Growth promotion of Chlorella vulgaris by modification of nitrogen source composition with symbiotic bacteria, Microbacterium sp. HJ1

Kim, Hyun‐Joong; Choi, Yong‐Keun; Jeon, Hyeon Jin; Bhatia, Shashi Kant; Kim, Yong Hyun; Kim, Yun‐Gon; Choi, Kwon Young; Kim, Hyung Joo; Lee, Sang Hyun; Lee, Yoo Kyung; Yang, Yung‐Hun

doi:10.1016/j.biombioe.2015.01.012

Cited by 29 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, it has been suggested that ammonium may have a different effect than nitrate, because nitrate use requires an energetically costly reduction [36,37], potentially lowering the amount of photosynthates transferred by symbionts to the host [38]. Altogether, these results suggest that there is a need to better understand the physiological processes and nutrient fluxes within the coral-dinoflagellate association, which will shed light on the functioning of other nutrient-sharing symbioses also affected by nutrient enrichments, such as the plantmychorrizae, or chlorella-mycobacterium associations [39,40]. Therefore, the first aim of this study was to test the effect of nitrogen enrichment, either supplied in the form of ammonium or nitrate, on the acquisition of carbon and allocation of photosynthates in the tropical coral Stylophora pistillata in symbiosis with Symbiodinium clade A1.…”

Section: Introductionmentioning

confidence: 96%

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH ₄ ⁺ and NO ₃ ⁻ supply

et al. 2015

View full text Add to dashboard Cite

Anthropogenic nutrient enrichment affects the biogeochemical cycles and nutrient stoichiometry of coastal ecosystems and is often associated with coral reef decline. However, the mechanisms by which dissolved inorganic nutrients, and especially nitrogen forms (ammonium versus nitrate) can disturb the association between corals and their symbiotic algae are subject to controversial debate. Here, we investigated the coral response to varying N : P ratios, with nitrate or ammonium as a nitrogen source. We showed significant differences in the carbon acquisition by the symbionts and its allocation within the symbiosis according to nutrient abundance, type and stoichiometry. In particular, under low phosphate concentration (0.05 mM), a 3 mM nitrate enrichment induced a significant decrease in carbon fixation rate and low values of carbon translocation, compared with control conditions (N : P ¼ 0.5 : 0.05), while these processes were significantly enhanced when nitrate was replaced by ammonium. A combined enrichment in ammonium and phosphorus (N : P ¼ 3 : 1) induced a shift in nutrient allocation to the symbionts, at the detriment of the host. Altogether, these results shed light into the effect of nutrient enrichment on reef corals. More broadly, they improve our understanding of the consequences of nutrient loading on reef ecosystems, which is urgently required to refine risk management strategies.

show abstract

Section: Introductionmentioning

confidence: 96%

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH ₄ ⁺ and NO ₃ ⁻ supply

et al. 2015

View full text Add to dashboard Cite

show abstract

“…1.19-fold increment in airlift photobioreactor (dry weight) AEX medium; in air bubble photobioreactor [ 61 ] Botryococcus braunii and Candidatus Phycosocius bacilliformis 1.8-fold in the biomass and 1.5-fold improvement in total hydrocarbon yield Vitamin containing modified media of TSP [ 62 ] Microbacterium sp. and Chlorella vulgaris 1.66-fold increment in biomass (cell dry weight) Modified MBG medium [ 63 ] Acidovorax facilis and Scenedesmus obliquus 1.24-fold in biomass productivity and 1.29-fold in lipid productivity BG 11 medium [ 64 ] Chlorella and Azospirillum brasilense Increased the cell size up to 1.62-fold in Chlorella vulgaris , and total lipid yield increased up to 1.5–3.8 times in varying Chlorella spp. Sterile mineral medium (C30) and co-cultivated in the alginate beads [ 65 ] Chlorella and Azospirillum brasilense 2.1-fold in fresh weight and 24-fold increase in the dry weight of alginate beads containing the per gram of microbial biomass Residual water medium (synthetic wastewater medium) and co-cultivated in alginate beads [ 66 ] Stappia sp., Pelagibaca bermudensis and Tetraselmis striata 1.5–2-fold improvement in biomass productivity O3 Media with higher T. striata concentration as inoculation [ 12 ] P. bermudensis and T. striata 1.2–3-fold improvement in biomass productivity in varying stressors; 1.5-fold improvement in lipid productivity O3 media with varying physicochemical conditions This study …”

