Growth plate cartilage transplanted to the articular surface remodels into articular-like cartilage in a process promoted by the synovial joint microenvironment

Dou, Zelong; Chau, Michael; Baroncelli, Marta; Bendre, Ameya; Landman, Ellie; Ottosson, Lars; Nilsson, Ola

doi:10.1016/j.bonr.2020.100410

Cited by 2 publications

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“…However, a more likely explanation would be that the perichondrium has an inherent chondrogenic program that is modified by the local microenvironment of the joint. Specifically, that the joint microenvironment may inhibit hypertrophic differentiation and promote differentiation towards the superficial layer chondrocyte phenotype (53,54) . This hypothesis would explain our observation that the newly formed chondrocytes of the perichondrium underwent hypertrophic differentiation only some distance away from the surface, but never close to the surface where chondrocytes instead remained small and eventually attained a superficial chondrocyte-like phenotype.…”

Section: Discussionmentioning

confidence: 99%

Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

Dou

Muder

Baroncelli

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

Dou

Muder

Baroncelli

et al. 2021

Bone

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Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

Dou

Muder

Baroncelli

et al. 2020

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Reconstruction of articular surfaces destroyed by infection or trauma is hampered by the lack of suitable graft tissues. Perichondrium autotransplants have been used for this purpose. However, the role of the transplanted perichondrium in the healing of resurfaced joints have not been investigated. Perichondrial and periosteal tissues were harvested from rats hemizygous for a ubiquitously expressed enhanced green fluorescent protein (EGFP) transgene and transplanted into full-thickness articular cartilage defects at the trochlear groove of distal femur in wild-type littermates. As an additional control, cartilage defects were left without a transplant (no transplant control). Distal femurs were collected 3, 14, 56, 112 days after surgery. Transplanted cells and their progenies were readily detected in the defects of perichondrium and periosteum transplanted animals but not in defects left without a transplant. Perichondrium transplants expressed SOX9 and with time differentiated into a hyaline cartilage that expanded and filled out the defects with Col2a1-positive chondrocytes and a matrix rich in proteoglycans. Interestingly, at later timepoints the cartilaginous perichondrium transplants were actively remodeled into bone at the transplant-bone interface and at post-surgery day 112 EGFP-positive perichondrium cells at the articular surface were positive for Prg4. In addition, both perichondrium and periosteum transplants contributed cells to the subchondral bone and bone marrow, suggesting differentiation into osteoblast/osteocytes as well as bone marrow cells. In summary, we found that perichondrium transplanted to articular cartilage defects develops into an articular-like, hyaline cartilage that integrates with the subchondral bone, and is maintained for an extended time. The findings indicate that perichondrium is a suitable tissue for repair and engineering of articular cartilage.

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Growth plate cartilage transplanted to the articular surface remodels into articular-like cartilage in a process promoted by the synovial joint microenvironment

Cited by 2 publications

References 0 publications

Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

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