Growth Rate and Biofilm Formation Ability of Clinical and Laboratory-Evolved Colistin-Resistant Strains of Acinetobacter baumannii

Farshadzadeh, Zahra; Taheri, Behrouz; Rahimi, Sara; Shoja, Saeed; Pourhajibagher, Maryam; Haghighi, Mehrdad; Bahador, Abbas

doi:10.3389/fmicb.2018.00153

Cited by 46 publications

(34 citation statements)

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“…The high emergence rates of multidrug resistance (MDR) and extensively drug resistance (XDR) among A. baumannii isolates have led them to be considered as one of the top seven pathogens posing threats to medical and health systems (1,2). In addition to antibiotic resistance, biofilm formation is another characteristic leading to the survival of A. baumannii in the presence of antibiotics and high stresses (1)(2)(3). It was revealed that the difference in biofilm formation capacity among international clonal (IC) types of A. baumannii could affect the spreading of this microorganism (4).…”

Section: Introductionmentioning

confidence: 99%

The Relationship Between Antibiotic Resistance Phenotypes and Biofilm Formation Capacity in Clinical Isolates of Acinetobacter baumannii

Rahimi

Farshadzadeh

Taheri

et al. 2018

Jundishapur J Microbiol

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Background: Understanding of the biological factors responsible for prevalence and persistence of Acinetobacter baumannii in hospital settings is critical to prevent and control the corresponding nosocomial infections. Objectives: This study was conducted to investigate whether the biofilm-forming ability is associated with the emergence of different antibiotic resistance phenotypes [multidrug resistance (MDR)/extensively drug resistance (XDR) and non-MDR] of A. baumannii. Methods: The capacities of biofilm formation in 80 clinical A. baumannii strains isolated from hospitalized burn patients in Bushehr, Iran, were assessed using the crystal violet staining. Results: The statistical analysis of the relationship between biofilm-forming ability and antibiotic resistance phenotypes among all clinical A. baumannii strains using one-way ANOVA test indicated that biofilm formation capacity of non-MDR A. baumannii isolates was significantly higher than that of MDR and XDR ones (P < 0.001), suggesting an inverse relationship between biofilm formation capacity and the acquisition of MDR/XDR phenotypes. Major international clonal types (ICI and ICII) also exhibited such a significant relationship (P < 0.0001). However, the investigation of A. baumannii IC variants showed no significant relationship between these phenotypes. Conclusions: Given that non-MDR A. baumannii isolates in major IC types were observed to form a strong biofilm compared to MDR/XDR ones, it seems that biofilm may play a key role in the persistence and survival of A. baumannii isolates with an inadequate level of antibiotic resistance. Furthermore, the results showed that the relationship between biofilm and antibiotic resistance phenotypes might be affected by the IC types (major IC types or IC variants).

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Section: Introductionmentioning

confidence: 99%

The Relationship Between Antibiotic Resistance Phenotypes and Biofilm Formation Capacity in Clinical Isolates of Acinetobacter baumannii

Rahimi

Farshadzadeh

Taheri

et al. 2018

Jundishapur J Microbiol

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“…Pilus biosynthesis is important during the initial biofilm formation period, and this process depends on the csuA/BABCDE system (Gaddy & Actis, 2009;Longo, Vuotto, & Donelli, 2014). Biofilm rates (Farshadzadeh et al, 2018). Zea et al (2017) reported that E. coli cells in space accounted for 37% of the volume of the control group.…”

Section: Hensley (2010) Reported Similar Results Showing That Clinicmentioning

confidence: 99%

“…Biofilm formation in A. baumannii requires expression of biofilm‐related genes, such as csuA/B/D . Additionally, changes in the biofilm formation ability are probably associated with changes in growth rates (Farshadzadeh et al, ). Zea et al () reported that E. coli cells in space accounted for 37% of the volume of the control group.…”

