Growth Promoting Activity of Annona muricata L. Leaf Extracts on Lactobacillus casei

Abstract

Soursop leaves are a source of phytochemical compounds, such as phenolic acids, flavonoids, hydrolyzable tannins, and acetogenins. These compounds can have several types of biological activities. Lactic acid bacteria can uptake phenolic compounds present in plants or fruits. The aim of the present work was to investigate the in vitro effect of hexane, acetone, methanolic, and aqueous extracts of soursop leaves (Annona muricata L.) on the growth, motility, and biofilm formation of Lactobacillus casei, and to determine compounds related to growth. The minimum concentration promoting growth, motility (swimming, swarming, and twitching), and biofilm-forming capacity (crystal violet) were evaluated. The results showed the growth-promoting capacity of acetone and aqueous extracts at low doses 25–50 mg/L, and an inhibition in the four extracts at higher doses of 100 mg/L. The L. casei growth is related to ellagic acid, quercetin rhamnoside, kaempferol dihexoside, quercetin hexoside, secoisolariciresinol, and kaempferol hexoside-rhamnoside. Hexane extract increased the three types of motility, while aqueous maintained swimming and twitching motility similar to control. The four extracts inhibited the biofilm formation capacity.

SOURSOP (Annona muricata) LEAF MEAL FED TO Clarias gariepinus PARENT INFLUENCED THE GROWTH AND EARLY DEVELOPMENT OF THE F1 GONADS

“…Annona muricata is known for its nutritional richness, containing vitamins, minerals, and bioactive compounds. It's conceivable that the nutritional profile of the fruit could indirectly influence the overall health and development of fish, including gonads (Meza-Gutiérrez et al, 2022). The bioactive compounds present in Annona muricata contain acetogenins, which have been studied for their potential health benefits (Mutakin et al, 2022).…”

Nutritional and Therapeutic Potential of Soursop

“…Excessive levels of free radicals induced oxidative stress, that could cause tissue, DNA, protein, and lipid damage, necessitating the consumption of dietary antioxidant substances that aid the body in neutralizing these free radicals [46]. Flavonoids and tannins are phenolic chemicals, their availability in soursop extracts may be vital for the reported free radical scavenging actions [47]. e leaves of soursop contain therapeutic active substances (e.g., alkaloids, coumarins, tannins, flavonoids, carbohydrates, phenols, terpenoids, and saponins that have antioxidant activity [32].…”