glycolytic pathway to directly utilize glucose as carbon source, most of them harbored the n-alkane-degrading genes alkB/alkM (97.1% of tested strains) and almA (96.7% of tested strains), which were responsible for medium-and long-chain n-alkane terminal oxidation reaction, respectively. Most Acinetobacter strains also have catA (93.3% of tested strains) and benAB (92.0% of tested strains) genes that can degrade the aromatic compounds catechol and benzoic acid, respectively. These abilities enable the Acinetobacter strains to easily obtain carbon and energy sources from their environment for survival. The Acinetobacter strains can manage osmotic pressure by accumulating potassium and compatible solutes, including betaine, mannitol, trehalose, glutamic acid, and proline. They respond to oxidative stress by synthesizing superoxide dismutase, catalase, disulfide isomerase, and methionine sulfoxide reductase that repair the damage caused by reactive oxygen species. In addition, most Acinetobacter strains contain many efflux pump genes and resistance genes to manage antibiotic stress and can synthesize a variety of secondary metabolites, including arylpolyene, β-lactone and siderophores among others, to adapt to their environment. These genes enable Acinetobacter strains to survive extreme stresses. The genome of each Acinetobacter strain contained different numbers of prophages (0–12) and genomic islands (GIs) (6–70), and genes related to antibiotic resistance were found in the GIs. The phylogenetic analysis revealed that the alkM and almA genes have a similar evolutionary position with the core genome, indicating that they may have been acquired by vertical gene transfer from their ancestor, while catA, benA, benB and the antibiotic resistance genes could have been acquired by horizontal gene transfer from the other organisms.

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“…Therefore, the scavenging of ROS is an important mechanism for organisms to reduce oxidative damage and abiotic stress [ 49 ]. Ectomycorrhizal roots were reported to increase the content of active oxygen-scavenging enzymes in mycelium and host plant to alleviate temperature damage [ 50 ]. In this study, the SOD, POD and CAT activity of the three sensitive isolates and two tolerant C. geophilum isolates were both elevated under 30℃ treatment, and the SOD content of tolerant isolates was significantly higher than that of sensitive isolates.…”

Section: Discussionmentioning

confidence: 99%

Effects of High Temperature-Triggered Transcriptomics on the Physiological Adaptability of Cenococcum geophilum, an Ectomycorrhizal Fungus

et al. 2022

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High temperature stress caused by global warming presents a challenge to the healthy development of forestry. Cenococcum geophilum is a common ectomycorrhizal fungus (ECMF) in the forest system and has become an important fungus resource with application potential in forest vegetation restoration. In this study, three sensitive isolates of C. geophilum (ChCg01, JaCg144 and JaCg202) and three tolerant isolates of C. geophilum (ACg07, ChCg28 and ChCg100) were used to analyze the physiological and molecular responses to high temperature. The results showed that high temperature had a significant negative effect on the growth of sensitive isolates while promoting the growth of tolerant isolates. The antioxidative enzymes activity of C. geophilum isolates increased under high temperature stress, and the SOD activity of tolerant isolates (A07Cg and ChCg100) was higher than that of sensitive isolates (ChCg01 and JaCg202) significantly. The tolerant isolates secreted more succinate, while the sensitive isolates secreted more oxalic acid under high temperature stress. Comparative transcriptomic analysis showed that differentially expressed genes (DEGs) of six C. geophilum isolates were significantly enriched in “antioxidant” GO entry in the molecular. In addition, the “ABC transporters” pathway and the “glyoxylate and dicarboxylic acid metabolic” were shared in the three tolerant isolates and the three sensitive isolates, respectively. These results were further verified by RT-qPCR analysis. In conclusion, our findings suggest that C. geophilum can affect the organic acid secretion and increase antioxidant enzyme activity in response to high temperature by upregulating related genes.

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Growth-Phase-Related Changes in Reactive Oxygen Species Generation as a Cold Stress Response in AntarcticPenicilliumStrains

Cited by 7 publications

References 45 publications

Polish Polar Research

Polish Polar Research

A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains

Effects of High Temperature-Triggered Transcriptomics on the Physiological Adaptability of Cenococcum geophilum, an Ectomycorrhizal Fungus

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