Growth promotion effect of red ginseng dietary fiber to probiotics and transcriptome analysis of Lactiplantibacillus plantarum

Yu, Hye-Young; Rhim, Dong-Bin; Kim, Sang-Kyu; Ban, O‐Hyun; Oh, Sangki; Seo, Jiho; Hong, Soon-Ki

doi:10.1016/j.jgr.2022.09.003

Cited by 8 publications

(6 citation statements)

References 38 publications

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“…However, most studies of red ginseng components have focused on the effects of ginseng extract. In contrast, the effects of dietary fibers derived from red ginseng have not been explored fully ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Red Ginseng Dietary Fiber Shows Prebiotic Potential by Modulating Gut Microbiota in Dogs

Song,

Lee,

et al. 2023

Microbiol Spectr

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Section: Introductionmentioning

confidence: 99%

Red Ginseng Dietary Fiber Shows Prebiotic Potential by Modulating Gut Microbiota in Dogs

Song,

Lee,

et al. 2023

Microbiol Spectr

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“…The by-products of the processing red ginseng still contain several types of bioactive components, such as acidic polysaccharides and dietary fiber, as well as the remaining ginsenosides ( Park and Kim, 2006 ). RGDF is a byproduct composed of approximately 31% dietary fiber (314.3 mg/g) and 0.66% ginsenoside (6.63 mg/g of total ginsenosides) ( Yu et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…3-Phosphoglyceric acid is one derivative that is especially important for serine and cysteine biosynthesis. A recent study demonstrated that L. plantarum supplemented with 2% RGDF upregulates the expression of genes involved in serine (sdhA, sdhB, and sdaC) and cysteine metabolism (cysE) ( Yu et al, 2022 ). Although further verification of the changes in specific metabolic and physiologic mechanisms is required, our results support the view that RGDF supplementation alters cellular and metabolic processes.…”

Section: Discussionmentioning

confidence: 99%

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Red ginseng dietary fiber promotes probiotic properties of Lactiplantibacillus plantarum and alters bacterial metabolism

et al. 2023

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Korean red ginseng has been widely used as an herbal medicine. Red ginseng dietary fiber (RGDF) is a residue of the processed ginseng product but still contains bioactive constituents that can be applied as prebiotics. In this study, we evaluated changes on fermentation profiles and probiotic properties of strains that belong to family Lactobacillaceae with RGDF supplementation. Metabolomic analyses were performed to understand specific mechanisms on the metabolic alteration by RGDF and to discover novel bioactive compounds secreted by the RGDF-supplemented probiotic strain. RGDF supplementation promoted short-chain fatty acid (SCFA) production, carbon source utilization, and gut epithelial adhesion of Lactiplantibacillus plantarum and inhibited attachment of enteropathogens. Intracellular and extracellular metabolome analyses revealed that RGDF induced metabolic alteration, especially associated with central carbon metabolism, and produced RGDF-specific metabolites secreted by L. plantarum, respectively. Specifically, L. plantarum showed decreases in intracellular metabolites of oleic acid, nicotinic acid, uracil, and glyceric acid, while extracellular secretion of several metabolites including oleic acid, 2-hydroxybutanoic acid, hexanol, and butyl acetate increased. RGDF supplementation had distinct effects on L. plantarum metabolism compared with fructooligosaccharide supplementation. These findings present potential applications of RGDF as prebiotics and bioactive compounds produced by RGDF-supplemented L. plantarum as novel postbiotic metabolites for human disease prevention and treatment.

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“…In our present study, we observed that all of the bacteria in the CS group exhibited higher proliferation rates and increased adhesion capacity compared to the UCS controls in each intestinal epithelia model system, following a 24 h incubation. Of note, when L. plantarum IDCC3501 was cultured with RGDF, the component was found to promote the growth of IDCC3501 by acting as a proliferation enhancer [ 29 ]. Moreover, arginine facilitates the proliferation of Streptococcus thermophilus T1C2 by protecting the strain against intracellular acid stress [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Multilayer Coating with Red Ginseng Dietary Fiber Improves Intestinal Adhesion and Proliferation of Probiotics in Human Intestinal Epithelial Models

Son,

Kwon,

Son

et al. 2023

J. Microbiol. Biotechnol.

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To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains ( Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng , using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.

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Growth promotion effect of red ginseng dietary fiber to probiotics and transcriptome analysis of Lactiplantibacillus plantarum

Cited by 8 publications

References 38 publications

Red Ginseng Dietary Fiber Shows Prebiotic Potential by Modulating Gut Microbiota in Dogs

Red Ginseng Dietary Fiber Shows Prebiotic Potential by Modulating Gut Microbiota in Dogs

Red ginseng dietary fiber promotes probiotic properties of Lactiplantibacillus plantarum and alters bacterial metabolism

Multilayer Coating with Red Ginseng Dietary Fiber Improves Intestinal Adhesion and Proliferation of Probiotics in Human Intestinal Epithelial Models

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