Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth

4.1 Control of Biotheraputics Glycosylation

“…Both, hydrocortisone and predinisolone, were shown to improve sialylation [73,74]. Higher concentrations of these glucocorticoids compared to DEX were required to increase sialic acid content to the same extent [73].…”

“…Glutamate [52] Replacing glutamine with glutamate improves galactosylation and complement-dependent cytotoxicity Glutamine [74] Adaptation of a cell line to glutamine-free growth improves the antennarity of N-linked glycoforms Glycine [76] Glycine supplementation improves glycosylation by increasing sialic acid content of glycoforms Proline [75] Proline supplementation increases sialylation in hyperosmotic cell culture Proline [76] Proline supplementation improves glycosylation by increasing sialic acid content of glycoforms Threonine [76] Threonine supplementation improves glycosylation by increasing sialic acid content of glycoforms…”

Exploring Packaged Microvesicle Proteome Composition of Chinese Hamster Ovary Secretome

“…Hence, the presence of cell-proliferation regulation-associated cell signaling and structural proteins could be linked with the preparation of cells for higher rate of proliferation in the upcoming log-phase. Cells in lag phase have been reported to high intercellular stress level compared to cells in log, stationary and death-phase [35]. The stress proteins present in the microvesicles could be expected to be associated with regulation of intracellular stress in lag-phase of culture.…”

Using glyco-engineering to produce therapeutic proteins

“…Mucin-type O-glycosylation in mammalian cells typically varies in the frequency of site occupancy and display a mixture of different structures [82]. In human cells, the generation of homogeneous mucin-type O-glycans is hampered by the large repertoire of competing glycosyltransferases ( Figure 5A and B).…”