Growth promotion and biological control of root‐knot nematodes in micropropagated banana during the nursery stage by treatment with specific bacterial strains

Abstract

The effect of strains EPS291 and EPS817 of Pseudomonas fluorescens to improve plant growth and nutrition as well as to reduce root‐knot nematode in roots was investigated in micropropagated banana. Plantlets, cultured under commercial nursery conditions, were treated twice with bacteria after transplanting from in vitro conditions. Bacterial application was performed by drenching bacterial suspension containing approximately 108 CFU (colony‐forming units) g−1 of substrate. Both strains significantly increased aerial fresh weight, plant length and leaf area, while the effect on other growth parameters depended on the strain. N content was also significantly increased after both bacteria treatments. A second experiment was performed to determine the potential biocontrol effect against the root‐knot nematode Meloidogyne javanica. Following the same experimental conditions, Meloidogyne inoculum containing 10 500 nematodes was applied 15 days after the second bacteria treatment. At the end of the experiment, 135 days later, nematode reproduction in roots of bacterised bananas was significantly reduced. In both experiments, the inoculated bacteria colonised the root system and population levels in root, 5.5 months after transplanting were 6.8 × 103 to 1.6 × 104 CFU g−1 fresh weight of root. The potential use of this technology to improve hardening stages in banana plant production in commercial nurseries is discussed.

Anatomy and morphology of photinia (Photinia × fraseri Dress) in vitro plants inoculated with rhizobacteria

“…These results are similar to those found in PGPR inoculation of potato, tomato, grapevine, banana and some Prunus in vitro cultures (Frommel et al 1991;Ait-Barka et al 2002;Nowak and Shulaev 2003;Rodríguez-Romero et al 2008;Russo et al 2008).…”

“…Biotechnological techniques, such as the application of plant growth-promoting rhizobacteria (PGPR), have been effective in reducing the biochemical, anatomical and morphological modification of in vitro propagules in diverse species (Frommel et al 1991;Ait-Barka et al 2002;Nowak and Shulaev 2003;Larraburu et al 2007;Rodríguez-Romero et al 2008;Russo et al 2008). PGPR are a group of rhizosphere-colonizing bacteria that induce changes in inoculated plants, which can improve plant growth.…”

Shoot the Message, Not the Messenger—Combating Pathogenic Virulence in Plants by Inhibiting Quorum Sensing Mediated Signaling Molecules

“…It enhances induced biotic and abiotic stress resistance (Badosa and Montesinos, 2008; Lugtenberg and Kamilova, 2009; Senthilkumar et al, 2011). Endophytes improve plants' health mainly through siderophore production, thereby enabling biological nitrogen fixation (Ngamau et al, 2012), phosphate solubilization (Andrade et al, 2014), and effective transport of iron (Fe) and zinc (Zn) from the rhizosphere region by ZIP transporters (Krithika and Balachandar, 2016).…”

Aislamiento y caracterización de bacterias de la rizosfera de banano (Musa sp.) en República Dominicana y selección de cepas para el desarrollo de biofertilizantes = Isolation and characterization of banana (Musa sp.) rizospheric bacteria in Dominican Republic and selection of strains for the development of biofertilizers

“…Existe poca bibliografía científica sobre el uso de PGPR para la producción de plantas de banano para el trasplante. Sin embargo Rodríguez-Romero et al (2008) realizaron estudios sobre plantas de banano inoculadas con dos cepas de Pseudomonas fluorescens obteniendo que las mismas inducían un aumento en el peso fresco aéreo, en la longitud del tallo y el área de la hoja de la planta, así como un aumento del contenido de nitrógeno. Además la inoculación de estas cepas como biocontroladoras redujeron la incidencia de nematodo Meloidogyne javanica.…”

“…Para seleccionar las cepas a incluir en el ensayo, el criterio primario fue también la producción de sideróforos, ya que según García-Gutiérrez et al 2012 En el ensayo de cámara los resultados obtenidos con las bacterias del género Bacillus, concretamente del grupo RFLP XXVI y también con un Rhizobium massiliae (grupo RFLP XXXIX) superaron claramente a los obtenidos con Pseudomonas. Otros autores que han trabajado con RSI en banano, aunque no contra sigatoka (Rodríguez-Romero et al 2008;Harish et al, 2008Harish et al, , 2009Fishal et al, 2010)…”