Growth promotion of Bifidobacterium and Lactobacillus species by proteinaceous hydrolysates derived from poultry processing leftovers

Abstract

Summary Bacterial growth media represent a high cost in industrial applications, and for this reason, it is economically important to find less expensive supplements to replace the traditional ones. In the present work, peptide hydrolysates obtained from poultry meat and bone residues (functional animal protein [FAP]) and from feathers (functional feather protein [FFP]) were studied to determine their ability for the production of microbial biomass with improved viability. The results obtained were compared with those obtained with other supplement nutritive compounds used in fermentation growth media. The molecular composition of the hydrolysates in terms of total and soluble nitrogen, molecular weight distribution, total and free amino acids, was determined. The growth and cellular state of Bifidobacterium and Lactobacillus strains were studied by turbidimetric measurements and direct count by fluorescence microscopy. Overall, this study suggested that by‐products from poultry industry provide a good alternative to substitute expensive supplements for growth of Lactobacillus and Bifidobacterium with a high level of viability.

Replacement of mechanically deboned chicken meat with its protein hydrolysate in mortadella-type sausages

“…Furthermore, the mortadella-type sausages containing MDCM protein hydrolysates also had higher (P < 0.05) LAB counts on days 15 and 45 of storage when compared to those of the CT. Lazzi et al (2011) showed that the addition of protein hydrolysate obtained from the poultry industry promoted the growth of a wide range of Bifidobacterium species of human origin. Recently, Lazzi et al (2013) also reported that the growth of Lactobacillus species could also be improved by the supplementation with proteinaceous hydrolysates derived from poultry processing leftovers. These studies showed that protein hydrolysates from poultry meat are a promising functional food, and this is probably one of the reasons why the treatments with MDCM protein hydrolysate addition showed higher LAB counts when compared to those of the control treatment.…”

Bioavailability and prebiotic potential of Carapax Trionycis, a waste from soft‐shelled turtle processing

“…Soluble protein fractions in Carapax Trionycis could also contribute to its prebiotic potential. Some studies have reported that lactobacillus and bifidobacteria growth was stimulated by bone and meat residues due to their high peptides and free amino acids . In fact, lactobacilli is typical multiple amino acids auxotrophs bacterium, and its growth is closely related to the amount and quality of amino acids in the culture medium .…”

“…Calcium in animal bones mainly exists in the forms of hydroxyapatite crystals and amorphous calcium hydrogen phosphate wrapped by collagen fibers, but dense tissues cannot be absorbed easily by the body . Much effort has been made to modify bone, including high pressure, enzymolysis, and acid hydrolysis methods . However, high‐pressure cooking in the presence of water can lead to the loss of a large amount of nutrients .…”

“…Lazzi et al . reported that the enzymatic hydrolysate of poultry bone and meat shavings significantly stimulated the proliferation of bifidobacteria and lactobacillus . Tang et al .…”

“…[14][15][16] Much effort has been made to modify bone, including high pressure, enzymolysis, and acid hydrolysis methods. [17][18][19] However, high-pressure cooking in the presence of water can lead to the loss of a large amount of nutrients. 17 Enzyme-hydrolyzed methnology easily produces a bad flavor in the resulting bone products.…”