Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar

Kok, Andrew De-Xian; Abdullah, Wan Muhamad Asrul Nizam Wan; Tan, Ngai-Paing; Abdullah, Janna Ong; Sekeli, Rogayah; Wee, Chien-Yeong; Lai, Kok-Song

doi:10.1007/s13205-020-2118-5

Cited by 9 publications

(11 citation statements)

References 41 publications

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“…An earlier study on PF-68 in callus proliferation revealed that optimum concentration of PF-68 (0.04%) significantly enhanced callus proliferation of MR 219 (Kok et al, 2020 ). Meanwhile, high concentration of PF-68 (0.10%) was demonstrated to induce stress response (Kok et al, 2020 ). In this study, plant hormones were removed from the callus proliferation medium in order to investigate the mechanism governing the growth-promoting response of PF-68 as a plant additive without the interference of plant hormones.…”

Section: Resultsmentioning

confidence: 96%

Section: Growth-promoting Response Of Pf-68 Without the Presence Of Plant Hormonementioning

confidence: 97%

“…An earlier study made by Kok et al (2020) on PF-68 application has shown successful enhancement of a callus proliferation rate and a number of callus with root-like structure of MR 219 indica rice cultivar. Unfortunately, the underlying growth promoting mechanisms of PF-68 remains largely unknown.…”

Section: Introductionmentioning

confidence: 94%

“…Besides, the application of PF-68 was reported to enhance shoot regeneration in Citrus sinensis (Curtis and Mirkov, 2012 ), Pyrus communis (Dashti et al, 2012 ), Ricinus communis (Kulathuran and Narayanasamy, 2015 ), and Abelmoschus esculentus (Irshad et al, 2018 ). Notably, PF-68 was demonstrated to improve the growth of roots, callus, and protoplast of Solanum dulcamara (Kumar et al, 1992 ), shoot regeneration of recalcitrant Brassica napus embryos (Barbulescu et al, 2011 ), and callus proliferation of recalcitrant indica rice (Kok et al, 2020 ). This implies that PF-68 could be a good candidate for plant cell growth and regeneration improvement of the recalcitrant cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Pluronic F-68 Improves Callus Proliferation of Recalcitrant Rice Cultivar via Enhanced Carbon and Nitrogen Metabolism and Nutrients Uptake

et al. 2021

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Pluronic F-68 (PF-68) is a non-ionic surfactant used in plant tissue culture as a growth additive. Despite its usage as a plant growth enhancer, the mechanism underlying the growth-promoting effects of PF-68 remains largely unknown. Hence, this study was undertaken to elucidate the growth-promoting mechanism of PF-68 using recalcitrant MR 219 callus as a model. Supplementation of 0.04% PF-68 (optimum concentration) was shown to enhance callus proliferation. The treated callus recorded enhanced sugar content, protein content, and glutamate synthase activity as exemplified in the comparative proteome analysis, showing protein abundance involved in carbohydrate metabolism (alpha amylase), protein biosynthesis (ribosomal proteins), and nitrogen metabolism (glutamate synthase), which are crucial to plant growth and development. Moreover, an increase in nutrients uptake was also noted with potassium topping the list, suggesting a vital role of K in governing plant growth. In contrast, 0.10% PF-68 (high concentration) induced stress response in the callus, revealing an increment in phenylalanine ammonia lyase activity, malondialdehyde content, and peroxidase activity, which were consistent with high abundance of phenylalanine ammonia lyase, peroxidase, and peroxiredoxin proteins detected and concomitant with a reduced level of esterase activity. The data highlighted that incorporation of PF-68 at optimum concentration improved callus proliferation of recalcitrant MR 219 through enhanced carbohydrate metabolism, nitrogen metabolism, and nutrient uptake. However, growth-promoting effects of PF-68 are concentration dependent.

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Section: Resultsmentioning

confidence: 96%

Section: Growth-promoting Response Of Pf-68 Without the Presence Of Plant Hormonementioning

confidence: 97%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Pluronic F-68 Improves Callus Proliferation of Recalcitrant Rice Cultivar via Enhanced Carbon and Nitrogen Metabolism and Nutrients Uptake

et al. 2021

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“…The PAL is one of the rate-limiting enzymes, known to play a key role in secondary metabolite biosynthesis pathway. Secondary metabolites biosynthesis involves production of phenolic and avonoid compounds which plays an important role in regulation of cellular homeostasis in plants in response towards stress 33,34 .…”

Section: Discussionmentioning

confidence: 99%

Sodium Lignosulfonate Improves Shoot Growth of Oryza Sativa via Enhancement of Photosynthetic Activity and Reduction of Stress Response

Kok

Tan

Abdullah

et al. 2020

Preprint

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Lignosulfonate (LS) is a by-product obtained during sulfite pulping process and is commonly used as a growth enhancer in plant growth. However, the underlying growth promoting mechanism of LS on shoot growth remains largely unknown. Hence, this study was undertaken to determine the potential application of eco-friendly LS chelated ion complex (NaLS and CaLS) to enhance recalcitrant indica rice MR219 shoot growth and to elucidate its underlying growth promoting mechanisms. The NaLS was shown to be a better shoot growth enhancer as compared to CaLS, with optimum concentration of 300 mg/L. Subsequent comparative proteomic analysis revealed an increase of photosynthesis-related proteins and stress regulator proteins abundance in NaLS-treated rice as compared to MSO (control). Consistently, biochemical analyses showed a significant increase of rubisco activity, total chlorophyll, total sugar and total protein contents in NaLS-treated rice, implying NaLS role in empowering photosynthesis activities that led to plant growth enhancement. In addition, low level of peroxidase activity, malondialdehyde content and phenylalanine ammonia lyase activity were also observed in NaLS-treated rice. These results suggest that NaLS plays a role in modulating cellular homeostasis to provide a conducive cellular environment for plant growth. Taken together, NaLS improved shoot growth of recalcitrant MR219 rice by upregulation of photosynthetic activities and reduction of cellular stress leading to better plant growth.

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Calcium lignosulfonate modulates physiological and biochemical responses to enhance shoot multiplication in Vanilla planifolia Andrews

Thye

Abdullah

Ong-Abdullah

et al. 2023

Physiol Mol Biol Plants

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Growth promoting effects of Pluronic F-68 on callus proliferation of recalcitrant rice cultivar

Cited by 9 publications

References 41 publications

Pluronic F-68 Improves Callus Proliferation of Recalcitrant Rice Cultivar via Enhanced Carbon and Nitrogen Metabolism and Nutrients Uptake

Pluronic F-68 Improves Callus Proliferation of Recalcitrant Rice Cultivar via Enhanced Carbon and Nitrogen Metabolism and Nutrients Uptake

Sodium Lignosulfonate Improves Shoot Growth of Oryza Sativa via Enhancement of Photosynthetic Activity and Reduction of Stress Response

Calcium lignosulfonate modulates physiological and biochemical responses to enhance shoot multiplication in Vanilla planifolia Andrews

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