Growth Promoting Potential and Colonization Ability of Probiotics (Bacillus coagulans and Bacillus subtilis) on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

Abstract

The probiotic effects of Bacillus coagulans and Bacillus subtilis were studied on survival, growth, concentrations of basic biochemical constituents, activities of digestive enzymes, and their colony establishments in the gut of Macrobrachium rosenbergii post-larvae (PL). Eleven groups of PL (2.03±0.05 in length and 0.18±0.01g in weight), each consists of 35 individuals maintained in 25 L of ground water and fed ad libitum with fi ve serially diluted concentrations, 10 , 10 -7 and 10 -9 of B. coagulans, and B. subtilis incorporated diets containing 40% protein, for 45 days. Diet without incorporation of any of these probiotics was served as control. These probiotics were found to be alive in the respective feed even on day-15 after their formulations. Signifi cant improvement in survival, nutritional indices (weight gain, specifi c growth rate, food conversion ratio and protein effi ciency ratio), contents of basic biochemical constituents (total protein, amino acid, carbohydrate and lipid) and activities of digestive enzymes (protease, amylase and lipase) were observed (P<0.05), particularly in 10 -7 concentration of B. coagulans, and B. subtilis incorporated diets fed PL when compared with control. The biochemical confi rmation tests revealed that presence of Escherichia coli, Acetonobacter sp., Salmonella sp., and Pseudomonas sp., in the gut of control PL. In the gut of PL fed with B. coagulans incorporated diet, Acetonobacter sp., Salmonella sp., and Pseudomonas sp., were found to be competitively excluded, whereas, in the gut of PL fed with B. subtilis incorporated diet, Acetonobacter sp., and Salmonella sp., only were found to be excluded competitively. Actually, colonies of Bacillus sp., and Lactobacillus sp., were found to be establishment in the gut of PL fed with B. coagulans, and B. subtilis incorporated diets. Overall, these probiotics incorporated diets produced better growth and survival due to better FCR and activities of digestive enzymes, which in turn led to better nutritional profi le. Therefore they are recommended as feed additives for sustainable culture of M. rosenbergii.

WITHDRAWN: Effects of dietary Bacillus pumilus on the growth performance, intestinal health, lipid metabolism, and mTOR signaling pathway of juvenile Coho salmon (Oncorhynchus kisutch)

“…Studies have revealed that most of the proteins encoded by the B. velezensis genome are involved in carbohydrate and amino acid transport and metabolism [33], thereby decomposing macromolecular nutrients into small molecular polypeptides, amino acids, and fatty acids, among others, which are conducive to absorption, and improving the utilization rate of nutrients in sh feed. Additionally, probiotics can enhance growth performance by producing various digestive enzymes [34]. The B. pumilus strain used in this study was preliminarily selected for its high production of protease, which can effectively decompose and utilize nutritional proteins in animal and plant protein sources.…”

Growth and survival promotion of a probiotic bacterium Enterococcus durans enriched Artemia nauplii on the prawn Macrobrachium rosenbergii

“…Similar elevations in activities of protease, amylase and lipase have been reported by Jain et al, [39,40] in M. rosenbergii PL fed with E. gallinarum and E. hirae enriched Artemia nauplii. Elevated activities of protease, amylase and lipase have also been recorded in M. rosenbergii PL fed with LactoBasil ® plus, ViBact*, L. sporogenes, Bacillus subtilis, S. cerevisiae, Lactobacillus brevis, Lactobacillus fermentum, B. coagulans and B. licheniformis supplemented feeds [21,22,[24][25][26][27][28][29][30]. It has been reported that a significant increase in activities of digestive enzymes in Penaeus vannamei larvae when using B. coagulans and B. subtilis in its feeding regime [62][63][64].…”

“…They have competitively exclude pathogenic bacteria by production of inhibitory compounds in an ecofriendly manner, increase immune response, stress resistance, disease tolerance, maintain water quality, encourage reproduction and offer better growth and survival [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Probiotics increase digestibility of nutrients and feed efficiency by producing some exogenous enzymes, which increases activities of endogenous enzymes (amylase, protease and lipase) to hydrolyze nutrients, and effectively improve growth, survival and nutritional quality of the host [21][22][23][24][25][26][27][28][29][30].…”

Effect of Beneficial Colonization of Bacillus coagulans NRS 609 on Growth Performance, Intestinal Health, Antioxidant Capacity, and Immune Response of Common Carp (Cyprinus carpio L.)

“…In aquaculture, Bacillus coagulans, a spore-forming gram-positive bacterium, has frequently been applied as a feed additive due to its combination of lactic acid bacteria and Bacillus properties [19,20]. Moreover, B. coagulans is known to endure high temperatures, acidity, and bile salts and inhibit enteropathogens [21][22][23][24].…”