Growth promotory potential of the cold adapted diazotroph Pseudomonas migulae S10724 against native green gram (Vigna radiata (L.) Wilczek)

Abstract

It is being confirmed previously the atmospheric nitrogen fixing ability of the cold adapted Pseudomonas migulae S10724 strain at the fluctuating temperatures. Therefore, net house bioinoculation experiment was performed to determine the effectiveness of inoculation of strain S10724 on the growth enhancement of native green gram (Vigna radiata L. Wilczek). The strain significantly (p < 0.05) stimulated the growth of roots (45.3 %) and shoots (45.6 %) of green gram plants. Furthermore, other growth related parameters viz. fresh and dry weight was also found to be increased significantly. Treated plants typically showed more obvious modifications in their biochemical status also. The total chlorophyll and nitrate reductase activity was increased in S10724 inoculated plant as compared to the control one. Moreover, in vitro seed germination assay revealed that the germination was increased in S10724 strain treated seeds by 22 % at 25 °C while 25 % at 12 °C unlikely to respective controls. The results suggest that P.migulae S10724 strain is a potential plant growth promoting bacterium for legume under fluctuating temperature ranges and therefore, could be used effectively as a low cost bioinoculant in Himalayan agricultural belt successfully.

Comparative plant growth promoting potential of psychrotolerant diazotrophs, Pseudomonas sp. JJS2 and Enterobacter sp. AAB8 against native Cajanus cajan (L.) and Eleusine coracana (L.)

“…Katiyar and Goel (2003) assessed the inoculation effect of phosphate-solubilizing cold-tolerant mutant of P. fluorescens on mungbean in sterilized and unsterilized soil and observed that inoculated plants resulted in better plant growth in both soils. Moreover, it is also reported that inoculation of the diazotrophic bacterial strains significantly increased the biomass of plants and also had a significant impact on the total N in plant tissues as compared to uninoculated control (Suyal et al, 2014a, Yim et al, 2009. These reports further corroborates with our findings with respect to efficacy of a native diazotrophic strain in improving crop growth parameters and hence increasing crop yield.…”

“…Significance of rhizosphere microbiome under several biotic and abiotic stresses has been reviewed by Mendes et al (2013). Previously presence of psychrotolerant diazotrophs of Western Indian Himalayas was reported by Suyal et al (2014a). We, therefore, conducted the present study which focuses on the isolation, identification and application of native psychrotolerant diazotrophic strains under natural fluctuating temperatures which could be explored against the individual or mixed cropping of native E. coracana and C. cajan in upper Himalayan agricultural lands.…”

Bacterial diversity and community structure of Western Indian Himalayan red kidney bean (Phaseolus vulgaris) rhizosphere as revealed by 16S rRNA gene sequences

“…Recently, Sanchez et al (2014) characterized twenty different plant growth promoting bacteria from the rhizosphere of Phaseolus vulgaris. Previous studies confirmed that Himalayan soils have bacterial community with a tremendous potential of biodegradation (Soni et al 2008) and plant growth promotion (Kumar et al 2014;Suyal et al 2014a). Previous studies confirmed that Himalayan soils have bacterial community with a tremendous potential of biodegradation (Soni et al 2008) and plant growth promotion (Kumar et al 2014;Suyal et al 2014a).…”

Plant Beneficial Endophytic Bacteria from the Ethnomedicinal Mussaenda roxburghii (Akshap) of Eastern Himalayan Province, India

“…JJS2 of Western Indian Himalayas showed good growth on N2-free media at room temperature; their nif H fragment amplification confirms their diazotrophic nature [27]. An inoculation of the diazotrophs significantly improves the crop productivity and biomass in contrast to uninoculated control crop [28].…”