Section: Resultsmentioning

confidence: 99%

Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity

Patidar

Kim

et al. 2018

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundAmelioration of biofuel feedstock of microalgae using sustainable means through synthetic ecology is a promising strategy. The co-cultivation model (Tetraselmis striata and Pelagibaca bermudensis) was evaluated for the robust biofuel production under varying stressors as well as with the selected two-stage cultivation modes. In addition, the role of metabolic exudates including the quorum-sensing precursors was assessed.ResultsThe co-cultivation model innovated in this study supported the biomass production of T. striata in a saline/marine medium at a broad range of pH, salinity, and temperature/light conditions, as well as nutrient limitation with a growth promotion of 1.2–3.6-fold. Hence, this developed model could contribute to abiotic stress mitigation of T. striata. The quorum-sensing precursor dynamics of the growth promoting bacteria P. bermudensis exhibited unique pattern under varying stressors as revealed through targeted metabolomics (using liquid chromatography–mass spectrometry, LC–MS). P. bermudensis and its metabolic exudates mutually promoted the growth of T. striata, which elevated the lipid productivity. Interestingly, hydroxy alkyl quinolones independently showed growth inhibition of T. striata on elevated concentration. Among two-stage cultivation modes (low pH, elevated salinity, and nitrate limitation), specifically, nitrate limitation induced a 1.5 times higher lipid content (30–31%) than control in both axenic and co-cultivated conditions.ConclusionPelagibaca bermudensis is established as a potential growth promoting native phycospheric bacteria for robust biomass generation of T. striata in varying environment, and two-stage cultivation using nitrate limitation strategically maximized the biofuel precursors for both axenic and co-cultivation conditions (T and T-PB, respectively). Optimum metabolic exudate of P. bermudensis which act as a growth substrate to T. striata surpasses the antagonistic effect of excessive hydroxy alkyl quinolones [HHQ, 4-hydroxy-2-alkylquinolines and PQS (pseudomonas quorum signal), 2-heptyl-3-hydroxy-4(1H)-quinolone].Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1097-9) contains supplementary material, which is available to authorized users.

show abstract

“…HJ1, isolated from Chlorella vulgaris , shows 99% identity with a wastewater isolated bacterium - Microbacterium kitamiense . This bacterium has been proven to promote Chlorella growth in the modified livestock wastewater 29 . Interestingly, we assembled a complete genome of the associated bacterium Microbacterium chocolatum that also belongs to Microbacterium genus in Dachan.…”

Section: Discussionmentioning

confidence: 99%

Sequencing and comparative analysis of three Chlorella genomes provide insights into strain-specific adaptation to wastewater

Jiang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Microalgal Chlorella has been demonstrated to process wastewater efficiently from piggery industry, yet optimization through genetic engineering of such a bio-treatment is currently challenging, largely due to the limited data and knowledge in genomics. In this study, we first investigated the differential growth rates among three wastewater-processing Chlorella strains: Chlorella sorokiniana BD09, Chlorella sorokiniana BD08 and Chlorella sp . Dachan, and the previously published Chlorella sorokiniana UTEX 1602, showing us that BD09 maintains the best tolerance in synthetic wastewater. We then performed genome sequencing and analysis, resulting in a high-quality assembly for each genome with scaffold N50 > 2 Mb and genomic completeness ≥91%, as well as genome annotation with 9,668, 10,240, 9,821 high-confidence gene models predicted for BD09, BD08, and Dachan, respectively. Comparative genomics study unravels that metabolic pathways, which are involved in nitrogen and phosphorus assimilation, were enriched in the faster-growing strains. We found that gene structural variation and genomic rearrangement might contribute to differential capabilities in wastewater tolerance among the strains, as indicated by gene copy number variation, domain reshuffling of orthologs involved, as well as a ~1 Mb-length chromosomal inversion we observed in BD08 and Dachan. In addition, we speculated that an associated bacterium, Microbacterium chocolatum , which was identified within Dachan, play a possible role in synergizing nutrient removal. Our three newly sequenced Chlorella genomes provide a fundamental foundation to understand the molecular basis of abiotic stress tolerance in wastewater treatment, which is essential for future genetic engineering and strain improvement.

show abstract

Growth promotion of Chlorella vulgaris by modification of nitrogen source composition with symbiotic bacteria, Microbacterium sp. HJ1

Cited by 29 publications

References 19 publications

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH ₄ ⁺ and NO ₃ ⁻ supply

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH ₄ ⁺ and NO ₃ ⁻ supply

Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity

Sequencing and comparative analysis of three Chlorella genomes provide insights into strain-specific adaptation to wastewater

Contact Info

Product

Resources

About

Growth promotion of Chlorella vulgaris by modification of nitrogen source composition with symbiotic bacteria, Microbacterium sp. HJ1

Cited by 29 publications

References 19 publications

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH 4 + and NO 3 − supply

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH 4 + and NO 3 − supply

Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity

Sequencing and comparative analysis of three Chlorella genomes provide insights into strain-specific adaptation to wastewater

Contact Info

Product

Resources

About

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH ₄ ⁺ and NO ₃ ⁻ supply

New insights into carbon acquisition and exchanges within the coral–dinoflagellate symbiosis under NH ₄ ⁺ and NO ₃ ⁻ supply