Growth Profile and Hydrocarbonoclastic Potential of Microorganisms Isolated from Tarballs in the Bight of Bonny, Nigeria

Itah, Alfred Young; Essien, Joseph P.

doi:10.1007/s11274-004-6694-z

Cited by 71 publications

(45 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolates were screened for their ability to utilize hydrocarbon using the vapour phase transfer methods of Itah and Essien (2005) with slight modifications. Molten mineral salt agar (20ml volume) that has cooled to about 45°C was aseptically added to petri dishes containing aliquot and swirled gently to mix then allowed to set.…”

Section: Screen Test For Hydrocarbon Utilizing Bacteriamentioning

confidence: 99%

Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

Ndubuisi-Nnaji¹,

John²,

Ofon³

2016

Journal of Applied Sciences and Environmental Management

View full text Add to dashboard Cite

Section: Screen Test For Hydrocarbon Utilizing Bacteriamentioning

confidence: 99%

Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

Ndubuisi-Nnaji¹,

John²,

Ofon³

2016

Journal of Applied Sciences and Environmental Management

View full text Add to dashboard Cite

“…These findings are not unexpected, because there are numerous reports to show genetic diversity among degradative genes. Among 20 PAH-degrading isolates, strong hybridization signals with ndoB were only observed in seven of eight naphthalene-degrading Pseudomonas strains [8]. In 1989, Furukawa found that only five Pseudomonas species of 15 biphenyl-degrading Pseudomonas, Achromobacter, Alcaligenes, Moraxella and Arthrobacter strains showed significant hybridization to bphABC of Pseudomonas pseudoalcaligenes KF 707.…”

Section: Resultsmentioning

confidence: 99%

“…Supernatant was discarded and pellets allowed to dry then add 40µl of TE buffer. The plasmids were characterized using agarose gel electrophoresis [8]. The molecular weights of plasmids were visualized using UV transilluminator.…”

Section: Plasmid Extractionmentioning

confidence: 99%

Isolation and Identification of Plasmids of Bacteria from Petroleum Products Contaminated Soil

Udochukwu

2017

JBMOA

View full text Add to dashboard Cite

Isolation and Identification of Plasmids of Bacteria from Petroleum Products Contaminated Soil was carried out. Hydrocarbon utilizing bacteria were isolated from petroleum-products contaminated soil from the tank farm of NNPC/PPMC Depot in Benin City, Nigeria. Plasmid analysis of bacteria was carried out showing the presence of plasmids in seven isolates: Bacillus subtilis, Bacillus species, Arthorbacter species, Pseudomonas aeroginosa, Klebsiella aerogenes, Alcaligenes species, and Pseudomonas species. All the plasmids corresponds to 23kbp of the maker. The result of the DNA extracted and amplified in a PCR using the ndoB primers indicated no visible bands, showing that ndoB primers is not specific for bacteria isolates in the samples, hence there was no amplification. The none specification of ndoB gene of isolates does not imply the absence of plasmids DNA that confers utilizing ability to it, but that the utilizing gene could possibly be borne on the chromosomal DNA which enabled its persistence in the contaminated soil or other genes which were tested such as catechol 2,3 dioxygenase (C23DO) and monoxygenase could be present.

show abstract

“…Specific colonies were then sub-cultured on freshly prepared basal media and the pure cultures were characterized according to recommended procedures (Sneath and Holt, 1986;Alsina and Blanch, 1994;Barnett and Hunter, 1987). The ability of the microbial isolates to utilize the ore as their sole source of energy for growth was determined by the method of Okpokwasili and Okorie (1988) and Itah and Essien (2005) using the mineral salt medium (MSM) of Zajic and Supplison (1972). Briefly, 10 ml of MSM were dispensed into each test tube and then 2% (0.2 g) of the milled ore sample was added.…”

Section: Isolation and Determination Of Solubilizing Potentials Of MImentioning

confidence: 99%

Phosphorus removal from Nigerias Agbaja iron ore by solubilization with Eurotium herbariorum

Obot¹

2012

J. Microbiol. Antimicrob.

View full text Add to dashboard Cite

Phosphorus is an unwanted component of iron ore. Its removal from Nigeria's Agbaja iron ore by solubilization with Eurotium herbariorum isolated from the ore samples has been achieved using the submerged culture technique. Result obtained shows 61.48% removal of P in 10 weeks through the biomass growth and activities of the filamentous fungus. The biomass dry weight of the fungus increased from an initial 1 g (dry weight of inoculums) to a maximum 3.021 g weight in 3 weeks only to drop to 0.486 g by the end of experiment. The ability of the fungus to metabolize the phosphorus in ore was noticed to increase with biomass weight but hindered over time. Extended duration of degradation after 7th week did not necessarily bring about more P removal. Phosphorus utilization by the fungus resulted in its growth and concomitant production of acidic metabolic products and adsorption of detectable concentrations of iron, copper, lead, zinc, cadmium and manganese from the ore samples. It is therefore suggestive that the fungus activity can enhance the purity of ores and if the metabolic wastes associated with the process are timely and well managed, it is possible degradation could be continuous in view of microbial exponential growth rate.

show abstract

Growth Profile and Hydrocarbonoclastic Potential of Microorganisms Isolated from Tarballs in the Bight of Bonny, Nigeria

Cited by 71 publications

References 14 publications

Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

Isolation and Identification of Plasmids of Bacteria from Petroleum Products Contaminated Soil

Phosphorus removal from Nigerias Agbaja iron ore by solubilization with Eurotium herbariorum

Contact Info

Product

Resources

About