Section: Discussionmentioning

confidence: 99%

“…The mission will require astronauts to stay on the space station for at least 180 days. China has also launched Shenzhou I, Shenzhou III, andShenzhou IV spacecraft in 1999-2002, and for academic purposes, Streptomyces fradiae was harbored on these spacecraft (Fang, Zhao, & Gu, 2005). A few years later, 15 strains of microorganisms were harbored on the Shenzhou VIII spacecraft, and 2 years later, the Shenzhou X spacecraft, which carried nine different microorganisms, was launched.…”

Section: Sistance Of Staphylococcus Epidermidis Klebsiella Pneumoniamentioning

confidence: 99%

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Decreased biofilm formation ability of Acinetobacter baumannii after spaceflight on China's Shenzhou 11 spacecraft

Zhao

Zhang

et al. 2018

MicrobiologyOpen

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China has prepared for construction of a space station by the early 2020s. The mission will require astronauts to stay on the space station for at least 180 days. Microbes isolated from the International Space Station (ISS) have shown profound resistance to clinical antibiotics and environmental stresses. Previous studies have demonstrated that the space environment could affect microbial survival, growth, virulence, biofilms, metabolism, as well as their antibiotic‐resistant phenotypes. Furthermore, several studies have reported that astronauts experience a decline in their immunity during long‐duration spaceflights. Monitoring microbiomes in the ISS or the spacecraft will be beneficial for the prevention of infection among the astronauts during spaceflight. The development of a manned space program worldwide not only provides an opportunity to investigate the impact of this extreme environment on opportunistic pathogenic microbes, but also offers a unique platform to detect mutations in pathogenic bacteria. Various microorganisms have been carried on a spacecraft for academic purposes. Acinetobacter baumannii is a common multidrug‐resistant bacterium often prevalent in hospitals. Variations in the ability to cope with environmental hazards increase the chances of microbial survival. Our study aimed to compare phenotypic variations and analyze genomic and transcriptomic variations in A. baumannii among three different groups: SS1 (33 days on the Shenzhou 11 spacecraft), GS1 (ground control), and Aba (reference strain). Consequently, the biofilm formation ability of the SS1 strain decreased after 33 days of spaceflight. Furthermore, high‐throughput sequencing revealed that some differentially expressed genes were downregulated in the SS1 strain compared with those in the GS1 strain. In conclusion, this present study provides insights into the environmental adaptation of A. baumannii and might be useful for understanding changes in the opportunistic pathogenic microbes on our spacecraft and on China's future ISS.

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“…Biofilm-related outer membrane lipoprotein (pgaB) was found to be upregulated in colistin resistant strains (10). However, another study showed that mutations involved in colistin resistance phenotype downregulate the expression of biofilm-associated genes of colistin-resistant isolates (11).…”

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confidence: 99%

Biofilm Formation of Acinetobacter baumannii Under in vitro and in vivo Colistin Exposure

Özer¹,

Vatansever²,

Doğan³

et al. 2019

Infect Dis Clin Microbiol

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A cinetobacter baumannii is one of the most pathogenic nosocomial infection agents because of its extreme resistance to almost all known antibiotics and host immune responses (1). The emergence of colistin-resistance in A. baumannii has been reported throughout the world (2, 3). Biofilm formation ability enhances virulence of A. baumannii by increasing survival of cells in unfavourable environmental conditions, such as underexposure of disinfectants, antibiotics or attack of immune cells (4, 5). Antibiotic-resistant phenotypes This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License.

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Growth Rate and Biofilm Formation Ability of Clinical and Laboratory-Evolved Colistin-Resistant Strains of Acinetobacter baumannii

Cited by 46 publications

References 47 publications

The Relationship Between Antibiotic Resistance Phenotypes and Biofilm Formation Capacity in Clinical Isolates of Acinetobacter baumannii

The Relationship Between Antibiotic Resistance Phenotypes and Biofilm Formation Capacity in Clinical Isolates of Acinetobacter baumannii

Decreased biofilm formation ability of Acinetobacter baumannii after spaceflight on China's Shenzhou 11 spacecraft

Biofilm Formation of Acinetobacter baumannii Under in vitro and in vivo Colistin Exposure

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